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F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
The recent application of 19F NMR in the study of biomolecular structure and dynamics has made it a potentially attractive...
Using temperature coefficients to support resonance assignment of intrinsically disordered proteins
Using temperature coefficients to support resonance assignment of intrinsically disordered proteins
The resonance assignment of large intrinsically disordered proteins (IDPs) is difficult due to the low dispersion of chemi...
Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins
Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins
Inclusion of residual dipolar couplings (RDCs) during the early rounds of protein structure determination requires use of ...
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements ...
N-detected TROSY for H-N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality
N-detected TROSY for H-N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality
Intrinsically disordered proteins and protein regions are central to many biological processes but difficult to characteri...
Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
Exploring the biochemical landscape of bacterial medium with pyruvate as the exclusive carbon source for NMR studies
The use of Escherichia coli for recombinant protein production is a cornerstone in structural biology, particularly for nu...
Improvement in protein HSQC spectra from addition of betaine
Improvement in protein HSQC spectra from addition of betaine
Addition of glycine betaine up to 1 M gave rise to increased intensity for some weak signals in the HSQC spectra ...
Terminal spin labeling of xylotriose strongly affects interactions in the active site of xylanase BcX
Terminal spin labeling of xylotriose strongly affects interactions in the active site of xylanase BcX
Paramagnetic probes provide long-range distance information and report on minor conformations of biomacromolecules. Howeve...
A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles
A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles
The investigation of structural propensities of proteins is essential for understanding how they function at the molecular...
Quantifying protein-drug lifetimes in human cells by F NMR spectroscopy
Quantifying protein-drug lifetimes in human cells by F NMR spectroscopy
The cellular environment is a complex and crowded space, with organelles, compartments and multitudes of molecules engaged...
COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript
COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript
COLMARvista is presented as a new, highly versatile software for the easy and intuitive processing and visual inspection o...
Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the determination of protein structure. The k...
The SOFAST-HMBC-HMQC experiment for pairing geminal methyl groups in valine and leucine side-chains
The SOFAST-HMBC-HMQC experiment for pairing geminal methyl groups in valine and leucine side-chains
Methyl groups are essential probes for characterising interactions and dynamics in large proteins. HN-based triple-resonan...
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement
Paramagnetic relaxation enhancement (PRE) is widely used in biomolecular NMR spectroscopy to obtain long-range distance an...
Estimating cross-relaxation rates between methyl and neighboring labile proton spins in high molecular weight proteins
Estimating cross-relaxation rates between methyl and neighboring labile proton spins in high molecular weight proteins
We show that water saturation leads to deleterious losses in sensitivity of methyl signals in selectively methyl-[13CH3]-l...
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-19F probe in nucleic acids
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-19F probe in nucleic acids
Solution NMR spectroscopy is a particularly powerful technique for characterizing the functional dynamics of biomolecules,...
Transverse relaxation optimized spectroscopy of NH2 groups in glutamine and asparagine side chains of proteins
Transverse relaxation optimized spectroscopy of NH2 groups in glutamine and asparagine side chains of proteins
A transverse relaxation optimized spectroscopy (TROSY) approach is described for the optimal detection of NH2 groups in as...
Optimising in-cell NMR acquisition for nucleic acids
Optimising in-cell NMR acquisition for nucleic acids
Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology and...
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Side chain isotope labelling is a powerful tool to study protein structure and interactions by NMR spectroscopy. 1H,13C la...
Pitfalls in measurements of R1 relaxation rates of protein backbone 15N nuclei
Pitfalls in measurements of R1 relaxation rates of protein backbone 15N nuclei
The dynamics of the backbone and side-chains of protein are routinely studied by interpreting experimentally determined 15...
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa wherea...
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, mater...
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium (2H) spin relaxation of 13CH2D methyl groups has been widely applied to investigate picosecond-to-nanosecond con...
Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight
Labeling of methyl groups: a streamlined protocol and guidance for the selection of 2H precursors based on molecular weight
A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium f...
19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
The recent application of 19F NMR in the study of biomolecular structure and dynamics has made it a potentially attractive...
Fluorine labelling for in situ 19F NMR in oriented systems
Fluorine labelling for in situ 19F NMR in oriented systems
The focus of this project is to take advantage of the large NMR chemical shift anisotropy of 19F to determine the orientat...
A comprehensive assessment of selective amino acid 15N-labeling in human embryonic kidney 293 cells for NMR spectroscopy
A comprehensive assessment of selective amino acid 15N-labeling in human embryonic kidney 293 cells for NMR spectroscopy
A large proportion of human proteins contain post-translational modifications that cannot be synthesized by prokaryotes. T...
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains...
Short and long range 2D 15N–15N NMR correlations among peptide groups by novel solid state dipolar mixing schemes
Short and long range 2D 15N–15N NMR correlations among peptide groups by novel solid state dipolar mixing schemes
A recently developed homonuclear dipolar recoupling scheme, Adiabatic Linearly FREquency Swept reCOupling (AL FRESCO), was...
Beyond slow two-state protein conformational exchange using CEST: applications to three-state protein interconversion on the millisecond timescale
Beyond slow two-state protein conformational exchange using CEST: applications to three-state protein interconversion on the millisecond timescale
Although NMR spectroscopy is routinely used to study the conformational dynamics of biomolecules, robust analyses of the d...
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