A diluted one-step warming protocol: survival of vitrified blastocyst

Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries. Reprod Biomed Online. 2007;14:72–9.

Article  PubMed  Google Scholar 

Bottrel M, Hidalgo M, Mogas T, Pereira B, Ortiz I, Díaz-Jiménez M, Consuegra C, Morató R, Dorado J. One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos. Theriogenology. 2020;152:47–52.

Article  CAS  PubMed  Google Scholar 

Caamaño JN, Gómez E, Trigal B, Muñoz M, Carrocera S, Martín D, Díez C. Survival of vitrified invitro-produced bovine embryos after a one-step warming in-straw cryoprotectant dilution procedure. Theriogenology. 2015;83:881–90.

Article  PubMed  Google Scholar 

Canesin HS, Ortiz I, Rocha Filho AN, Salgado RM, Brom-de-Luna JG, Hinrichs K. Effect of warming method on embryo quality in a simplified equine embryo vitrification system. Theriogenology. 2020;151:151–8.

Article  CAS  PubMed  Google Scholar 

Casciani LRV, Monseur B, Cimadomo D, Alvero R. Oocyte and embryo cryopreservation in assisted reproductive technology: past achievements and current challenges. Fertil Steril. 2023;120:506–20.

Article  PubMed  Google Scholar 

Chang CC, Shapiro DB, Nagy ZP. The effects of vitrification on oocyte quality. Biol Reprod. 2022;106:316–27.

Article  PubMed  Google Scholar 

El-Danasouri I, Selman H. Successful pregnancies and deliveries after a simple vitrification protocol for day 3 human embryos. Fertil Steril. 2001;76:400–2.

Article  CAS  PubMed  Google Scholar 

Gallardo M, Saenz J, Risco R. Human oocytes and zygotes are ready for ultra-fast vitrification after 2 minutes of exposure to standard CPA solutions. Sci Rep. 2019;9:1–9.

Article  CAS  Google Scholar 

Gosden RG. General principles of cryopreservation. Methods Mol Biol. 2014;1154:261–8.

Article  PubMed  Google Scholar 

Isachenko V, Selman H, Isachenko E, Montag M, El-Danasoun I, Nawroth F. Modified vitrification of human pronuclear oocytes: Efficacy and effect on ultrastructure. Reprod Biomed Online. 2003;7:211–6.

Article  PubMed  Google Scholar 

Jia Y, Yang X, Zhang C, Yang S, Li M, Xu W, Ji Q, Jiang H. Cryosurvival and pregnancy rates: one-step protocol for freezing–thawing Shangri-la Yak (Bos grunniens) Embryos. Reprod Domest Anim. 2018;53:1168–75.

Article  CAS  PubMed  Google Scholar 

Kaskar KS, Sieren K, Brabner V, Huneke B, VerMilyea L, VerMilyea T. Effect of reducing the vitrification and warming times on blastocyst survival and pregnancy rates. Fertil Steril. 2023;120:e142–3.

Article  Google Scholar 

Kupka MS, D’hooghe T, Ferraretti AP, de Mouzon J, Erb K, CastillaAlcalá JA, Calhaz-Jorge C, De Geyter C, Goossens V, Strohmer H, Petrovskaya E, Tishkevich O, Wyns C, Kyurkchiev S, Antonova I, Pelekanos M, Rezabek K, Markova MJ, Lemmen J, Sõritsa D, Gissler M, Tiitinen A, Royere D, Tandler-Schneider A, Loutradis D, Tarlatzis BC, Kosztolanyi G, Urbancsek J, Bjorgvinsson H, Mocanu E, Scaravelli G, Lokshin V, Ravil V, Gudleviciene Z, Belo Lopes G, Moshin V, Simic TM, Vukicevic D, Romundstad LB, Kurzawa R, Laranjeira AR, Rugescu I, Doroftei B, Korsak V, Radunovic N, Tabs N, Tomazevic T, Virant-Klun I, Hernandez Hernandez J, Bergh C, Weder M, Smeenk JMJ, Gryshchenko M, Baranowski R, Assisted reproductive technology in Europe. Results generated from European registers by ESHRE. Hum Reprod. 2011;31(2016):233–48.

Google Scholar 

Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online. 2005;11:300–8.

Article  PubMed  Google Scholar 

Liebermann J, Hrvojevic K, Hirshfeld-Cytron J, Brohammer R, Wagner Y, Susralski A, Jasulaitis S, Chan S, Takhsh E, Uhler M. Fast and furious: pregnancy outcome with one-step rehydration in the warming protocol for human blastocysts. Reprod Biomed Online. 2024;48:1–8.

Article  Google Scholar 

Manns JN, Katz S, Whelan J, Patrick JL, Holt T, Merline AM, Taylor TH. Validation of a new, ultra-fast blastocyst warming technique reduces warming times to 1 min and yields similar survival and re-expansion compared to blastocysts warmed using a standard method. Fertil Steril. 2021;116:e165.

Article  Google Scholar 

Masrat-Un-Nisa AA, Malik KA, Sofi A, Khatun N. Yousuf, Recent advancements in vitrification cryodevices for gamete and gonadal tissue. Cryo-Letters. 2022;43:129–39.

Article  PubMed  Google Scholar 

Morató R, Mogas T. New device for the vitrification and in-straw warming of in vitro produced bovine embryos. Cryobiology. 2014;68:288–93.

Article  PubMed  Google Scholar 

De Munck N, Vajta G. Safety and efficiency of oocyte vitrification. Cryobiology. 2017;78:119–27.

Article  PubMed  Google Scholar 

Rienzi L, Gracia C, Maggiulli R, Labarbera AR, Kaser DJ, Ubaldi FM, Vanderpoel S, Racowsky C. Oocyte, embryo and blastocyst cryopreservation in art: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance. Hum Reprod Update. 2017;23:139–55.

CAS  PubMed  Google Scholar 

Seki S, Mazur P. The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology. 2009;59:75–82.

Article  PubMed  PubMed Central  Google Scholar 

Serdarogullari M, Coban O, Boynukalin FK, Bilgin EM, Findikli N, Bahceci M. Successful application of a single warming protocol for embryos cryopreserved by either slow freezing or vitrification techniques. Syst Biol Reprod Med. 2019;65:12–9.

Article  CAS  PubMed  Google Scholar 

Simopoulou M, Asimakopoulos B, Bakas P, Boyadjiev N, Tzanakaki D, Creatsas G. Oocyte and embryo vitrification in the IVF laboratory: a comprehensive review. Folia Med (Plovdiv). 2014;56:161–9.

Article  PubMed  Google Scholar 

Taylor TH, Manns JN, Katz I, Patrick JL, Holt T, Katz SE. Ultrafast warming protocol demonstrates similar outcomes and significantly decreases embryology workload compared to standard warming protocols, a randomized control trial with euploid blastocysts. Fertil Steril. 2022;118:e150.

Article  Google Scholar 

Vajta G, Nagy ZP. Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod Biomed Online. 2006;12:779–96.

Article  PubMed  Google Scholar 

Xu H, Wang X, Tao R, Bi J, He X, Zhu F, Liu K, Xu Y, Li J. Optimal stage for cryotop vitrification of porcine embryos. Cell Reprogram. 2022;24:132–41.

Article  CAS  PubMed  Google Scholar 

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