Recent Advances in Three-Dimensional Models for Studies of Liver Fibrosis

Trefts E, Gannon M, Wasserman DH. The liver. Curr Biol. 2017;27:R1147–51.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sharma A, Nagalli S. Chronic liver disease. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024.

Younossi ZM, Golabi P, Paik JM, Henry A, Van Dongen C, Henry L. The global epidemiology of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH): a systematic review. Hepatology. 2023;77:1335–47.

Article  PubMed  Google Scholar 

Cheemerla S, Balakrishnan M. Global epidemiology of chronic liver disease. Clin Liver Dis (Hoboken). 2021;17:365–70.

Article  PubMed  Google Scholar 

Jung YK, Yim HJ. Reversal of liver cirrhosis: current evidence and expectations. Korean J Intern Med. 2017;32:213–28.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bayarsaikhan D, Bayarsaikhan G, Kang HA, Lee SB, Han SH, Okano T, et al. A study on iPSC-associated factors in the generation of hepatocytes. Tissue Eng Regen Med. 2024;21:1245–54.

Article  CAS  PubMed  Google Scholar 

Kim JH, Jang YJ, An SY, Son J, Lee J, Lee G, et al. Enhanced metabolizing activity of human ES cell-derived hepatocytes using a 3D culture system with repeated exposures to xenobiotics. Toxicol Sci. 2015;147:190–206.

Article  CAS  PubMed  Google Scholar 

Park JY, Han J, Jung HS, Lee G, Kim HJ, Cho GS, et al. Synthetic probes for in vitro purification and in vivo tracking of hepatocytes derived from human pluripotent stem cells. Biomaterials. 2019;222: 119431.

Article  CAS  PubMed  Google Scholar 

Duval K, Grover H, Han LH, Mou Y, Pegoraro AF, Fredberg J, et al. Modeling physiological events in 2D vs. 3D cell culture. Physiology (Bethesda). 2017;32:266–77.

CAS  PubMed  Google Scholar 

Lancaster MA, Knoblich JA. Organogenesis in a dish: modeling development and disease using organoid technologies. Science. 2014;345:1247125.

Article  PubMed  Google Scholar 

Bataller R, Brenner DA. Liver fibrosis. J Clin Invest. 2005;115:209–18.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Friedman SL. Liver fibrosis – from bench to bedside. J Hepatol. 2003;38:S38-53.

Article  PubMed  Google Scholar 

Kisseleva T, Brenner D. Molecular and cellular mechanisms of liver fibrosis and its regression. Nat Rev Gastroenterol Hepatol. 2021;18:151–66.

Article  PubMed  Google Scholar 

Tsuchida T, Friedman SL. Mechanisms of hepatic stellate cell activation. Nat Rev Gastroenterol Hepatol. 2017;14:397–411.

Article  CAS  PubMed  Google Scholar 

Friedman SL. Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver. Physiol Rev. 2008;88:125–72.

Article  CAS  PubMed  Google Scholar 

Chen K, Xu M, Lu F, He Y. Development of matrix metalloproteinases-mediated extracellular matrix remodeling in regenerative medicine: a mini review. Tissue Eng Regen Med. 2023;20:661–70.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen L, Brenner DA, Kisseleva T. Combatting fibrosis: exosome-based therapies in the regression of liver fibrosis. Hepatol Commun. 2018;3:180–92.

Article  PubMed  PubMed Central  Google Scholar 

Xu AL, Han L, Yan J, Liu D, Wang W. Effects of mesenchymal stem cells-derived extracellular vesicles on inhibition of hepatic fibrosis by delivering miR-200a. Tissue Eng Regen Med. 2024;21:609–24.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Friedman SL, Rockey DC, McGuire RF, Maher JJ, Boyles JK, Yamasaki G. Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture. Hepatology. 1992;15:234–43.

Article  CAS  PubMed  Google Scholar 

Lee G, Kim H, Park JY, Kim G, Han J, Chung S, et al. Generation of uniform liver spheroids from human pluripotent stem cells for imaging-based drug toxicity analysis. Biomaterials. 2021;269:120529.

Article  CAS  PubMed  Google Scholar 

Lee DH, Yun DW, Kim YH, Im GB, Hyun J, Park HS, et al. Various three-dimensional culture methods and cell types for exosome production. Tissue Eng Regen Med. 2023;20:621–35.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brovold M, Keller D, Soker S. Differential fibrotic phenotypes of hepatic stellate cells within 3D liver organoids. Biotechnol Bioeng. 2020;117:2516–26.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mannaerts I, Thoen LFR, Eysackers N, Cubero FJ, Leite SB, Coldham I, et al. Unfolded protein response is an early, non-critical event during hepatic stellate cell activation. Cell Death Dis. 2019;10:98.

Article  PubMed  PubMed Central  Google Scholar 

Sorrentino G, Rezakhani S, Yildiz E, Nuciforo S, Heim MH, Lutolf MP, et al. Mechano-modulatory synthetic niches for liver organoid derivation. Nat Commun. 2020;11:3416.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Septiana WL, Pawitan JA. Potential use of organoids in regenerative medicine. Tissue Eng Regen Med. 2024;21:1125–39.

Article  PubMed  Google Scholar 

Kammerer S. Three-dimensional liver culture systems to maintain primary hepatic properties for toxicological analysis in vitro. Int J Mol Sci. 2021;22:10214.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee SY, Koo IS, Hwang HJ, Lee DW. In vitro three-dimensional (3D) cell culture tools for spheroid and organoid models. SLAS Discov. 2023;28:119–37.

Article  CAS  PubMed  Google Scholar 

Urzi O, Gasparro R, Costanzo E, De Luca A, Giavaresi G, Fontana S, et al. Three-dimensional cell cultures: the bridge between in vitro and in vivo models. Int J Mol Sci. 2023;24:12046.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Juarez-Moreno K, Chavez-Garcia D, Hirata G, Vazquez-Duhalt R. Monolayer (2D) or spheroids (3D) cell cultures for nanotoxicological studies? Comparison of cytotoxicity and cell internalization of nanoparticles. Toxicol In Vitro. 2022;85:105461.

Article  CAS  PubMed  Google Scholar 

Zhu T, Hu Y, Cui H, Cui H. 3D multispheroid assembly strategies towards tissue engineering and disease modeling. Adv Healthc Mater. 2024;13:e2400957.

Article  PubMed  Google Scholar 

Zhang CY, Yuan WG, He P, Lei JH, Wang CX. Liver fibrosis and hepatic stellate cells: etiology, pathological hallmarks and therapeutic targets. World J Gastroenterol. 2016;22:10512–22.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Leite SB, Roosens T, El Taghdouini A, Mannaerts I, Smout AJ, Najimi M, et al. Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro. Biomaterials. 2016;78:1–10.

Article  CAS  PubMed 

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