Low Molecular Weight Peptides Derived from Iranian Scorpion (Odontobuthus bidentatus) Venom Induces Apoptosis in the Hepatocellular Carcinoma Cell Line (HepG2) in 3D Cell Culture

Ali SA, Alam M, Abbasi A, Undheim EA, Fry BG, Kalbacher H et al (2016) Structure-activity relationship of chlorotoxin-like peptides. Toxins 8(2):36

Article  PubMed  PubMed Central  Google Scholar 

Alikhani HK, Bidmeshkipour A, Zargan J (2020) Cytotoxic and apoptotic induction effects of the venom of Iranian scorpion (Odontobuthus Bidentatus) in the Hepatocellular carcinoma cell line (HepG2). Int J Pept Res Ther. 26:1–10

Andersen T, Auk-Emblem P, Dornish M (2015) 3D cell culture in alginate hydrogels. Microarrays 4(2):133–161

Article  CAS  PubMed  PubMed Central  Google Scholar 

Azimian H, Dayyani M, Toossi MTB, Mahmoudi M (2018) Bax/Bcl-2 expression ratio in prediction of response to breast cancer radiotherapy. Iran J Basic Med Sci 21(3):325

PubMed  PubMed Central  Google Scholar 

Chai J, Yang W, Gao Y, Guo R, Peng Q, Abdel-Rahman MA et al (2021) Antitumor effects of scorpion peptide Smp43 through mitochondrial dysfunction and membrane disruption on hepatocellular carcinoma. J Nat Prod 84(12):3147–3160

Article  CAS  PubMed  Google Scholar 

Collins AR (2004) The comet assay for DNA damage and repair. Mol Biotechnol 26(3):249

Article  CAS  PubMed  Google Scholar 

D’Suze G, Rosales A, Salazar V, Sevcik CJT (2010) Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line. 56(8):1497–1505

Deshane J, Garner CC (2003) Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2. 278(6):4135–4144Sontheimer HJJobc

Díaz-García A, Ruiz-Fuentes JL, Rodríguez-Sánchez H, Castro JAF (2017) Rhopalurus Junceus scorpion venom induces apoptosis in the triple negative human breast cancer cell line MDA-MB-231. J Venom Res 8:9

PubMed  PubMed Central  Google Scholar 

Ding AH, Nathan CF, Stuehr D (1988) Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol 141(7):2407–2412

Article  CAS  PubMed  Google Scholar 

Ding J, Chua P-J, Bay B-H, Gopalakrishnakone P (2014) Scorpion venoms as a potential source of novel cancer therapeutic compounds. Experimental Biology Med 239(4):387–393

Article  Google Scholar 

Duval K, Grover H, Han L-H, Mou Y, Pegoraro AF, Fredberg J et al (2017) Modeling physiological events in 2D vs. 3D cell culture. Physiology 32(4):266–277

Article  CAS  PubMed  PubMed Central  Google Scholar 

Edmondson R, Broglie JJ, Adcock AF, Yang L (2014) Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay Drug Dev Technol 12(4):207–218

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ghosh S, Pulinilkunnil T, Yuen G, Kewalramani G, An D, Qi D et al (2005) Cardiomyocyte apoptosis induced by short-term diabetes requires mitochondrial GSH depletion. Am J Physiol Heart Circ Physiol 289(2):H768–H76

Article  CAS  PubMed  Google Scholar 

Gomes A, Bhattacharjee P, Mishra R, Biswas AK, Dasgupta SC, Giri B et al (2010) Anticancer potential of animal venoms and toxins

Gupta SD, Gomes A, Debnath A, Saha A, Gomes AJC-bi (2010a) Apoptosis induction in human leukemic cells by a novel protein Bengalin, isolated from Indian black scorpion venom: through mitochondrial pathway and inhibition of heat shock proteins. 183(2):293–303

Gupta SD, Gomes A, Debnath A, Saha A, Gomes A (2010b) Apoptosis induction in human leukemic cells by a novel protein Bengalin, isolated from Indian black scorpion venom: through mitochondrial pathway and inhibition of heat shock proteins. Chemico-Biol Interact 183(2):293–303

Article  Google Scholar 

Jäger H, Dreker T, Buck A, Giehl K, Gress T, Grissmer SJM (2004) Blockage of intermediate-conductance Ca2+-activated K + channels inhibit human pancreatic cancer cell growth in vitro. 65(3):630–638

Jiang X, Zheng D, Lin J (2004) Effects of nitric oxide on mitochondrial permeability transition and cytochrome C of human hepatocellular carcinoma cell lines. Zhongguo Yi Xue Ke Xue Yuan xue bao. Acta Academiae Medicinae Sinicae 26(5):519–523

CAS  PubMed  Google Scholar 

Kapałczyńska M, Kolenda T, Przybyła W, Zajączkowska M, Teresiak A, Filas V et al (2018) 2D and 3D cell cultures–a comparison of different types of cancer cell cultures. Archives Med Science: AMS 14(4):910

Google Scholar 

Karikas G (2010) Anticancer and chemopreventing natural products: some biochemical and therapeutic aspects. J BUON 15(4):627–638

PubMed  Google Scholar 

Keshavarz Alikhani H, Bidmeshkipour A, Zargan J (2020) Cytotoxic and apoptotic induction effects of the venom of Iranian scorpion (Odontobuthus Bidentatus) in the Hepatocellular carcinoma cell line (HepG2). Int J Pept Res Ther 26:2475–2484

Article  CAS  Google Scholar 

Ketterer B (1988) Protective role of glutathione and glutathione transferases in mutagenesis and carcinogenesis. Mutat Research/Fundamental Mol Mech Mutagen 202(2):343–361

Article  CAS  Google Scholar 

Khemili D, Laraba-Djebari F, Hammoudi-Triki D (2020) Involvement of toll-like receptor 4 in neutrophil-mediated inflammation, oxidative stress and tissue damage induced by scorpion venom. Inflammation 43(1):155–167

Article  CAS  PubMed  Google Scholar 

Klöck G, Frank H, Houben R, Zekorn T, Horcher A, Siebers U et al (1994) Production of purified alginates suitable for use in immunoisolated transplantation. 40(5):638–643

Kruger NJ (2009) The Bradford method for protein quantitation. The protein protocols handbook: Springer; pp. 17–24

Lee KY (2012) Mooney DJJPips. Alginate: Prop Biomedical Appl 37(1):106–126

CAS  Google Scholar 

Lourenco WR, Pezier A (2002) Taxonomic consideration of the genus Odontobuthus Vachon (Scorpiones, Buthidae), with description of a new species. Rev Suisse Zool 109(1):115–125

Article  Google Scholar 

Lv D, Hu Z, Lu L, Lu H, Xu X (2017) Three–dimensional cell culture: a powerful tool in tumor research and drug discovery. Oncol Lett 14(6):6999–7010

PubMed  PubMed Central  Google Scholar 

Masihipour B, Zare A, Navidpour S, Taghavi Moghadam A, Akbari A, Rabiei H (2009) Identification and purification of the three toxins from Odotobuthus Bidentatus, Lourenco and Pezier 2002, venom (scorpions, Buthidae). J Bio Sci 9:494–498

Article  CAS  Google Scholar 

Morrison R, Schleicher SM, Sun Y, Niermann KJ, Kim S, Spratt DE et al (2011) Targeting the mechanisms of resistance to chemotherapy and radiotherapy with the cancer stem cell hypothesis. Journal of oncology. 2011

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63

Article  CAS  PubMed  Google Scholar 

Mouhat S, Jouirou B, Mosbah A, De Waard M, Sabatier J-M (2004) Diversity of folds in animal toxins acting on ion channels. Biochem J 378(3):717–726

Article  CAS  PubMed  PubMed Central  Google Scholar 

Olivier C, Oliver L, Lalier L, Vallette FM (2021) Drug resistance in glioblastoma: the two faces of oxidative stress. Front Mol Biosci 7:468

Article  Google Scholar 

Ortiz E, Gurrola GB, Schwartz EF, Possani LD (2015) Scorpion venom components as potential candidates for drug development. Toxicon 93:125–135

Article  CAS  PubMed  Google Scholar 

Övey I, Naziroğlu M (2015) Homocysteine and cytosolic GSH depletion induce apoptosis and oxidative toxicity through cytosolic calcium overload in the hippocampus of aged mice: involvement of TRPM2 and TRPV1 channels. Neuroscience 284:225–233

Article  PubMed  Google Scholar 

Repetto G, Del Peso A, Zurita JL (2008) Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc 3(7):1125

Article  CAS  PubMed  Google Scholar 

Salehi_Najafabadi Z, Goudarzi H, Sajadi M (2021) Evaluation of in vivo lethality and in vitro cytotoxic effect of Odontobuthus Bidentatus scorpion venom. Archives of Razi Institute

Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205

Article  CAS  PubMed  Google Scholar 

Shapiro L, Cohen SJB (1997) Novel alginate sponges for cell culture and transplantation. 18(8):583–590

Shehzad A, Ravinayagam V, AlRumaih H, Aljafary M, Almohazey D, Almofty S et al (2019) Application of three-dimensional (3D) tumor cell culture systems and mechanism of drug resistance. Curr Pharm Design 25(34):3599–3607

Article  CAS  Google Scholar 

Siegel RL, Miller KD, Jemal A (2019) Cancer statistics, 2019. Cancer J Clin 69(1):7–34

Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47(2):389–394

Article  CAS  PubMed  Google Scholar 

Snyder LR, Kirkland JJ, Glajch JL, Practical (2012) HPLC method development: Wiley

Stock AA, Manzoli V, De Toni T, Abreu MM, Poh Y-C, Ye L et al (2020) Conformal coating of stem cell-derived islets for β cell replacement in type 1 diabetes. Stem cell Rep 14(1):91–104

Article  CAS  Google Scholar 

Sun J-Y, Zhao X, Illeperuma WR, Chaudhuri O, Oh KH, Mooney DJ et al (2012) Highly Stretchable Tough Hydrogels 489(7414):133–136

CAS 

Comments (0)

No login
gif