Allen, N. J. & Lyons, D. A. Glia as architects of central nervous system formation and function. Science 362, 181–185 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Khakh, B. S. & Sofroniew, M. V. Diversity of astrocyte functions and phenotypes in neural circuits. Nat. Neurosci. 18, 942–952 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nagai, J. et al. Behaviorally consequential astrocytic regulation of neural circuits. Neuron 109, 576–596 (2021).

Article  CAS  PubMed  Google Scholar 

Khakh, B. S. & Deneen, B. The emerging nature of astrocyte diversity. Annu. Rev. Neurosci. 42, 187–207 (2019).

Article  CAS  PubMed  Google Scholar 

Ben Haim, L. & Rowitch, D. H. Functional diversity of astrocytes in neural circuit regulation. Nat. Rev. Neurosci. 18, 31–41 (2017).

Article  CAS  PubMed  Google Scholar 

Soto, J. S. et al. Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms. Nature 616, 764–773 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Uezu, A. et al. Identification of an elaborate complex mediating postsynaptic inhibition. Science 353, 1123–1129 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Heffernan, K. S., Rahman, K., Smith, Y. & Galvan, A. Characterization of the GfaABC1D promoter to selectively target astrocytes in the rhesus macaque brain. J. Neurosci. Methods 372, 109530 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cahoy, J. D. et al. A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J. Neurosci. 28, 264–278 (2008).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chai, H. et al. Neural circuit-specialized astrocytes: transcriptomic, proteomic, morphological, and functional evidence. Neuron 95, 531–549 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Burda, J. E. & Sofroniew, M. V. Reactive gliosis and the multicellular response to CNS damage and disease. Neuron 81, 229–248 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Linnerbauer, M., Wheeler, M. A. & Quintana, F. J. Astrocyte crosstalk in CNS inflammation. Neuron 108, 608–622 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Roux, K. J., Kim, D. I., Raida, M. & Burke, B. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J. Cell Biol. 196, 801–810 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Castle, M. J., Turunen, H. T., Vandenberghe, L. H. & Wolfe, J. H. Controlling AAV tropism in the nervous system with natural and engineered capsids. Methods Mol. Biol. 1382, 133–149 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Diaz-Castro, B., Gangwani, M. R., Yu, X., Coppola, G. & Khakh, B. S. Astrocyte molecular signatures in Huntington’s disease. Sci. Transl. Med. 11, eaaw8546 (2019).

Article  CAS  PubMed  Google Scholar 

Yu, X. et al. Context-specific striatal astrocyte molecular responses are phenotypically exploitable. Neuron 108, 1146–1162.e1110 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yu, X. et al. Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior. Neuron 99, 1170–1187 e1179 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cetin, A., Komai, S., Eliava, M., Seeburg, P. H. & Osten, P. Stereotaxic gene delivery in the rodent brain. Nat. Protoc. 1, 3166–3173 (2006).

Article  CAS  PubMed  Google Scholar 

Lee, Y., Messing, A., Su, M. & Brenner, M. GFAP promoter elements required for region-specific and astrocyte-specific expression. Glia 56, 481–493 (2008).

Article  PubMed  Google Scholar 

Shigetomi, E. et al. Imaging calcium microdomains within entire astrocyte territories and endfeet with GCaMPs expressed using adeno-associated viruses. J. Gen. Physiol. 141, 633–647 (2013).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Koerber, J. T. et al. Molecular evolution of adeno-associated virus for enhanced glial gene delivery. Mol. Ther. 17, 2088–2095 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Feist, P. & Hummon, A. B. Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples. Int. J. Mol. Sci. 16, 3537–3563 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pajarillo, E., Rizor, A., Lee, J., Aschner, M. & Lee, E. The role of astrocytic glutamate transporters GLT-1 and GLAST in neurological disorders: potential targets for neurotherapeutics. Neuropharmacology 161, 107559 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tong, X. et al. Astrocyte Kir4.1 ion channel deficits contribute to neuronal dysfunction in Huntington’s disease model mice. Nat. Neurosci. 17, 694–703 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang, X., Wan, J. Q. & Tong, X. P. Potassium channel dysfunction in neurons and astrocytes in Huntington’s disease. CNS Neurosci. Ther. 24, 311–318 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Cho, K. F. et al. Proximity labeling in mammalian cells with TurboID and split-TurboID. Nat. Protoc. 15, 3971–3999 (2020).

Article  CAS  PubMed  Google Scholar 

Rayaprolu, S. et al. Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain. Nat. Commun. 13, 2927 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lackner, D. H., Schmidt, M. W., Wu, S., Wolf, D. A. & Bähler, J. Regulation of transcriptome, translation, and proteome in response to environmental stress in fission yeast. Genome Biol. 13, R25 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Laurent, J. M. et al. Protein abundances are more conserved than mRNA abundances across diverse taxa. Proteomics 10, 4209–4212 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu, Y., Beyer, A. & Aebersold, R. On the dependency of cellular protein levels on mRNA abundance. Cell 165, 535–550 (2016).

Article  CAS  PubMed  Google Scholar 

Dowell, J. A., Johnson, J. A. & Li, L. Identification of astrocyte secreted proteins with a combination of shotgun proteomics and bioinformatics. J. Proteome Res. 8, 4135–4143 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Frese, C. K. et al. Quantitative map of proteome dynamics during neuronal differentiation. Cell Rep. 18, 1527–1542 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Basu, S., Campbell, H. M., Dittel, B. N. & Ray, A. Purification of specific cell population by fluorescence activated cell sorting (FACS). J. Vis. Exp. https://doi.org/10.3791/1546 (2010).

Article  PubMed  PubMed Central  Google Scholar 

Foo, L. C. Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting. Cold Spring Harb. Protoc. 2013, 551–560 (2013).

Article  PubMed  Google Scholar 

Pan, J. & Wan, J. Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain. J. Immunol. Methods 486, 112834 (2020).

Article  CAS  PubMed