Nuwer R. US agency seeks to phase out animal testing. Nature. 2022. https://doi.org/10.1038/d41586-022-03569-.

Article  PubMed  Google Scholar 

Wadman M. FDA no longer has to require animal testing for new drugs. Science. 2023;379(6628):127–8.

Article  CAS  PubMed  Google Scholar 

Global Cancer Observatory: Cancer Today. Lyon, France: International Agency for Research on Cancer. https://gco.iarc.fr/today. Accessed 2 Dec 2022.

Arnold M, Abnet CC, Neale RE, Vignat J, Giovannucci EL, McGlynn KA, Bray F. Global burden of 5 major types of gastrointestinal cancer. Gastroenterology. 2020;159(1):335–349315.

Article  PubMed  Google Scholar 

Wang DK, Zuo Q, He QY, Li B. Targeted immunotherapies in gastrointestinal cancer: from molecular mechanisms to implications. Front Immunol. 2021;12:705999.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dahiya DS, Kichloo A, Singh J, Albosta M, Lekkala M. Current immunotherapy in gastrointestinal malignancies a review. J Investig Med. 2021;69(3):689–96.

Article  PubMed  Google Scholar 

Dagogo-Jack I, Shaw AT. Tumour heterogeneity and resistance to cancer therapies. Nat Rev Clin Oncol. 2018;15(2):81–94.

Article  CAS  PubMed  Google Scholar 

Wen H, Wang HY, He X, Wu CI. On the low reproducibility of cancer studies. Natl Sci Rev. 2018;5(5):619–24.

Article  PubMed  Google Scholar 

Kaelin WG Jr. Common pitfalls in preclinical cancer target validation. Nat Rev Cancer. 2017;17(7):425–40.

Article  CAS  PubMed  Google Scholar 

Aziz F, Yang X, Wen Q, Yan Q. A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues. Mol Med Rep. 2015;12(2):2939–44.

Article  CAS  PubMed  Google Scholar 

Miserocchi G, Mercatali L, Liverani C, De Vita A, Spadazzi C, Pieri F, Bongiovanni A, Recine F, Amadori D, Ibrahim T. Management and potentialities of primary cancer cultures in preclinical and translational studies. J Transl Med. 2017;15(1):229.

Article  PubMed  PubMed Central  Google Scholar 

Hirsch D, Seyfried S, Staib T, Fiedler D, Sauer C, Ried T, Witt S, Rueckert F, Gaiser T. Newly established gastrointestinal cancer cell lines retain the genomic and immunophenotypic landscape of their parental cancers. Sci Rep. 2020;10(1):17895.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fan H, Demirci U, Chen P. Emerging organoid models: leaping forward in cancer research. J Hematol Oncol. 2019;12(1):142.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gillet JP, Calcagno AM, Varma S, Marino M, Green LJ, Vora MI, Patel C, Orina JN, Eliseeva TA, Singal V, et al. Redefining the relevance of established cancer cell lines to the study of mechanisms of clinical anti-cancer drug resistance. Proc Natl Acad Sci USA. 2011;108(46):18708–13.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Noben M, Vanhove W, Arnauts K, Santo Ramalho A, Van Assche G, Vermeire S, Verfaillie C, Ferrante M. Human intestinal epithelium in a dish: current models for research into gastrointestinal pathophysiology. United European Gastroenterol J. 2017;5(8):1073–81.

Article  PubMed  PubMed Central  Google Scholar 

Bleijs M, van de Wetering M, Clevers H, Drost J. Xenograft and organoid model systems in cancer research. EMBO J. 2019;38(15):e101654.

Article  PubMed  PubMed Central  Google Scholar 

Dieter SM, Giessler KM, Kriegsmann M, Dubash TD, Mohrmann L, Schulz ER, Siegl C, Weber S, Strakerjahn H, Oberlack A, et al. Patient-derived xenografts of gastrointestinal cancers are susceptible to rapid and delayed B-lymphoproliferation. Int J Cancer. 2017;140(6):1356–63.

Article  CAS  PubMed  Google Scholar 

Zeng M, Pi C, Li K, Sheng L, Zuo Y, Yuan J, Zou Y, Zhang X, Zhao W, Lee RJ, et al. Patient-derived xenograft: a more standard “avatar” model in preclinical studies of gastric cancer. Front Oncol. 2022;12:898563.

Article  PubMed  PubMed Central  Google Scholar 

De Angelis ML, Francescangeli F, Nicolazzo C, Xhelili E, La Torre F, Colace L, Bruselles A, Macchia D, Vitale S, Gazzaniga P, et al. An orthotopic patient-derived xenograft (PDX) model allows the analysis of metastasis-associated features in colorectal cancer. Front Oncol. 2022;12:869485.

Article  PubMed  PubMed Central  Google Scholar 

Ben-David U, Ha G, Tseng YY, Greenwald NF, Oh C, Shih J, McFarland JM, Wong B, Boehm JS, Beroukhim R, et al. Patient-derived xenografts undergo mouse-specific tumor evolution. Nat Genet. 2017;49(11):1567–75.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Durinikova E, Buzo K, Arena S. Preclinical models as patients’ avatars for precision medicine in colorectal cancer: past and future challenges. J Exp Clin Cancer Res. 2021;40(1):185.

Article  PubMed  PubMed Central  Google Scholar 

Weeber F, van de Wetering M, Hoogstraat M, Dijkstra KK, Krijgsman O, Kuilman T, Gadellaa-van Hooijdonk CG, van der Velden DL, Peeper DS, Cuppen EP, et al. Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases. Proc Natl Acad Sci USA. 2015;112(43):13308–11.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Morton CL, Houghton PJ. Establishment of human tumor xenografts in immunodeficient mice. Nat Protoc. 2007;2(2):247–50.

Article  CAS  PubMed  Google Scholar 

Gunti S, Hoke ATK, Vu KP, London NR Jr. Organoid and spheroid tumor models: techniques and applications. Cancers. 2021;13(4):874.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Weiswald LB, Richon S, Validire P, Briffod M, Lai-Kuen R, Cordelieres FP, Bertrand F, Dargere D, Massonnet G, Marangoni E, et al. Newly characterised ex vivo colospheres as a three-dimensional colon cancer cell model of tumour aggressiveness. Br J Cancer. 2009;101(3):473–82.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Balmana M, Mereiter S, Diniz F, Feijao T, Barrias CC, Reis CA. Multicellular human gastric-cancer spheroids mimic the glycosylation phenotype of gastric carcinomas. Molecules. 2018;23(11):2815.

Article  PubMed  PubMed Central  Google Scholar 

Wang J, Zhang X, Li X, Zhang Y, Hou T, Wei L, Qu L, Shi L, Liu Y, Zou L, et al. Anti-gastric cancer activity in three-dimensional tumor spheroids of bufadienolides. Sci Rep. 2016;6:24772.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang H, Qin Y, Jia M, Li L, Zhang W, Li L, Zhang Z, Liu Y. A gastric cancer patient-derived three-dimensional cell spheroid culture model. Am J Cancer Res. 2023;13(3):964–75.

CAS  PubMed  PubMed Central  Google Scholar 

Nagle PW, Plukker JTM, Muijs CT, van Luijk P, Coppes RP. Patient-derived tumor organoids for prediction of cancer treatment response. Semin Cancer Biol. 2018;53:258–64.

Article  CAS  PubMed  Google Scholar 

Jeppesen M, Hagel G, Glenthoj A, Vainer B, Ibsen P, Harling H, Thastrup O, Jorgensen LN, Thastrup J. Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine. PLoS ONE. 2017;12(9):e0183074.

Article  PubMed  PubMed Central  Google Scholar 

Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, van Es JH, Abo A, Kujala P, Peters PJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459(7244):262–5.

Article  CAS  PubMed  Google Scholar 

Rossi G, Manfrin A, Lutolf MP. Progress and potential in organoid research. Nat Rev Genet. 2018;19(11):671–87.

Article  CAS  PubMed  Google Scholar 

Li G, Ma S, Wu Q, Kong D, Yang Z, Gu Z, Feng L, Zhang K, Cheng S, Tian Y, et al. Establishment of gastric signet ring cell carcinoma organoid for the therapeutic drug testing. Cell Death Discov. 2022;8(1):6.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ohta Y, Sato T. Intestinal tumor in a dish. Front Med. 2014;1:14.

Article  Google Scholar 

Walsh AJ, Cook RS, Sanders ME, Arteaga CL, Skala MC. Drug response in organoids generated from frozen primary tumor tissues. Sci Rep. 2016;6:18889.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Alzeeb G, Metges JP, Corcos L, Le Jossic-Corcos C. Three-dimensional culture systems in gastric cancer research. Cancers. 2020;12(10):2800.

Article  CAS