Evaluation and comparison of the anti-proliferative and anti-metastatic effects of urolithin A and urolithin B against esophageal cancer cells: an in vitro and in silico study

An J et al. Natural products for esophageal Cancer therapy: from traditional medicine to modern drug discovery. Int J Mol Sci. 2022;23(21): 13558

Sung H, et al. Global Cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71(3):209– 249.

PubMed  Google Scholar 

Ilson DH. Esophageal cancer chemotherapy: recent advances. Gastrointest Cancer Res. 2008;2(2):85–92.

PubMed  PubMed Central  Google Scholar 

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016;66(1):7–30.

PubMed  Google Scholar 

Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495–516.

CAS  PubMed  PubMed Central  Google Scholar 

Liberal J, et al. Urolithins impair cell proliferation, arrest the cell cycle and induce apoptosis in UMUC3 bladder cancer cells. Invest New Drugs. 2017;35(6):671–81.

CAS  PubMed  Google Scholar 

Cerdá B, Tomás-Barberán FA, Espín JC. Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans: identification of biomarkers and individual variability. J Agric Food Chem. 2005;53(2):227–35.

PubMed  Google Scholar 

García-Villalba R, et al. Time course production of urolithins from ellagic acid by human gut microbiota. J Agric Food Chem. 2013;61(37):8797–806.

PubMed  Google Scholar 

Nuñez-Sánchez MA, et al. Targeted metabolic profiling of pomegranate polyphenols and urolithins in plasma, urine and colon tissues from colorectal cancer patients. Mol Nutr Food Res. 2014;58(6):1199–211.

PubMed  Google Scholar 

Al-Harbi SA, et al. Urolithins: the gut based polyphenol metabolites of ellagitannins in Cancer prevention, a review. Front Nutr. 2021;8:647582.

PubMed  PubMed Central  Google Scholar 

Mirzaei S, et al. Urolithins increased anticancer effects of chemical drugs, ionizing radiation and hyperthermia on human esophageal carcinoma cells in vitro. Tissue Cell. 2022;77:101846.

CAS  PubMed  Google Scholar 

Riss TL, Niles MR, Cell Viability Assays. AL. 2013 May 1 [Updated 2016 Jul 1]. In: Markossian S, Grossman A, Baskir H, editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences. 2004

Poonaki E, et al. Targeting BMI-1 with PLGA–PEG nanoparticle-containing PTC209 modulates the behavior of human glioblastoma stem cells and cancer cells. Cancer Nanotechnol. 2021;12(1):5.

CAS  Google Scholar 

Jalili-Nik M, et al. Zerumbone promotes cytotoxicity in human malignant glioblastoma cells through reactive oxygen species (ROS) generation. Oxid Med Cell Longev. 2020;2020:3237983.

PubMed  PubMed Central  Google Scholar 

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2– ∆∆CT method. Methods. 200;25(4): 402–8.

Jalili-Nik M, et al. Zerumbone, a ginger sesquiterpene, inhibits migration, invasion, and metastatic behavior of human malignant glioblastoma multiforme in vitro. BioFactors. 2021;47(5):729–39.

CAS  PubMed  Google Scholar 

Samantaray S, et al. Increased expression of MMP-2 and MMP-9 in esophageal squamous cell carcinoma. J Cancer Res Clin Oncol. 2004;130(1):37–44.

CAS  PubMed  Google Scholar 

An J, et al. Natural products for esophageal Cancer therapy: from traditional medicine to modern drug discovery. Int J Mol Sci. 2022;23(21):13558.

CAS  PubMed  PubMed Central  Google Scholar 

Tomás-Barberán FA et al. Urolithins, the rescue of old metabolites to understand a new concept: metabotypes as a nexus among phenolic metabolism, microbiota dysbiosis, and host health status. Mol Nutr Food Res. 2017;61(1).

Totiger TM, et al. Urolithin A, a novel natural compound to target PI3K/AKT/mTOR pathway in pancreatic Cancer. Mol Cancer Ther. 2019;18(2):301–11.

CAS  PubMed  Google Scholar 

Zhao W, et al. Metabolite of ellagitannins, urolithin A induces autophagy and inhibits metastasis in human sw620 colorectal cancer cells. Mol Carcinog. 2018;57(2):193–200.

CAS  PubMed  Google Scholar 

Lv My, et al. Urolithin B suppresses tumor growth in hepatocellular carcinoma through inducing the inactivation of Wnt/β-catenin signaling. J Cell Biochem. 2019;120(10):17273–82.

CAS  PubMed  Google Scholar 

Alzahrani AM et al. Urolithin A and B alter cellular metabolism and induce metabolites associated with apoptosis in leukemic cells. Int J Mol Sci. 2021;22(11):5465

González-Sarrías A, et al. Antiproliferative activity of the ellagic acid-derived gut microbiota Isourolithin A and comparison with its urolithin A isomer: the role of cell metabolism. Eur J Nutr. 2017;56(2):831–41.

PubMed  Google Scholar 

Bobowska A, et al. Comparative studies of urolithins and their phase II metabolites on macrophage and neutrophil functions. Eur J Nutr. 2021;60(4):1957–72.

CAS  PubMed  Google Scholar 

Daina A, Michielin O, Zoete V. SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small mTekin HO et al. Molecular Polar surface area, total solventolecules. Sci Rep. 2017;7(1):42717.

PubMed  PubMed Central  Google Scholar 

Tekin HO et al. Molecular Polar surface area, total solvent accessible surface area (SASA), heat of formation, and Gamma-Ray Attenuation properties of some flavonoids. Front Phys. 2022;10:838725

Prasanna S, Doerksen RJ. Topological Polar surface area: a useful descriptor in 2D-QSAR. Curr Med Chem. 2009;16(1):21–41.

CAS  PubMed  PubMed Central  Google Scholar 

Giménez-Bastida JA, et al. The gut microbiota metabolite urolithin A, but not other relevant urolithins, induces p53-dependent cellular senescence in human colon cancer cells. Food Chem Toxicol. 2020;139:111260.

PubMed  Google Scholar 

Tajvar Nasab N, et al. Urolithin B inhibits proliferation and migration and promotes apoptosis and necrosis by inducing G2/M arrest and targeting MMP-2/-9 expression in osteosarcoma cells. J Biochem Mol Toxicol. 2023;37(12):e23486.

CAS  PubMed  Google Scholar 

Eidizade F, et al. Inhibition of glioblastoma proliferation, invasion, and migration by urolithin B through inducing G0/G1 arrest and targeting MMP-2/-9 expression and activity. BioFactors. 2023;49(2):379–89.

CAS  PubMed  Google Scholar 

Heilman J, et al. Safety assessment of urolithin A, a metabolite produced by the human gut microbiota upon dietary intake of plant derived ellagitannins and ellagic acid. Food Chem Toxicol. 2017;108:289–97.

CAS  PubMed  Google Scholar 

El-Wetidy MS, et al. Urolithin A induces cell cycle arrest and apoptosis by inhibiting Bcl-2, increasing p53-p21 proteins and reactive oxygen species production in colorectal cancer cells. Cell Stress Chaperones. 2021;26(3):473–93.

CAS  PubMed  PubMed Central  Google Scholar 

Redza-Dutordoir M. D.A. Averill-Bates 2016 Activation of apoptosis signalling pathways by reactive oxygen species. Biochim Et Biophys Acta (BBA) - Mol Cell Res 1863 12 2977–92.

CAS  Google Scholar 

Halliwell B. Free radicals and antioxidants - quo vadis? Trends Pharmacol Sci. 2011;32(3):125–30.

CAS  PubMed  Google Scholar 

Chen J-S, et al. Sonic Hedgehog signaling pathway induces cell migration and invasion through focal adhesion kinase/akt signaling-mediated activation of matrix metalloproteinase (MMP)-2 and MMP-9 in liver cancer. Carcinogenesis. 2013;34(1):10–9.

CAS  PubMed  Google Scholar 

Kim E-S, Kim M-S, Moon A. TGF-β-induced upregulation of MMP-2 and MMP-9 depends on p38 MAPK, but not ERK signaling in MCF10A human breast epithelial cells. Int J Oncol. 2004;25(5):1375–82.

CAS  PubMed  Google Scholar 

Adya R, et al. Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis. Cardiovascular Res. 2008;78(2):356–65.

CAS  Google Scholar 

Yeh CB, et al. Antimetastatic effects of Norcantharidin on hepatocellular carcinoma by transcriptional Inhibition of MMP-9 through modulation of NF-kB activity. PLoS ONE. 2012;7(2):e31055.

CAS  PubMed  PubMed Central  Google Scholar 

Besli N, et al. Metformin suppresses the proliferation and invasion through NF-kB and MMPs in MCF-7 cell line. Turkish J Biochem. 2020;45(3):295–304.

CAS  Google Scholar 

Comments (0)

No login
gif