Serum metabolomics profiling by proton nuclear magnetic resonance spectrometry of the response to single oral macronutrient challenges in women with polycystic ovary syndrome (PCOS) compared with male and female controls

Escobar-Morreale HF. Polycystic ovary syndrome: definition, aetiology, diagnosis and treatment. Nat Rev Endocrinol. 2018;14:270–84.

Article  PubMed  Google Scholar 

Borruel S, Fernandez-Duran E, Alpanes M, Marti D, Alvarez-Blasco F, Luque-Ramirez M, Escobar-Morreale HF. Global adiposity and thickness of intraperitoneal and mesenteric adipose tissue depots are increased in women with polycystic ovary syndrome (PCOS). J Clin Endocrinol Metab. 2013;98:1254–63.

Article  CAS  PubMed  Google Scholar 

Alvarez-Blasco F, Botella-Carretero JI, San Millan JL, Escobar-Morreale HF. Prevalence and characteristics of the polycystic ovary syndrome in overweight and obese women. Arch Intern Med. 2006;166:2081–6.

Article  PubMed  Google Scholar 

Ortiz-Flores AE, Luque-Ramirez M, Fernandez-Duran E, Alvarez-Blasco F, Escobar-Morreale HF. Diagnosis of disorders of glucose tolerance in women with polycystic ovary syndrome (PCOS) at a tertiary care center: fasting plasma glucose or oral glucose tolerance test? Metabolism. 2019;93:86–92.

Article  CAS  PubMed  Google Scholar 

Escobar-Morreale HF, San Millan JL. Abdominal adiposity and the polycystic ovary syndrome. Trends Endocrinol Metab. 2007;18:266–72.

Article  CAS  PubMed  Google Scholar 

Diamanti-Kandarakis E, Dunaif A. Insulin resistance and the polycystic ovary syndrome revisited: an update on mechanisms and implications. Endocr Rev. 2012;33:981–1030.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Escobar-Morreale HF, Samino S, Insenser M, Vinaixa M, Luque-Ramirez M, Lasuncion MA, Correig X. Metabolic heterogeneity in polycystic ovary syndrome is determined by obesity: plasma metabolomic approach using GC-MS. Clin Chem. 2012;58:999–1009.

Article  CAS  PubMed  Google Scholar 

Escobar-Morreale HF, Alvarez-Blasco F, Botella-Carretero JI, Luque-Ramirez M. The striking similarities in the metabolic associations of female androgen excess and male androgen deficiency. Hum Reprod. 2014;29:2083–91.

Article  CAS  PubMed  Google Scholar 

Luque-Ramirez M, Martinez-Garcia MA, Montes-Nieto R, Fernandez-Duran E, Insenser M, Alpanes M, Escobar-Morreale HF. Sexual dimorphism in adipose tissue function as evidenced by circulating adipokine concentrations in the fasting state and after an oral glucose challenge. Hum Reprod. 2013;28:1908–18.

Article  CAS  PubMed  Google Scholar 

Montes-Nieto R, Insenser M, Murri M, Fernandez-Duran E, Ojeda-Ojeda M, Martinez-Garcia MA, Luque-Ramirez M, Escobar-Morreale HF. Plasma thiobarbituric acid reactive substances (TBARS) in young adults: obesity increases fasting levels only in men whereas glucose ingestion, and not protein or lipid intake, increases postprandial concentrations regardless of sex and obesity. Mol Nutr Food Res. 2017;61:1700425.

Article  Google Scholar 

Mauvais-Jarvis F. Sex differences in metabolic homeostasis, diabetes, and obesity. Biol Sex Differ. 2015;6:14.

Article  PubMed  PubMed Central  Google Scholar 

Martinez-Garcia MA, Montes-Nieto R, Fernandez-Duran E, Insenser M, Luque-Ramirez M, Escobar-Morreale HF. Evidence for masculinization of adipokine gene expression in visceral and subcutaneous adipose tissue of obese women with polycystic ovary syndrome (PCOS). J Clin Endocrinol Metab. 2013;98:E388–96.

Article  CAS  PubMed  Google Scholar 

Murri M, Insenser M, Fernandez-Duran E, San-Millan JL, Luque-Ramirez M, Escobar-Morreale HF. Non-targeted profiling of circulating microRNAs in women with polycystic ovary syndrome (PCOS): effects of obesity and sex hormones. Metabolism. 2018;86:49–60.

Article  CAS  PubMed  Google Scholar 

Montes-Nieto R, Insenser M, Martinez-Garcia MA, Escobar-Morreale HF. A nontargeted proteomic study of the influence of androgen excess on human visceral and subcutaneous adipose tissue proteomes. J Clin Endocrinol Metab. 2013;98:E576–85.

Article  CAS  PubMed  Google Scholar 

Insenser M, Montes-Nieto R, Vilarrasa N, Lecube A, Simo R, Vendrell J, Escobar-Morreale HF. A nontargeted proteomic approach to the study of visceral and subcutaneous adipose tissue in human obesity. Mol Cell Endocrinol. 2012;363:10–9.

Article  CAS  PubMed  Google Scholar 

Escobar-Morreale HF, Martínez-García MÁ, Insenser M, Cañellas N, Correig X, Luque-Ramírez M. Serum metabolomics profiling by proton nuclear magnetic resonance reveals sexual dimorphism and masculinization of intermediate metabolism in women with polycystic ovary syndrome (PCOS). Biol Sex Diff. 2023;14:21.

Article  CAS  Google Scholar 

Martinez-Garcia MA, Moncayo S, Insenser M, Alvarez-Blasco F, Luque-Ramirez M, Escobar-Morreale HF. Metabolic cytokines at fasting and during macronutrient challenges: influence of obesity, female androgen excess and sex. Nutrients. 2019;11:E2566.

Article  Google Scholar 

Martinez-Garcia MA, Moncayo S, Insenser M, Montes-Nieto R, Fernandez-Duran E, Alvarez-Blasco F, Luque-Ramirez M, Escobar-Morreale HF. Postprandial inflammatory responses after oral glucose, lipid and protein challenges: influence of obesity, sex and polycystic ovary syndrome. Clin Nutr. 2020;39:876–85.

Article  CAS  PubMed  Google Scholar 

Martinez-Garcia MA, Ojeda-Ojeda M, Rodriguez-Martin E, Insenser M, Moncayo S, Alvarez-Blasco F, Luque-Ramirez M, Escobar-Morreale HF. TLR2 and TLR4 surface and gene expression in white blood cells after fasting and oral glucose, lipid and protein challenges: influence of obesity and sex hormones. Biomolecules. 2020;10:E111.

Article  Google Scholar 

Martinez-Garcia MA, Moncayo S, Insenser M, Alvarez-Blasco F, Luque-Ramirez M, Escobar-Morreale HF. Postprandial responses of circulating energy homeostasis mediators to single macronutrient challenges: influence of obesity and sex hormones. Food Funct. 2021;12:1051–62.

Article  PubMed  Google Scholar 

Fuertes-Martin R, Moncayo S, Insenser M, Martinez-Garcia MA, Luque-Ramirez M, Grau NA, Blanchar XC, Escobar-Morreale HF. Glycoprotein A and B height-to-width ratios as obesity-independent novel biomarkers of low-grade chronic inflammation in women with polycystic ovary syndrome (PCOS). J Proteome Res. 2019;18:4038–45.

Article  CAS  PubMed  Google Scholar 

Moncayo S, Insenser M, Martinez-Garcia MA, Fuertes-Martin R, Amigo-Grau N, Alvarez-Blasco F, Luque-Ramirez M, Correig-Blanchar X, Escobar-Morreale HF. Acute-phase glycoprotein profile responses to different oral macronutrient challenges: influence of sex, functional hyperandrogenism and obesity. Clin Nutr. 2021;40:1241–6.

Article  CAS  PubMed  Google Scholar 

Insenser M, Moncayo S, Martinez-Garcia MA, Fernandez-Duran E, Samino S, Alvarez-Blasco F, Luque-Ramirez M, Escobar-Morreale HF. 2D diffusion-ordered (1) H-NMR spectroscopy lipidomic profiling after oral single macronutrient loads: influence of obesity, sex, and female androgen excess. Mol Nutr Food Res. 2020;64: e1900928.

Article  PubMed  Google Scholar 

Zawadzki JK, Dunaif A. Diagnostic criteria for polycystic ovary syndrome: towards a rational approach. In: Dunaif A, Givens JR, Haseltine FP, Merriam GR, editors. Polycystic ovary syndrome. Boston: Blackwell Scientific Publications; 1992. p. 377–84.

Google Scholar 

Hatch R, Rosenfield RL, Kim MH, Tredway D. Hirsutism: implications, etiology, and management. Am J Obstet Gynecol. 1981;140:815–30.

Article  CAS  PubMed  Google Scholar 

Luque-Ramirez M, Alvarez-Blasco F, Botella-Carretero JI, Sanchon R, San Millan JL, Escobar-Morreale HF. Increased body iron stores of obese women with polycystic ovary syndrome are a consequence of insulin resistance and hyperinsulinism and are not a result of reduced menstrual losses. Diabetes Care. 2007;30:2309–13.

Article  CAS  PubMed  Google Scholar 

Escobar-Morreale HF, Serrano-Gotarredona J, Varela C, García-Robles R, Sancho JM. Circulating leptin concentrations in women with hirsutism. Fertil Steril. 1997;68:898–906.

Article  CAS  PubMed  Google Scholar 

Luque-Ramirez M, Marti D, Fernandez-Duran E, Alpanes M, Alvarez-Blasco F, Escobar-Morreale HF. Office blood pressure, ambulatory blood pressure monitoring, and echocardiographic abnormalities in women with polycystic ovary syndrome: role of obesity and androgen excess. Hypertension. 2014;63:624–9.

Article  CAS  PubMed  Google Scholar 

Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and ß-cell function from fasting plasma insulin and glucose concentrations in man. Diabetologia. 1985;28:412–9.

Article  CAS  PubMed  Google Scholar 

Matsuda M, DeFronzo RA. Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp. Diabetes Care. 1999;22:1462–70.

Article  CAS  PubMed  Google Scholar 

Mazer NA. A novel spreadsheet method for calculating the free serum concentrations of testosterone, dihydrotestosterone, estradiol, estrone and cortisol: with illustrative examples from male and female populations. Steroids. 2009;74:512–9.

Article  CAS  PubMed  Google Scholar 

Pearce JT, Athersuch TJ, Ebbels TM, Lindon JC, Nicholson JK, Keun HC. Robust algorithms for automated chemical shift calibration of 1D 1H NMR spectra of blood serum. Anal Chem. 2008;80:7158–62.

Article  CAS  PubMed  Google Scholar 

Canueto D, Gomez J, Salek RM, Correig X, Canellas N. rDolphin: a GUI R package for proficient automatic profiling of 1D (1)H-NMR spectra of study datasets. Metabolomics. 2018;14:24.

Article  PubMed  Google Scholar 

Wishart DS, Jewison T, Guo AC, Wilson M, Knox C, Liu Y, Djoumbou Y, Mandal R, Aziat F, Dong E, Bouatra S, Sinelnikov I, Arndt D, Xia J, Liu P, Yallou F, Bjorndahl T, Perez-Pineiro R, Eisner R, Allen F, Neveu V, Greiner R, Scalbert A. HMDB 3.0—the human metabolome database in 2013. Nucleic Acids Res. 2013;41:D801–7.

Article  CAS  PubMed  Google Scholar 

Nicholson JK, Foxall PJ, Spraul M, Farrant RD, Lindon JC. 750 MHz 1H and 1H–13C NMR spectroscopy of human blood plasma. Anal Chem. 1995;67:793–811.

Article  CAS  PubMed 

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