Molecular virulotyping and advancing the detection of using a CRISPR-Cas12a-based diagnostic tool

Amoghavarsha C, Pramesh D, Naik GR et al (2022a) Morpho-molecular diversity and avirulence genes distribution among the diverse isolates of Magnaporthe oryzae from Southern India. J Appl Microbiol 132:1275–1290

Article  CAS  PubMed  Google Scholar 

Amoghavarsha C, Pramesh D, Sridhara S et al (2022b) Spatial distribution and identification of potential risk regions to rice blast disease in different rice ecosystems of Karnataka. Sci Rep 12:7403

Article  CAS  PubMed  PubMed Central  Google Scholar 

Aravind K, Rajeswari B, Babu TK et al (2024) A comprehensive survey and assessment of rice blast disease incidence from major rice growing districts of Telangana State, India. J Experimental Agric Int 46:484–491

Article  Google Scholar 

Baudin M, Le Naour-Vernet M, Gladieux P et al (2024) Pyricularia oryzae: lab star and field scourge. Mol Plant Pathol 25:e13449

Article  PubMed  PubMed Central  Google Scholar 

Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

Article  CAS  Google Scholar 

Chiang K-S, Liu HI, Bock C (2017) A discussion on disease severity index values. Part I: warning on inherent errors and suggestions to maximise accuracy. Ann Appl Biol 171:139–154

Article  Google Scholar 

Chittaragi A, Pramesh D, Naik GR et al (2022) Multilocus sequence analysis and identification of mating-type idiomorphs distribution in Magnaporthe oryzae population of Karnataka state of India. J Appl Microbiol 132:4413–4429

Article  CAS  PubMed  Google Scholar 

Chung H, Goh J, Han S-S et al (2020) Comparative pathogenicity and host ranges of Magnaporthe oryzae and related species. Plant Pathol J 36:305

Article  CAS  PubMed  PubMed Central  Google Scholar 

Diagne D, Adreit H, Milazzo J et al (2024) Population structure and pathogenicity tests of Pyricularia oryzae on wild and cultivated rice in Mali. Plant Pathol

Du Y, Yang J, Qi Z et al (2024) Rapid and visual detection of Pyricularia oryzae using coupled recombinase polymerase amplification-lateral flow dipstick assay. Plant Dis

Emani R, Prasad MS, VidyaSagar B et al (2024) Disentangling the genetic diversity and population structure of Magnaporthe oryzae from Telangana state of Southren Peninsular India. Physiol Mol Plant Pathol 129:102184

Article  CAS  Google Scholar 

Fan X, Zhang P, Batool W et al (2023) Contribution of the tyrosinase (MoTyr) to melanin synthesis, conidiogenesis, appressorium development, and pathogenicity in Magnaporthe oryzae. J Fungi 9:311

Article  CAS  Google Scholar 

Fernandez J, Wilson RA (2011) The sugar sensor, trehalose-6-phosphate synthase (Tps1), regulates primary and secondary metabolism during infection by the rice blast fungus: will Magnaporthe oryzae’s sweet tooth become its Achilles’ heel? Mycology 2:46–53

Article  CAS  Google Scholar 

Gladieux P, Condon B, Ravel S et al (2018) Gene flow between divergent cereal-and grass-specific lineages of the rice blast fungus Magnaporthe oryzae. mBio 9:10–1128

Article  Google Scholar 

Grund E, Gagey MJ, Toquin V et al (2013) Functional analysis of the Mps1 MAP kinase pathway in the rice blast fungus Magnaporthe orysae. In: 27th Fungal Genetics Conference,. p p-179

Guo Y, Xia H, Dai T et al (2023a) CRISPR/Cas12a-based approaches for efficient and accurate detection of Phytophthora ramorum. Front Cell Infect Microbiol 13:1218105

Article  CAS  PubMed  PubMed Central  Google Scholar 

Guo Y, Xia H, Dai T, Liu T (2023b) RPA-CRISPR/Cas12a mediated isothermal amplification for visual detection of Phytophthora Sojae. Front Cell Infect Microbiol 13:1208837

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gupta L, Vermani M, Kaur Ahluwalia S, Vijayaraghavan P (2021) Molecular virulence determinants of Magnaporthe oryzae: disease pathogenesis and recent interventions for disease management in rice plant. Mycology 12:174–187

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hariharan G, Prasannath K (2021) Recent advances in molecular diagnostics of fungal plant pathogens: a mini review. Front Cell Infect Microbiol 10:600234

Article  PubMed  PubMed Central  Google Scholar 

Huang J, Cook DE (2022) CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae. STAR Protoc 3:101072

Article  CAS  PubMed  Google Scholar 

Huang L, Li X, Li Y et al (2019) Endosidin2-14 targets the exocyst complex in plants and fungal pathogens to inhibit exocytosis. Plant Physiol 180:1756–1770

Article  PubMed  PubMed Central  Google Scholar 

Ikeda K, Uchihashi K, Okuda I et al (2024) Specific detection of Pyricularia oryzae pathotype Triticum using qPCR and LAMP methods. J Gen Plant Pathol 90:82–94

Article  CAS  Google Scholar 

Jagadeesh D, Rakshith D, Sharvani KA, Devaki NS (2022) Evaluation of virulence and race identification of rice-blast fungus Pyricularia oryzae isolated from different districts of Karnataka, India. Archives Phytopathol Plant Prot 55:1265–1282

Article  CAS  Google Scholar 

Jia Y, Zhou E, Lee S, Bianco T (2016) Coevolutionary dynamics of rice blast resistance gene Pi-ta and Magnaporthe oryzae avirulence gene AVR-Pita 1. Phytopathology 106:676–683

Article  CAS  PubMed  Google Scholar 

Kim Y, Park S-Y, Kim D et al (2013) Genome-scale analysis of ABC transporter genes and characterization of the ABCC type transporter genes in Magnaporthe oryzae. Genomics 101:354–361

Article  CAS  PubMed  Google Scholar 

Kumar S, Kashyap PL, Mahapatra S et al (2021) New and emerging technologies for detecting Magnaporthe oryzae causing blast disease in crop plants. Crop Prot 143:105473

Article  CAS  Google Scholar 

Lan B, Yang Y, Guo L et al (2020) Among-Population genetic diversity of rice blast fungus based on fingerprinting of Virulence-related genes. Physiol Mol Plant Pathol 112:101554

Article  CAS  Google Scholar 

Li L, Zhang SY, Zhang C-Q (2019) Establishment of a rapid detection method for rice blast fungus based on one-step loop-mediated isothermal amplification (LAMP). Plant Dis 103:1967–1973

Article  CAS  PubMed  Google Scholar 

Longya A, Talumphai S, Jantasuriyarat C (2020) Morphological characterization and genetic diversity of rice blast fungus, Pyricularia oryzae, from Thailand using ISSR and SRAP markers. J Fungi 6:38

Article  CAS  Google Scholar 

Mao L, Ying J, Selekon B et al (2022) Development and characterization of recombinase-based isothermal amplification assays (RPA/RAA) for the rapid detection of Monkeypox virus. Viruses 14:2112

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mentlak TA, Kombrink A, Shinya T et al (2012) Effector-mediated suppression of chitin-triggered immunity by Magnaporthe oryzae is necessary for rice blast disease. Plant Cell 24:322–335

Article  CAS  PubMed  PubMed Central  Google Scholar 

Palanna KB, Vinaykumar HD, Prasanna SK et al (2023) Exploring the diversity of virulence genes in the Magnaporthe population infecting millets and rice in India. Front Plant Sci 14:1131315

Article  CAS  PubMed  PubMed Central  Google Scholar 

Patel R, Mitra B, Vinchurkar M et al (2022) A review of recent advances in plant-pathogen detection systems. Heliyon 8

Prasannakumar MK, Parivallal PB, Pramesh D et al (2021) LAMP-based foldable microdevice platform for the rapid detection of Magnaporthe oryzae and Sarocladium oryzae in rice seed. Sci Rep 11:178

Article  CAS  PubMed  PubMed Central  Google Scholar 

Qi Z, Pan X, Du Y et al (2021) Pathogenicity and population structure analysis of Pyricularia oryzae in different districts of Jiangsu Province, China. Plant Pathol 70:449–458

Article  CAS  Google Scholar 

Qi Z, Ju F, Guo Y et al (2024) A rapid, equipment-free method for detecting avirulence genes of Pyricularia oryzae using a lateral flow strip-based RPA assay. Plant Dis

Rajput N, Younas MU, Qasim M et al (2025) Understanding rice blast: investigating biotechnological methods to speed up the development of robust rice cultivars. Genet Resour Crop Evol 72:1333–1352

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