Substrate Efficiency of Cy5-Modified Derivatives of Deoxyuridine and Deoxycytidine in the Rolling Circle Amplification

Oliveira B.B., Veigas B., Baptista P.V. 2021. Isothermal amplification of nucleic acids: the race for the next “gold standard”. Front. Sensors. 2, 752600. https://doi.org/10.3389/fsens.2021.752600

Ali M.M., Li F., Zhang Z., Zhang K., Kang D.K., Ankrum J.A., Le X.C., Zhao W. 2014. Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine. Chem. Soc. Rev. 43, 3324–3341.

Article  CAS  PubMed  Google Scholar 

Mujumdar R.B., Ernst L.A, Mujumdar S.R., Lewis C.J., Waggoner A.S. 1993. Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconjug. Chem. 4, 105–111.

Article  CAS  PubMed  Google Scholar 

Ren X., El-Sagheer A.H., Brown T. 2016. Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes. Nucleic Acids Res. 44, e79.

Article  PubMed  PubMed Central  Google Scholar 

Lapa S.A., Chirkova P.A., Surzhikov S.A., Kuznetsova V.E., Shershov V.E., and Chudinov A.V. 2024. A comparative behavior analysis of Cy5-pyrimidine nucleotides in rolling circle amplification. Russ. J. Bioorg. Chem. 50, 1351–1356. https://doi.org/10.1134/S1068162024040101

Article  CAS  Google Scholar 

Lapa S.A., Miftakhov R.A., Klochikhina E.S., Ammur Y.I., Blagodatskikh S.A., Shershov V.E., Zasedatelev A.S., Chudinov A.V. 2021. Development of мultiplex RT-PCR with immobilized primers for identification of infectious human pneumonia pathogens. Mol. Biol. 55, 828–838. https://doi.org/10.1134/S0026893321040063

Article  CAS  PubMed  PubMed Central  Google Scholar 

Spitsyn M.A., Kuznetsova V.E., Shershov V.E., Emelyanova M.A., Guseinov T.O., Lapa S.A., Nasedkina T.V., Zasedatelev A.S., Chudinov A.V. 2017. Synthetic route to novel zwitterionic pentamethine indocyanine fluorophores with various substitutions. Dyes Pigments. 147, 199–210.

Article  CAS  Google Scholar 

Zasedateleva O.A., Vasiliskov V.A., Surzhikov S.A., Kuznetsova V.E., Shershov V.E., Guseinov T.O., Smirnov I.P., Yurasov R.A., Spitsyn M.A., Chudinov A.V. 2018. dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase. Nucleic Acids Res. 46, e73.

Article  PubMed  PubMed Central  Google Scholar 

Shershov V.E., Lapa S.A., Levashova A.I., Shishkin I.Yu., Shtylev G.F., Shekalova E.Yu., Vasiliskov V.A., Zasedatelev A.S., Kuznetsova V.E., Chudinov A.V. 2023. Synthesis of fluorescent-labeled nucleotides for labeling of isothermal amplification products. Russ. J. Bioorg. Chem. 49, 1151–1158.

Article  CAS  Google Scholar 

Ramakers C., Ruijter J.M., Deprez R.H., Moorman A.F. 2003. Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339, 62–66.

Article  CAS  PubMed  Google Scholar 

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