Dua HS, Azuara-Blanco A. Limbal stem cells of the corneal epithelium. Surv Ophthalmol. 2000;44:415–25.

Article  CAS  PubMed  Google Scholar 

Tonti E, Manco GA, Spadea L, Zeppieri M. Focus on limbal stem cell deficiency and limbal cell transplantation. World J Transplant. 2023;13:321–30.

Article  PubMed  PubMed Central  Google Scholar 

Bian Y, Jurkunas U. Ocular chemical injuries and limbal stem cell deficiency (LSCD): an update on management. Int Ophthalmol Clin. 2024;64:31–48.

Article  PubMed  Google Scholar 

Aketa N, Kasai M, Noda S, Asano J, Kunieda A, Kawanishi S, et al. Insights into the clinical development of regenerative medical products through a comparison of three cell-based products recently approved for limbal stem cell deficiency. Ocul Surf. 2023;29:220–5.

Article  PubMed  Google Scholar 

Oie Y, Sugita S, Yokokura S, Nakazawa T, Tomida D, Satake Y, et al. Clinical trial of autologous cultivated limbal epithelial cell sheet transplantation for patients with limbal stem cell deficiency. Ophthalmology. 2023;130:608–14.

Article  PubMed  Google Scholar 

Chen W, Nie M, Gan J, Xia N, Wang D, Sun L. Tailoring cell sheets for biomedical applications. Smart Med. 2024;3:e20230038.

Article  PubMed  PubMed Central  Google Scholar 

Langer R, Tirrell DA. Designing materials for biology and medicine. Nature. 2004;428:487–92.

Article  CAS  PubMed  Google Scholar 

Kim DK, Sim BR, Khang G. Nature-Derived Aloe Vera Gel Blended Silk Fibroin Film Scaffolds for Cornea Endothelial Cell Regeneration and Transplantation. ACS Appl Mater Interfaces. 2016;8:15160–8.

Article  CAS  PubMed  Google Scholar 

Salehi AOM, Rafienia M, Mamidi N, Keshel SH, Baradaran-Rafii A. Electrospun aloe vera extract loaded polycaprolactone scaffold for biomedical applications: a promising candidate for corneal stromal regeneration. J Bionic Eng. 2024;21:1949–59.

Article  Google Scholar 

Jameson JF, Pacheco MO, Nguyen HH, Phelps EA, Stoppel WL. Recent advances in natural materials for corneal tissue engineering. Bioengineering. 2021;8:161.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Luo X, He X, Zhao H, Ma J, Tao J, Zhao S, et al. Research progress of polymer biomaterials as scaffolds for corneal endothelium tissue engineering. Nanomaterials. 2023;13:1976.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Orash Mahmoud Salehi A, Heidari-Keshel S, Poursamar SA, Zarrabi A, Sefat F, Mamidi N, et al. Bioprinted membranes for corneal tissue engineering: a review. Pharmaceutics. 2022;14:2797.

Anupama Sekar J, Athira RK, Lakshmi TS, Velayudhan S, Bhatt A, Anil Kumar PR, et al. 3D bioprinting in tissue engineering and regenerative medicine: current landscape and future prospects. In: Bhaskar B, Sreenivasa Rao P, Kasoju N, Nagarjuna V, Baadhe RR, editors. Biomater Tissue Eng Regen Med [Internet]. Singapore: Springer Singapore; 2021 [cited 2021 Jul 23]. p. 561–80. Available from: https://link.springer.com/10.1007/978-981-16-0002-9_17

Jia S, Bu Y, Lau D-SA, Lin Z, Sun T, Lu WW, et al. Advances in 3D bioprinting technology for functional corneal reconstruction and regeneration. Front Bioeng Biotechnol. 2023;10:1065460.

Sponchioni M, Capasso Palmiero U, Moscatelli D. Thermo-responsive polymers: applications of smart materials in drug delivery and tissue engineering. Mater Sci Eng C. 2019;102:589–605.

Article  CAS  Google Scholar 

Anju MS, Raj DK, Madathil BK, Kasoju N, Anil Kumar PR. Intelligent biomaterials for tissue engineering and biomedical applications: current landscape and future prospects. In: Bhaskar B, Sreenivasa Rao P, Kasoju N, Nagarjuna V, Baadhe RR, editors. Biomater Tissue Eng Regen Med [Internet]. Singapore: Springer Singapore; 2021 [cited 2021 Sep 16]. p. 535–60. Available from: https://link.springer.com/10.1007/978-981-16-0002-9_16

Doberenz F, Zeng K, Willems C, Zhang K, Groth T. Thermoresponsive polymers and their biomedical application in tissue engineering – a review. J Mater Chem B. 2020;8:607–28.

Article  CAS  PubMed  Google Scholar 

Nagase K, Yamato M, Kanazawa H, Okano T. Poly( N -isopropylacrylamide)-based thermoresponsive surfaces provide new types of biomedical applications. Biomaterials. 2018;153:27–48.

Article  CAS  PubMed  Google Scholar 

Lanzalaco S, Armelin E. Poly(N-isopropylacrylamide) and copolymers: a review on recent progresses in biomedical applications. Gels. 2017;3:36.

Article  PubMed  PubMed Central  Google Scholar 

Shaibie NA, Ramli NA, Mohammad Faizal NDF, Srichana T, Mohd Amin MCI. Poly(N-isopropylacrylamide)-based polymers: recent overview for the development of temperature-responsive drug delivery and biomedical applications. Macromol Chem Phys. 2023;224:2300157.

Article  CAS  Google Scholar 

Joseph N, Prasad T, Raj V, Anil Kumar PR, Sreenivasan K, Kumary TV. A Cytocompatible poly(N-isopropylacrylamide-co-glycidylmethacrylate) coated surface as new substrate for corneal tissue engineering. J Bioact Compat Polym. 2010;25:58–74.

Article  CAS  Google Scholar 

Nithya J, Kumar PRA, Tilak P, Leena J, Sreenivasan K, Kumary TV. Intelligent thermoresponsive substrate from modified overhead projection sheet as a tool for construction and support of cell sheets in vitro. Tissue Eng Part C Methods. 2011;17:181–91.

Article  CAS  PubMed  Google Scholar 

Madathil BK, Kumar PRA, Kumary TV. N-isopropylacrylamide-co-glycidylmethacrylate as a thermoresponsive substrate for corneal endothelial cell sheet engineering. BioMed Res Int. 2014;2014:450672.

Article  PubMed  PubMed Central  Google Scholar 

Venugopal B, Shenoy SJ, Mohan S, Anil Kumar PR, Kumary TV. Bioengineered corneal epithelial cell sheet from mesenchymal stem cells—a functional alternative to limbal stem cells for ocular surface reconstruction. J Biomed Mater Res B Appl Biomater. 2020;108:1033–45.

Article  CAS  PubMed  Google Scholar 

Venugopal B, Mohan S, Kumary TV, Anil Kumar PR. peripheral blood as a source of stem cells for regenerative medicine: emphasis towards corneal epithelial reconstruction—an in vitro study. Tissue Eng Regen Med. 2020;17:495–510.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Praveen W, Madathil BK, Sajin Raj RS, Kumary TV, Anil Kumar PR. A flexible thermoresponsive cell culture substrate for direct transfer of keratinocyte cell sheets. Biomed Mater. 2017;12:065012.

Article  PubMed  Google Scholar 

Madathil BK, Sundaran PS, Kumary TV, Bhatt A, Anil Kumar PR. Biofunctionalised polycaprolactone fibrous mat as a transfer tool for cell sheet engineering. Fibers Polym. 2017;18:2094–101.

Article  CAS  Google Scholar 

Joe AW, Yeung SN. Concise review: identifying limbal stem cells: classical concepts and new challenges. Stem Cells Transl Med. 2014;3:318–22.

Article  CAS  PubMed  Google Scholar 

Romo-Valera C, Pérez-Garrastachu M, Hernáez-Moya R, Rodriguez-Astigarraga M, Romano-Ruiz P, Etxebarria J, et al. Characterisation of corneas following different time and storage methods for their use as a source of stem-like limbal epithelial cells. Exp Eye Res. 2021;211:108720.

Article  CAS  PubMed  Google Scholar 

Shaharuddin B, Ahmad S, Md Latar N, Ali S, Meeson A. A human corneal epithelial cell line model for limbal stem cell biology and limbal immunobiology. Stem Cells Transl Med. 2017;6:761–6.

Article  CAS  PubMed  Google Scholar 

Kasza G, Stumphauser T, Bisztrán M, Szarka G, Hegedüs I, Nagy E, et al. Thermoresponsive poly(N, N-diethylacrylamide-co-glycidyl methacrylate) copolymers and its catalytically active α-chymotrypsin bioconjugate with enhanced enzyme stability. Polymers. 2021;13:987.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fan X, Gu S, Wu L, Yang L. Preparation and characterization of thermoresponsive poly( N -isopropylacrylamide) copolymers with enhanced hydrophilicity. E-Polym. 2020;20:561–70.

Article  CAS  Google Scholar 

Ilić-Stojanović S, Urošević M, Nikolić L, Petrović D, Stanojević J, Najman S, et al. Intelligent poly(N-isopropylmethacrylamide) hydrogels: synthesis, structure characterization, stimuli-responsive swelling properties, and their radiation decomposition. Polymers. 2020;12:1112.

Article  PubMed  PubMed Central  Google Scholar 

Pham Q-T, Yao Z-H, Chang Y-T, Wang F-M, Chern C-S. LCST phase transition kinetics of aqueous poly(N-isopropylacrylamide) solution. J Taiwan Inst Chem Eng. 2018;93:63–9.

Article  CAS  Google Scholar 

Sousa RG, Magalhães WF, Freitas RFS. Glass transition and thermal stability of poly(N-isopropylacrylamide) gels and some of their copolymers with acrylamide. Polym Degrad Stab. 1998;61:275–81.