Hou X, Zaks T, Langer R, Dong Y. Lipid nanoparticles for mRNA delivery. Nat Rev Mater. 2021;6(12):1078–94.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Huang X, Kong N, Zhang X, Cao Y, Langer R, Tao W. The landscape of mRNA nanomedicine. Nat Med. 2022;11:2273–87.

Article  Google Scholar 

Gjetting T, Arildsen NS, Christensen CL, Poulsen TT, Roth JA, Handlos VN, Poulsen HS. In vitro and in vivo effects of polyethylene glycol (PEG)-modified lipid in DOTAP/cholesterol-mediated gene transfection. Int J Nanomedicine. 2010;5:371–83.

PubMed  PubMed Central  CAS  Google Scholar 

Ibrahim M, Ramadan E, Elsadek NE, Emam SE, Shimizu T, Ando H, Ishima Y, Elgarhy OH, et al. Polyethylene glycol (PEG): The nature, immunogenicity, and role in the hypersensitivity of PEGylated products. J Control Release. 2022;351:215–30.

Article  PubMed  CAS  Google Scholar 

Knop K, Hoogenboom R, Fischer D, Schubert US. Poly(ethylene glycol) in drug delivery: pros and cons as well as potential alternatives. Angew Chem Int Ed Engl. 2010;49(36):6288–308.

Article  PubMed  CAS  Google Scholar 

Suzuki T, Suzuki Y, Hihara T, Kubara K, Kondo K, Hyodo K, Yamazaki K, Ishida T, et al. PEG shedding-rate-dependent blood clearance of PEGylated lipid nanoparticles in mice: Faster PEG shedding attenuates anti-PEG IgM production. Int J Pharm. 2020;588: 119792.

Article  PubMed  CAS  Google Scholar 

Lin C, Jans A, Wolters JC, Mohamed MR, Van der Vorst EPC, Trautwein C, Bartneck M. Targeting ligand independent tropism of siRNA-LNP by small molecules for directed therapy of liver or myeloid immune cells. Adv Healthc Mater. 2024;13(26): e2202670.

PubMed  Google Scholar 

Zhu S, Tang Y, Lin C, Liu XY, Lin Y. Recent advances in patterning natural polymers: From nanofabrication techniques to applications. Small Methods. 2021;5(3): e2001060.

Article  PubMed  Google Scholar 

Ghezzi M, Pescina S, Padula C, Santi P, Del Favero E, Cantù L, Nicoli S. Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions. J Control Release. 2021;332:312–36.

Article  PubMed  CAS  Google Scholar 

Li T, Shi S, Goel S, Shen X, Xie X, Chen Z, Zhang H, Li S, et al. Recent advancements in mesoporous silica nanoparticles towards therapeutic applications for cancer. Acta Biomater. 2019;89:1–13.

Article  PubMed  CAS  Google Scholar 

Hajji S, Younes I, Ghorbel-Bellaaj O, Hajji R, Rinaudo M, Nasri M, Jellouli K. Structural differences between chitin and chitosan extracted from three different marine sources. Int J Biol Macromol. 2014;65:298–306.

Article  PubMed  CAS  Google Scholar 

Li J, Wang J, Chen Z. Emerging role of exosomes in cancer therapy: progress and challenges. Mol Cancer. 2025;24(1):13.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Mashouri L, Yousefi H, Aref AR, Ahadi AM, Molaei F, Alahari SK. Exosomes: composition, biogenesis, and mechanisms in cancer metastasis and drug resistance. Mol Cancer. 2019;18(1):75.

Article  PubMed  PubMed Central  Google Scholar 

Liu M, Wang Y, Zhang Y, Hu D, Tang L, Zhou B, Yang L. Landscape of small nucleic acid therapeutics: moving from the bench to the clinic as next-generation medicines. Signal Transduct Target Ther. 2025;10(1):73.

Article  PubMed  PubMed Central  Google Scholar 

Ahn SH, Ryu SW, Choi H, You S, Park J, Choi C. Manufacturing Therapeutic Exosomes: from Bench to Industry. Mol Cells. 2022;45(5):284–90.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Cai W, He D. Bone marrow mesenchymal stem cell-derived exosomes improve cancer drug delivery in human cell lines and a mouse osteosarcoma model. Front Oncol. 2024;14:1482087.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Tenchov R, Bird R, Curtze AE, Zhou Q. Lipid nanoparticles ─ From liposomes to mRNA vaccine delivery, a landscape of research diversity and advancement. ACS Nano. 2021;15(11):16982–7015.

Article  PubMed  CAS  Google Scholar 

Inglut CT, Sorrin AJ, Kuruppu T, Vig S, Cicalo J, Ahmad H, Huang HC. Immunological and toxicological considerations for the design of liposomes. Nanomaterials (Basel). 2020;10(2):190.

Article  PubMed  CAS  Google Scholar 

Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V. A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol. 2010;40(4):328–46.

Article  PubMed  CAS  Google Scholar 

Petrarca C, Clemente E, Amato V, Pedata P, Sabbioni E, Bernardini G, Iavicoli I, Cortese S, et al. Engineered metal based nanoparticles and innate immunity. Clin Mol Allergy. 2015;13(1):13.

Article  PubMed  PubMed Central  Google Scholar 

Norouzi M, Yathindranath V, Thliveris JA, Kopec BM, Siahaan TJ, Miller DW. Doxorubicin-loaded iron oxide nanoparticles for glioblastoma therapy: a combinational approach for enhanced delivery of nanoparticles. Sci Rep. 2020;10(1):11292.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Mohammadpour R, Yazdimamaghani M, Cheney DL, Jedrzkiewicz J, Ghandehari H. Subchronic toxicity of silica nanoparticles as a function of size and porosity. J Control Release. 2019;304:216–32.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Kobayashi N, Izumi H, Morimoto Y. Review of toxicity studies of carbon nanotubes. J Occup Health. 2017;59(5):394–407.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Meng J, Yang M, Jia F, Kong H, Zhang W, Wang C, Xing J, Xie S, Xu H. Subcutaneous injection of water-soluble multi-walled carbon nanotubes in tumor-bearing mice boosts the host immune activity. Nanotechnology. 2010;21(14): 145104.

Article  PubMed  Google Scholar 

Morimoto Y, Horie M, Kobayashi N, Shinohara N, Shimada M. Inhalation toxicity assessment of carbon-based nanoparticles. Acc Chem Res. 2013;46(3):770–81.

Article  PubMed  CAS  Google Scholar 

Abulikemu A, Zhao X, Xu H, Li Y, Ma R, Yao Q, Wang J, Sun Z, et al. Silica nanoparticles aggravated the metabolic associated fatty liver disease through disturbed amino acid and lipid metabolisms-mediated oxidative stress. Redox Biol. 2023;59: 102569.

Article  PubMed  CAS  Google Scholar 

Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, Liebler DC, Ping J, et al. Reassessment of exosome composition. Cell. 2019;177(2):428-445.e18.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Prunevieille A, Babiker-Mohamed MH, Aslami C, Gonzalez-Nolasco B, Mooney N, Benichou G. T cell antigenicity and immunogenicity of allogeneic exosomes. Am J Transplant. 2021;21(7):2583–9.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Kou M, Huang L, Yang J, Chiang Z, Chen S, Liu J, Guo L, Zhang X, et al. Mesenchymal stem cell-derived extracellular vesicles for immunomodulation and regeneration: a next generation therapeutic tool? Cell Death Dis. 2022;13(7):580.

Article  PubMed  PubMed Central  CAS  Google Scholar 

LeBleu VS, Smaglo BG, Mahadevan KK, Kirtley ML, McAndrews KM, Mendt M, Yang S, Maldonado AS, et al. KRAS G12D-specific targeting with engineered exosomes reprograms the immune microenvironment to enable efficacy