To control insects, weeds, and infections in crops, old-fashioned pesticide formulations (with massive quantities of heavy metals and a variety of chemicals) are used. By biological amplification via the food chain, many of these established pesticide formulations have accumulated in living systems and caused environmental pollution. To form a nanoparticulate matrix with a diameter ranging from 322.2 ± 0.9 to 403.7 ± 0.7 nm, mancozeb was embedded in chitosan–gum acacia (CSGA) biopolymers and loadings were confirmed via TEM and FTIR. Differential scanning calorimetry analyses were carried out as part of the investigation. Inhibition of Alternaria alternata by nanoparticles (NPs) with 1.0 mg/mL mancozeb (CSGA-1.0) was 85.2 ± 0.7 % at 0.5 ppm, whereas for Stemphylium lycopersici it was 62.1 ± 0.7% in the mycelium inhibition method. NPs demonstrated antimicrobial action in pot house environments. After ten hours, the mancozeb was liberated from the nanoformulations due to polymer matrix diffusion and relaxation, compared to 2 h for commercial mancozeb. Even while drug-loaded conjugated nanoparticles have equivalent antifungal activities, they have a lower release rate and, hence, reduced toxicology compared to commercial mancozeb. Therefore, this method can be employed to implement sustainable farming techniques in the future. View Full-Text ►▼ Show Figures This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.