Wolff J.A., Malone R.W., Williams P., Chong, W., Acsadi G., Jani, A., Felgner P.L. 1990. Direct gene transfer into mouse muscle in vivo. Science. 247, 1465‒1468.

Article  CAS  PubMed  Google Scholar 

Tang D.C., DeVit M., Johnston S.A. 1992. Genetic immunization is a simple method for eliciting an immune response. Nature. 356, 152‒154.

Article  CAS  PubMed  Google Scholar 

Ulmer J.B., Donnelly J.J., Parker S.E., Rhodes G.H., Felgner P.L., Dwarki V.J., Gromkowski S.H., Deck R.R., DeWitt C.M., Friedman A., Hawe L.A., Leander K.R., Martinez D., Perry H.C., Shiver J.W., Montgomery D.L., Liu M.A. 1993. Heterologous protection against influenza by injection of DNA encoding a viral protein. Science. 259, 1745‒1749.

Article  CAS  PubMed  Google Scholar 

Shafaati M., Saidijam M., Soleimani M., Hazrati F., Mirzaei R., Amirheidari B., Tanzadehpanah H.; Karampoor S., Kazemi S., Yavari B., Mahaki H., Safaei M., Rahbarizadeh F., Samadi P., Ahmadyousefi Y. 2022. A brief review on DNA vaccines in the era of COVID-19. Future Virol. 17, 49‒66.

Article  CAS  Google Scholar 

Mallapaty S. 2021. India’s DNA COVID vaccine is a first—more are coming. Nature. 597, 161‒162.

Article  CAS  PubMed  Google Scholar 

Momin T., Kansagra K., Patel H., Sharma S., Sharma B., Patel J., Mittal R., Sanmukhani J., Maithal K., Dey A, Chandra H., Rajanathan C.T., Pericherla H.P., Kumar P., Narkhede A., Parmar D. 2021. Safety and Immunogenicity of a DNA SARS-CoV-2 vaccine (ZyCoV-D): Results of an open-label, non-randomized phase I part of phase I/II clinical study by intradermal route in healthy subjects in India. EClinicalMedicine. 38, 101020.

Article  PubMed  PubMed Central  Google Scholar 

Maslow J. N., Kwon I., Kudchodkar S. B., Kane D., Tadesse A., Lee H., Tadesse A., Lee H., Park Y.K., Muthumani K., Roberts C.C. 2023. DNA vaccines for epidemic preparedness: SARS-CoV-2 and beyond. Vaccines (Basel). 11, 1016.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Baghban R., Ghasemian A., Mahmoodi S. 2023. Nucleic acid-based vaccine platforms against the coronavirus disease 19 (COVID-19). Arch. Microbiol. 205, 150.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jahanafrooz Z., Baradaran B., Mosafer J., Hashem-zaei M., Rezaei T., Mokhtarzadeh A., Hamblin M.R. 2020. Comparison of DNA and mRNA vaccines against cancer. Drug Discovery Today. 25, 552‒560.

Article  PubMed  Google Scholar 

Tang J., Li M., Zhao C., Shen D., Liu L., Zhang X., Wei L. 2022. Therapeutic DNA vaccines against HPV-related malignancies: promising leads from clinical trials. Viruses. 14, 239.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Robertson J., Ackland J., Holm A. 2007. Guidelines for assuring the quality and nonclinical safety evaluation of DNA vaccines. WHO Tech. Rep. Ser. 941, 57‒81. https://www.who.int/publications/m/item/annex-1-trs941-dna-vax

Sheets R., Kang H.N., Meyer H., Knezevic I. 2020. WHO informal consultation on the guidelines for evaluation of the quality, safety, and efficacy of DNA vaccines, Geneva, Switzerland, December 2019. NPJ Vaccines. 5, 52.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Beasley D.W. 2020. New international guidance on quality, safety and efficacy of DNA vaccines. NPJ Vaccines. 5, 53.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ghaffarifar F. 2018. Plasmid DNA vaccines: Where are we now? Drugs Today (Barc.). 54 (5), 315‒333.

Article  CAS  PubMed  Google Scholar 

Pagliari S., Dema B., Sanchez-Martinez A., Flores G.M.Z., Rollier C.S. 2023. DNA vaccines: History, molecular mechanisms and future perspectives. J. Mol. Biol. 453, 168297.

Article  Google Scholar 

Xia W., Bringmann P., McClary J., Jones P.P., Manzana W., Zhu Y., Wang S., Liu Y., Harvey S., Madlansacay M.R., McLean K., Rosser M.P., MacRobbie J., Olsen C.L., Cobb R.R. 2006. High levels of protein expression using different mammalian CMV promoters in several cell lines. Protein Expr. Purif. 45, 115‒124.

Article  CAS  PubMed  Google Scholar 

Johari Y.B., Scarrott J.M., Pohle T.H., Liu P., Mayer A., Brown A.J., James D.C. 2022. Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells. Biotechnol. J. 17, 2200062.

Article  CAS  Google Scholar 

Duan B., Cheng L., Gao Y., Yin F.X., Su G.H., Shen Q.Y., Liu K., Hu X., Liu X., Li G.P. 2012. Silencing of fat-1 transgene expression in sheep may result from hypermethylation of its driven cytomegalovirus (CMV) promoter. Theriogenology. 78, 793‒802.

Article  CAS  PubMed  Google Scholar 

Franck C.O., Fanslau L., Bistrovic Popov A., Tyagi P., Fruk L. 2021. Biopolymer-based carriers for DNA vaccine design. Angew. Chem. Int. Ed. Engl. 60, 13225‒13243.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Krinner S., Heitzer A., Asbach B., Wagner R. 2015. Interplay of promoter usage and intragenic CpG content: Impact on GFP reporter gene expression. Hum. Gene Ther. 26, 826‒840.

Article  CAS  PubMed  Google Scholar 

Dean D.A., Dean B.S., Muller S., Smith L.C. 1999. Sequence requirements for plasmid nuclear import. Exp. Cell Res. 253, 713‒722.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li H.S., Yong L.I.U., Li D.F., Zhang R.R., Tang H.L., Zhang Y.W., Huang W., Liu Y., Peng H., Xu J., Hong K., Shao Y.M. 2007. Enhancement of DNA vaccine-induced immune responses by a 72-bp element from SV40 enhancer. Chin. Med. J. (Engl.). 120, 496‒502.

Article  CAS  PubMed  Google Scholar 

Li L., Petrovsky N. 2016. Molecular mechanisms for enhanced DNA vaccine immunogenicity. Exp. Rev. Vaccines. 15, 313‒329.

Article  CAS  Google Scholar 

Sunita S.A., Singh Y., Shukla P. 2020. Computational tools for modern vaccine development. Hum. Vaccines Immunother. 16, 723‒735.

Article  CAS  Google Scholar 

Dobaño C., Sedegah M., Rogers W.O., Kumar S., Zheng H., Hoffman S.L., Doolan D.L. 2009. Plasmodium: mammalian codon optimization of malaria plasmid DNA vaccines enhances antibody responses but not T cell responses nor protective immunity. Exp. Parasitol. 122, 112‒123.

Article  PubMed  Google Scholar 

Varaldo P.B., Miyaji E.N., Vilar M.M., Campos A.S., Dias W.O., Armôa G.R., Tendler M., Leite L.C.C., McIntosh D. 2006. Mycobacterial codon optimization of the gene encoding the Sm14 antigen of Schistosoma mansoni in recombinant Mycobacterium bovis Bacille Calmette-Guérin enhances protein expression but not protection against cercarial challenge in mice. FEMS Immunol. Med. Microbiol. 48, 132‒139.

Article  CAS  PubMed  Google Scholar 

Staudacher J., Rebnegger C., Dohnal T., Landes N., Mattanovich D., Gasser B. 2022. Going beyond the limit: increasing global translation activity leads to increased productivity of recombinant secreted proteins in Pichia pastoris. Metab. Eng. 70, 181‒195.

Article  CAS  PubMed  Google Scholar 

Seymour B.J., Singh S., Certo H.M., Sommer K., Sather B.D., Khim S., Rawlings D.J. 2021. Effective, safe, and sustained correction of murine XLA using a UCOE-BTK promoter-based lentiviral vector. Mol. Ther. Methods Clin. Dev. 20, 635‒651.

Article  CAS  PubMed  PubMed Central  Google Scholar 

McCluskie M.J., Weeratna R.D., Davis H.L. 2000. The role of CpG in DNA vaccines. Springer Semin. Immunopathol. 22, 125‒132.

Article  CAS  PubMed  Google Scholar 

Williams J.A. 2013. Vector design for improved DNA vaccine efficacy, safety and production. Vaccines (Basel). 1, 225‒249.

Article  PubMed  PubMed Central  Google Scholar 

von Heijne G. 1998. Life and death of a signal peptide. Nature. 396, 111‒113.