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Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density
Biofilm formation is the most effective pathway for electron transfer to anodes in bioelectrochemical systems. However, th...
Establishment of a cell-free multi-enzyme cascade for the synthesis of UDP-GalNAc
Volume 89, 25 November 2025, Pages 20-28Author links open overlay panel, , , , Highlights•Multi-enzyme cascade developed f...
Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds
This study evaluated single-cell protein production from PHA-rich mixed microbial cultures obtained from fermentation and ...
Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture
Volume 89, 25 November 2025, Pages 40-50Author links open overlay panel, , , , , Highlights•PHA-producers enrichment in CS...
On-demand insulin manufacturing using cell-free systems with an "on-column" conversion approach
Volume 89, 25 November 2025, Pages 51-59Author links open overlay panel, , , , , , , Highlights•Successful "On-Column" Con...
Phenolic compounds fingerprints show distinct ligninolytic bacteria behaviours during technical lignins conversion
Lignins, one of the main components of plant cell wall, and by-products of certain industries (paper and wood industries,…...
Hac1p-based inverse secretory pathway engineering (Hi-SPE) of Pichia pastoris for improved glucose oxidase production
Volume 89, 25 November 2025, Pages 82-90Author links open overlay panel, , , , , , Highlights•Hi-SPE narrows target genes ...
Model-based engineering of Bacillus methanolicus towards de novo polyamine bioproduction from methanol
Volume 89, 25 November 2025, Pages 91-104Author links open overlay panel, , , , , , Highlights•First proof-of-concept mode...
Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae
CRISPR interference (CRISPRi) technologies have revolutionized bioengineering by providing precise tools for gene expressi...
Using control bias to identify initial targets for bioproduction improvement
Sensitivity analysis of bioprocess metabolic reaction networks analysis allows the prediction of system parameters such as...
Biology-aware machine learning for culture medium optimization
Volume 89, 25 November 2025, Pages 141-151Author links open overlay panel, Highlights•Build an ML-guided platform for opti...
Future food court in an urban shopping center can supply feedstocks for microbial protein production
Volume 89, 25 November 2025, Pages 152-162Author links open overlay panel, , , , , Highlights•Food and beverage side strea...
Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation
Transparent mutant fish have been obtained from small-sized fish (medaka, zebrafish, and killifish), and are often used as...
tiRNA: An efficient and controllable gene silencing technology via translation inhibition
RNA-targeted therapies have emerged as a revolutionary breakthrough in biomedicine recently, offering unprecedented precis...
Improving PHB production in cyanobacteria: Modeling the optimal light regime for growth
Volume 89, 25 November 2025, Pages 73-81Author links open overlay panel, , , , , , , , , , , , , , , Highlights•Optimizati...
PHB in cyanobacteria: analyzing production through images processing and FT-IR techniques.
Volume 89, 25 November 2025, Pages 119-129Author links open overlay panel, , , , , , , , , , , , , , Highlights•Optimizati...
Sprinkling in extra validation for high-value PTMs and therapeutic Abs with MILKSHAKE Western blots and Sundae ELISAs
Volume 89, 25 November 2025, Pages 11-19Author links open overlay panel, , , , , , , , , , , , , , , , Highlights•Post tra...
Viability assessment and biomass valorization of recombinant Komagataella phaffii
Komagataella phaffii has become a key player in biotechnology used for the production of heterologous proteins with numero...
Public perception of new plant breeding techniques for sustainable production of feed and food in the Czech Republic
Volume 90, 25 December 2025, Pages 13-19Author links open overlay panel, , Highlights•Perception of new plant breeding tec...
Development and performance evaluation of a novel, fully automated, high-throughput GAD65 autoantibody assay on the Phadia platform
Volume 542, August 2025, 113885Author links open overlay panel, , , , , , , AbstractObjectivesIn clinics, diabetes is dete...
Characterization of antigen B subunit 2 (AgB2) gene polymorphism in sheep isolates of Echinococcus granulosus sensu lato and effect on serologic response
Echinococcus granulosus sensu lato is one of the most important zoonotic parasites causing Cystic Echinococcosis (CE) in h...
Development of multiplexed flow based assay for simultaneous identification and estimation of meningococcal capsular polysaccharide serogroups A, C, W, Y and X for conjugate vaccine manufacturing
Meningococcal meningitis is a bacterial form of meningitis caused by the gram-negative bacteria N. meningitidis. Meningiti...
Development of vectors for reformatting scFv fragments derived from phage display libraries into native IgG1 structures for in vivo imaging and therapeutic applications
The development of therapeutic monoclonal antibodies is continually expanding, and these immunotherapeutics find broad app...
Development of an ELISA strategy for the serological diagnosis of farmer's lung disease
Hypersensitivity pneumonitis (HP) is a family of interstitial lung diseases that occur in susceptible individuals followin...
Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications
Diarrhea is a major contributor to morbidity and mortality in children under five years of age, responsible for an estimat...
Development of microfluidic ELISA for measuring humoral responses to clostridial antigens in vaccinated cattle
Clostridial diseases significantly threaten livestock health, particularly in cattle, underscoring the need for effective ...
An unexpected bioanalytical challenge caused by positive control antibodies in a clinical immunogenicity assay – A simple solution and broadly applicable recommendations
Anti-drug antibody (ADA) assays are important in development of recombinant-protein therapeutic drugs. Appropriate detecti...
Development of a chimeric monoclonal antibody as a potential diagnostic reference in PR3-ANCA detection
Anti-neutrophil cytoplasmic antibodies (ANCA) are critical serological markers for ANCA-associated vasculitis (AAV), prima...
A high-throughput assay using dysfunctional T cells for phenotypic screening immune checkpoint modulators
T cells are essential components of the adaptive immune system that can recognize and eliminate pathogens or cancer cells....
An in-silico-designed multiepitope vaccine candidate can efficiently protect mice against pathogenic species of Shigella
Pathogenic species of Shigella cause a syndrome called shigellosis. Shigella flexneri (S. flexneri), Shigella boydii (S. b...
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