Lucien Reclaru, Norina Consuela Forna, Doriana Agop Forna, Mihaela Pantea

DOI: 10.62610/RJOR.2025.2.17.1

ABSTRACT

Aim of the study: Silver contained in dental alloys is not mutagenic, carcinogenic or toxic to reproduction. However, the European Chemicals Agency (ECHA) has decided to include ionic silver and silver nano-particles in the list of biocidal substances. In this context, we aimed to evaluate the quantities of silver released and the cytotoxicity of various dental alloys containing silver, sourced from two distinct suppliers present in the EU (European Union) markets. Materials and methods: Five dental alloys (coded from #1 to #5), with silver contents ranging from 9.2% to 58%, were tested by extraction tests in a solution of racemic lactic acid and sodium chloride (7-day extraction at 37°C). The cytotoxic behaviour of five other dental alloys (coded from #6 to #10), with silver contents ranging from 22.4% to 59%, was also evaluated. Results: Extraction test results indicate small quantities of silver, ranging from 55μg/L to 243μg/L. Cytotoxicity assessment indicated cytotoxic levels of 0 and 1, except for alloy #9, which showed moderate cytotoxicity will be structured as shown here. Conclusions: The quantities of silver detected during the extraction tests were minimal, however, the assessment of alloys’ cytotoxicity reveals variability of the obtained results.