Key Protein Design for Bone-Regenerative Engineering: From Core Biological Processes to Targeted Protein Discovery

Lopes D, Martins-Cruz C, Oliveira MB, Mano JF. Bone physiology as inspiration for tissue regenerative therapies. Biomaterials. 2018;185:240–75.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Koons GL, Diba M, Mikos AG. Materials design for bone-tissue engineering. Nat Rev Mater. 2020;5(8):584–603.

Article  CAS  Google Scholar 

Salhotra A, Shah HN, Levi B, Longaker MT. Mechanisms of bone development and repair. Nat Rev Mol Cell Biol. 2020;21(11):696–711.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang S, Ma B, Liu F, Duan J, Wang S, Qiu J, Li D, Sang Y, Liu C, Liu D, et al. Polylactic acid nanopillar array-driven osteogenic differentiation of human adipose-derived stem cells determined by pillar diameter. Nano Lett. 2018;18(4):2243–53.

Article  CAS  PubMed  Google Scholar 

Qiu J, Li J, Wang S, Ma B, Zhang S, Guo W, Zhang X, Tang W, Sang Y, Liu H. TiO2 nanorod array constructed nanotopography for regulation of mesenchymal stem cells fate and the realization of location-committed stem cell differentiation. Small. 2016;12(13):1770–8.

Article  CAS  PubMed  Google Scholar 

Developmental engineering: a new paradigm for the design and manufacturing of cell-based products. Part II. From genes to networks: tissue engineering from the viewpoint of systems biology and network science. Tissue Engineering Part B: Reviews 2009;15(4):395–422.

Adhikari S, Nice EC, Deutsch EW, Lane L, Omenn GS, Pennington SR, Paik Y-K, Overall CM, Corrales FJ, Cristea IM et al: A high-stringency blueprint of the human proteome. Nature Communications 2020, 11(1).

Shukla HD, Mahmood J, Vujaskovic Z. Integrated proteo-genomic approach for early diagnosis and prognosis of cancer. Cancer Lett. 2015;369(1):28–36.

Article  CAS  PubMed  Google Scholar 

Mu R, Shen Y, Guo C, Zhang X, Yang H, Yang H. Seven immune-related genes’ prognostic value and correlation with treatment outcome in head and neck squamous cell carcinoma. Mediators Inflamm. 2023;2023:8533476.

Article  PubMed  PubMed Central  Google Scholar 

Paz-González R, Lourido L, Calamia V, Fernández-Puente P, Quaranta P, Picchi F, Blanco FJ, Ruiz-Romero C. An atlas of the knee joint proteins and their role in osteoarthritis defined by literature mining. Mol Cell Proteomics. 2023;22(8):100606.

Article  PubMed  PubMed Central  Google Scholar 

Wan S, Mak M-W, Kung S-Y. mGOASVM: multi-label protein subcellular localization based on gene ontology and support vector machines. BMC Bioinformatics. 2012;13(1):290.

Article  PubMed  PubMed Central  Google Scholar 

The Gene Ontology Consortium. The Gene Ontology Resource: 20 years and still GOing strong. Nucleic Acids Res. 2018;47(D1):D330–8.

Article  PubMed Central  Google Scholar 

Consortium TGO. Gene Ontology Consortium: going forward. Nucleic Acids Res. 2014;43(D1):D1049–56.

Article  Google Scholar 

Ivanov RA, Mukhin AM, Kazantsev FV, Mustafin ZS, Afonnikov DA, Matushkin YG, Lashin SA. Orthoweb: a software package for evolutionary analysis of gene networks. Vavilovskii Zhurnal Genet Selektsii. 2024;28(8):874–81.

CAS  PubMed  PubMed Central  Google Scholar 

Caplan AI. Bone development. Ciba Found Symp. 1988;136:3–21.

CAS  PubMed  Google Scholar 

López JM: Bone development and growth. Int J Mol Sci 2024;25(12).

Sun L, Niu H, Wu Y, Dong S, Li X, Kim BYS, Liu C, Ma Y, Jiang W, Yuan Y. Bio-integrated scaffold facilitates large bone regeneration dominated by endochondral ossification. Bioact Mater. 2024;35:208–27.

CAS  PubMed  PubMed Central  Google Scholar 

Breeland G, Sinkler MA, Menezes RG: Embryology, bone ossification. In: StatPearls. Treasure Island (FL): StatPearls Publishing

Rowe P, Koller A, Sharma S: Physiology, bone remodeling. In: StatPearls. Treasure Island (FL): StatPearls Publishing Copyright © 2025, StatPearls Publishing LLC.; 2025.

Li Z, Yang X, Fu R, Wu Z, Xu S, Jiao J, Qian M, Zhang L, Wu C, Xie T, et al. Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src. Nat Commun. 2024;15(1):1300.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liao R, Dewey MJ, Rong J, Johnson SA, D'Angelo WA, Hussey GS, Badylak SF: Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis. Sci Adv 2024, 10(42):eadn1852.

Fu J, Li S, Ma H, Yang J, Pagnotti GM, Brown LM, Weiss SJ, Mapara MY, Lentzsch S. The checkpoint inhibitor PD-1H/VISTA controls osteoclast-mediated multiple myeloma bone disease. Nat Commun. 2023;14(1):4271.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Graciolli FG, Neves KR, Barreto F, Barreto DV, Dos Reis LM, Canziani ME, Sabbagh Y, Carvalho AB, Jorgetti V, Elias RM, et al. The complexity of chronic kidney disease-mineral and bone disorder across stages of chronic kidney disease. Kidney Int. 2017;91(6):1436–46.

Article  CAS  PubMed  Google Scholar 

Liang W, Feng R, Li X, Duan X, Feng S, Chen J, Li Y, Chen J, Liu Z, Wang X, et al. A RANKL-UCHL1-sCD13 negative feedback loop limits osteoclastogenesis in subchondral bone to prevent osteoarthritis progression. Nat Commun. 2024;15(1):8792.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Matsumoto Y, Larose J, Kent OA, Lim M, Changoor A, Zhang L, Storozhuk Y, Mao X, Grynpas MD, Cong F, et al. RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146. J Clin Invest. 2017;127(4):1303–15.

Article  PubMed  PubMed Central  Google Scholar 

Fogel H, Yeritsyan D, Momenzadeh K, Kheir N, Yeung CM, Abbasian M, Lozano EM, Nazarian RM, Nazarian A. The effect of cannabinoids on single-level lumbar arthrodesis outcomes in a rat model. Spine J. 2024;24(9):1759–72.

Article  PubMed  Google Scholar 

Ikebuchi Y, Aoki S, Honma M, Hayashi M, Sugamori Y, Khan M, Kariya Y, Kato G, Tabata Y, Penninger JM, et al. Coupling of bone resorption and formation by RANKL reverse signalling. Nature. 2018;561(7722):195–200.

Article  CAS  PubMed  Google Scholar 

Altmann B, Rabel K, Kohal RJ, Proksch S, Tomakidi P, Adolfsson E, Bernsmann F, Palmero P, Fürderer T, Steinberg T. Cellular transcriptional response to zirconia-based implant materials. Dent Mater. 2017;33(2):241–55.

Article  CAS  PubMed  Google Scholar 

Wang Z, Sun J, Li Y, Chen C, Xu Y, Zang X, Li L, Meng K. Experimental study of the synergistic effect and network regulation mechanisms of an applied combination of BMP-2, VEGF, and TGF-β1 on osteogenic differentiation. J Cell Biochem. 2020;121(3):2394–405.

Article  CAS  PubMed  Google Scholar 

Ponzetti M, Rucci N. Osteoblast differentiation and signaling: established concepts and emerging topics. Int J Mol Sci. 2021;22(13):6651.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Berendsen AD, Olsen BR. Bone development. Bone. 2015;80:14–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hu L, Chen W, Qian A, Li YP. Wnt/β-catenin signaling components and mechanisms in bone formation, homeostasis, and disease. Bone Res. 2024;12(1):39.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lojk J, Marc J: Roles of non-canonical Wnt signalling pathways in bone biology. Int J Mol Sci 2021;22(19).

Steinhart Z, Angers S. Wnt signaling in development and tissue homeostasis. Development 2018, 145(11).

Yamamichi G, Kato T, Arakawa N, Ino Y, Ujike T, Nakano K, Koh Y, Motoyama Y, Outani H, Myoba S, et al. GDF15 propeptide promotes bone metastasis of castration-resistant prostate cancer by augmenting the bone microenvironment. Biomark Res. 2024;12(1):147.

Article  PubMed  PubMed Central  Google Scholar 

Schmierer B, Hill CS. TGFbeta-SMAD signal transduction: molecular specificity and functional flexibility. Nat Rev Mol Cell Biol. 2007;8(12):970–82.

Article  CAS  PubMed  Google Scholar 

Salazar VS, Gamer LW, Rosen V. BMP signalling in skeletal development, disease and repair. Nat Rev Endocrinol. 2016;12(4):203–21.

Article  CAS  PubMed 

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