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Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays

In virology-related studies, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is routine to inac...

Journal Of Virological Methods

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Development and optimisation of influenza C and influenza D pseudotyped viruses

To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (P...

Journal Of Virological Methods

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Development and analytical validation of a quantitative PCR assay for the detection of Magellanic penguin herpesvirus 1

Free-living birds face various health risks due to viruses, such as avian influenza, West Nile Virus, and paramyxoviruses ...

Journal Of Virological Methods

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Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses

Since its emergence in 1996, highly pathogenic avian influenza (HPAI) viruses of the A/Goose/Guangdong/1/96 lineage have d...

Journal Of Virological Methods

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Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany

Apple rubbery wood disease is globally distributed and affects primarily apples and pears. A characteristic symptom of the...

Journal Of Virological Methods

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Curcumin inhibits HIV-1 by modulating FOXP3 and suppressing CCR5 via PI3K/AKT and JAK/STAT pathways

Acquired Immune Deficiency Syndrome (AIDS) is caused by infection with the human immunodeficiency virus (HIV). The viral a...

Journal Of Virological Methods

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In vitro modeling of human dorsal root ganglion neurons for GCaMP6-based calcium imaging of sensory responses to HSV-1 infection

Dorsal root ganglion (DRG) neurons, located in the dorsal root of spinal nerves, serve as critical relays for transmitting...

Journal Of Virological Methods

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Potential of recombinant avian adeno-associated virus as a viral vector for CRISPR/Cas9 delivery to avian cells

While genome editing has been established in chickens, where cultured primordial germ cell (PGC) systems are available, th...

Journal Of Virological Methods

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Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer

Persistent infection with high-risk human papillomavirus (hrHPV) causes almost all cases of cervical cancer. Despite the s...

Journal Of Virological Methods

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Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)

Tilapia Lake Virus (TiLV) is a newly emerging, highly infectious pathogen in aquaculture. It has been reported to affect t...

Journal Of Virological Methods

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Evaluation of dead-end hollowfiber ultrafilter options for enumerating somatic and F+ coliphage in ambient waters and wastewater

The discharge of improperly treated wastewater can contaminate receiving waterbodies. Within these waterbodies, exposure t...

Journal Of Virological Methods

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Development of a quadruple qPCR assay for simultaneous detection of four common bovine pathogens

Bovines play a crucial role in global agriculture and livestock production. However, outbreaks of infectious diseases in c...

Journal Of Virological Methods

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Evaluating the effectiveness of a novel environmental decontamination system utilizing low-energy hyper-charged photoelectrons against coronavirus

The COVID-19 pandemic had profound economic and social effects across the globe. The present study evaluated the virus att...

Journal Of Virological Methods

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Duplex droplet digital PCR enables simultaneous quantification of algal giant virus DSLLAV1 and virophage DSLV8 in natural and laboratory samples

Virophages are small double-stranded DNA (dsDNA) viruses that parasitize the replication machinery of giant viruses, addin...

Journal Of Virological Methods

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Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania

BackgroundCytomegalovirus (CMV) remains a significant infection in neonates and its early detection can aid with further t...

Journal Of Virological Methods

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Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces

Arboviruses are transmitted to humans and animals by arthropods and can be fatal. Dengue fever remains a major mosquito-bo...

Journal Of Virological Methods

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Evaluation of two IgG-scFv bispecific antibodies for neutralizing Omicron variants of SARS-CoV-2

Melissa García-Vega: Validation, Methodology, Formal analysis. Verónica Mata-Haro: Writing – review & editing, Validat...

Journal Of Virological Methods

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Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples

The global emergence of SARS-CoV-2 has highlighted the need for rapid, sensitive, and affordable diagnostic tools, not onl...

Journal Of Virological Methods

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A dual-target real-time PCR for proactive detection of Mpox variants

Monkeypox virus (MPXV) is a zoonotic infection that can be spread human-to-human (Lim et al., 2023). It has two main clade...

Journal Of Virological Methods

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Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP

Luis Galipienso: Supervision, Investigation, Formal analysis, Conceptualization. Luis Rubio: Writing – review & editin...

Journal Of Virological Methods

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Comparison of direct RNA sequencing of Orthoavulavirus javaense using two different chemistries on the MinION platform