Almeida CAS, Baggio SR, Mariutti LRB, Bragagnolo N (2020) One-step rapid extraction of phytosterols from vegetable oils. Food Res Int 130:108891

Article  PubMed  CAS  Google Scholar 

Anuradha B (2019) Steroids market report global forecast from 2025 to 2033. Market Research-Dataintelo. Accessed 13 April 2025. https://dataintelo.com/report/steroids-market-report

Bai G, Ma C-G, Chen X-W (2021) Effect of unsaturation of free fatty acids and phytosterols on the formation of esterified phytosterols during deodorization of corn oil. J Sci Food Agric 101(7):2736–2743

Article  PubMed  CAS  Google Scholar 

Cao D, Zhu X, Zhang J, Wang X (2023) A combined strategy to enhance phytosterols biotransformation to 22-hydroxy-23,24-bisnorchol-4-ene-3-one by Mycobacterium neoaurum. Process Biochem 128:94–97

Article  CAS  Google Scholar 

Chen S, Bi Y, Wang X, Yang G, Zhang H (2017) The analysis of sterols and sterol esters of corn germ oil by GC-MS/FID. J Chin Cereals Oils Association 32(5):131–137

Google Scholar 

Gao XQ, Feng JX, Wang XD, Hua Q, Wei DZ (2015) Enhanced steroid metabolites production by resting cell phytosterol bioconversion. Chem Biochem Eng Q 29(4):567–573

Article  CAS  Google Scholar 

Han S, Liu X, He B, Zhai X, Yuan C, Li Y, Lin W, Wang H, Zhang B (2024) Efficient production of 9,22-dihydroxy-23,24-bisnorchol-4-ene-3-one from phytosterols by modifying multiple genes in Mycobacterium fortuitum. Int J Mol Sci 25(7):3579

Article  PubMed  PubMed Central  CAS  Google Scholar 

Jiang S, Zhao J, Yang Y, Hu J, Cui L (2008) Study on transformation of phytosterol into androst-4-ene-3,17-dione in two-phase systems. Food Ferment Industries 34(6):46–48

CAS  Google Scholar 

Kuypers MMM, Marchant HK, Kartal B (2018) The microbial nitrogen-cycling network. Nat Rev Microbiol 16(5):263–276

Article  PubMed  CAS  Google Scholar 

Li R, Guo S, Wang D, Yang T, Li W, Wang J, Huang L, Zhang X, Dai Z (2025) Elucidation of the plant progesterone biosynthetic pathway and its application in a yeast cell factory. Metab Eng 90:197–208

Article  PubMed  CAS  Google Scholar 

Liu X, Zhu P, Jiang R, Wu L, Feng X, Li S, Xu H (2017) Enhancement of Welan gum production in Sphingomonas Sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene. Carbohydr Polym 156:135–142

Article  PubMed  CAS  Google Scholar 

Liu Y-J, Ji W-t, Song L, Tao X-Y, Zhao M, Gao B, Meng H, Wang F-Q, Wei D-Z (2022) Transformation of phytosterols into pregnatetraenedione by a combined microbial and chemical process. Green Chem 24(9):3759–3771

Article  CAS  Google Scholar 

Liu X, He B, Zhang J, Yuan C, Han S, Du G, Shi J, Sun J, Zhang B (2023) Phytosterol conversion into C9 non-hydroxylated derivatives through gene regulation in Mycobacterium fortuitum. Appl Microbiol Biotechnol 107(24):7635–7646

Article  PubMed  CAS  Google Scholar 

Malaviya A, Gomes J (2008) Androstenedione production by biotransformation of phytosterols. Bioresour Technol 99(15):6725–6737

Article  PubMed  CAS  Google Scholar 

Mancilla RA, Little C, Amoroso A (2018) Efficient bioconversion of high concentration phytosterol microdispersion to 4-androstene-3,17-dione (AD) by Mycobacterium Sp. B3805. Appl Biochem Biotechnol 185(2):494–506

Article  PubMed  CAS  Google Scholar 

Shtratnikova VY, Schelkunov MI, Dovbnya DV, Bragin EY, Donova MV (2017) Effect of methyl-β-cyclodextrin on gene expression in microbial conversion of phytosterol. Appl Microbiol Biotechnol 101(11):4659–4667

Article  PubMed  CAS  Google Scholar 

Sonia M-C, Esther B, José-Luis B, Marta R-S (2017) Scale-Up of phytosterols bioconversion into Androstenedione. Methods Mol Biology (Clifton N J) 1645:199–210

Article  Google Scholar 

Soto Laveaga G (2005) Uncommon trajectories: steroid hormones, Mexican peasants, and the search for a wild Yam. Stud History Philos Sci Part C: Stud History Philos Biol Biomedical Sci 36(4):743–760

Google Scholar 

Stefanov S, Yankov D, Beschkov V (2006) Biotransformation of phytosterols to Androstenedione in two phase water-oil systems. Chem Biochem Eng Q 20(4):421–427

CAS  Google Scholar 

Su L, Xu S, Shen Y, Xia M, Ren X, Wang L, Shang Z, Wang M (2020) The sterol carrier hydroxypropyl-β-cyclodextrin enhances the metabolism of phytosterols by Mycobacterium neoaurum. Appl Environ Microbiol 86(15):e00441–e00420

Article  PubMed  PubMed Central  CAS  Google Scholar 

Sun W-J, Wang L, Liu H-H, Liu Y-J, Ren Y-H, Wang F-Q, Wei D-Z (2019) Characterization and engineering control of the effects of reactive oxygen species on the conversion of sterols to steroid synthons in Mycobacterium neoaurum. Metab Eng 56:97–110

Article  PubMed  CAS  Google Scholar 

Wang X, Chen R, Wu Y, Wang D, Wei D (2020) Nitrate metabolism decreases the steroidal alcohol byproduct compared with ammonium in biotransformation of phytosterol to Androstenedione by Mycobacterium neoaurum. Appl Biochem Biotechnol 190(4):1553–1560

Article  PubMed  CAS  Google Scholar 

Wang D, Zhang J, Cao D-D, Wang X, Wei D (2021) Identification and in situ removal of an inhibitory intermediate to develop an efficient phytosterol bioconversion process using a cyclodextrin-resting cell system. RSC Adv 11(40):24787–24793

Article  PubMed  PubMed Central  CAS  Google Scholar 

Wang X-X, Ke X, Liu Z-Q, Zheng Y-G (2022) Rational development of mycobacteria cell factory for advancing the steroid biomanufacturing. World J Microbiol Biotechnol 38(11):191–191

Article  PubMed  PubMed Central  CAS  Google Scholar 

Xia X, Ren M, He W-S, Jia C, Zhang X (2022) The Preparation of phytosteryl succinyl sucrose esters and improvement of their water solubility and emulsifying properties. Food Chem 373:131501

Article  PubMed  CAS  Google Scholar 

Xin L, Tian C, Fei P, Shikui S, Jingpeng Y, Njimeli SD, Xiyao C, Yongqi H, Yijun H, Zhengding S (2021) Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria. Microb Cell Fact 20(1):158

Article  Google Scholar 

Xiong L-B, Liu H-H, Zhao M, Liu Y-J, Song L, Xie Z-Y, Xu Y-X, Wang F-Q, Wei D-Z (2020) Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of KasB in the cell wall synthesis of Mycobacterium neoaurum. Microb Cell Fact 19(1):80

Article  PubMed  PubMed Central  CAS  Google Scholar 

Xu H, Su Z, Li W, Deng Y, He Z-G (2021) MmbR, a master transcription regulator that controls fatty acid β-oxidation genes in Mycolicibacterium smegmatis. Environ Microbiol 23(2):1096–1114

Article  PubMed  CAS  Google Scholar 

Yang H, Yan F, Wu D, Huo M, Li J, Cao Y, Jiang Y (2010) Recovery of phytosterols from waste residue of soybean oil deodorizer distillate. Bioresour Technol 101(5):1471–1476

Article  PubMed  CAS  Google Scholar 

Yang R, Xue L, Zhang L, Wang X, Qi X, Jiang J, Yu L, Wang X, Zhang W, Zhang Q, Li P (2019) Phytosterol contents of edible oils and their contributions to estimated phytosterol intake in the Chinese diet. Foods 8(8):334

Article  PubMed  PubMed Central  CAS  Google Scholar 

Yao S, Zhang Q, Dou W, Peng D, Bi Y, chen J (2023) Isolation and purification of sterol esters from soybean germ. Food Res Dev 44(4):72–78

CAS  Google Scholar 

Yuan J-J, Guan Y-X, Yao S-J (2017) Evaluation of biocompatible ionic liquids for their application in phytosterols bioconversion by Mycobacterium Sp. resting cells. ACS Sustain Chem Eng 5(11):10702–10709

Article  CAS  Google Scholar 

Zhang X, Jiang D, Zhang H, Wang Y, Zhang Z, Lu C, Zhang Q (2021) Enhancement of the biohydrogen production performance from mixed substrate by photo-fermentation: effects of initial pH and inoculation volume ratio. Bioresour Technol 319:124153

Article  PubMed  CAS  Google Scholar