and Serve as Essential Gatekeepers for Monitoring In Vitro Production of Pro-inflammatory Cytokines in THP-1 Monocytic Cells

Becher B, Spath S, Goverman J. Cytokine networks in neuroinflammation. Nat Rev Immunol. 2016. https://doi.org/10.1038/nri.2016.123.

Article  PubMed  Google Scholar 

Barnes PJ. The cytokine network in asthma and chronic obstructive pulmonary disease. J Clin Investig. 2008;118(11):3546–56. https://doi.org/10.1172/JCI36130.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gulati K, Guhathakurta S, Joshi J, Rai N, Ray A. Cytokines and their role in health and disease: a brief overview. MOJ Immunol. 2016;4(2):00121. https://doi.org/10.15406/MOJI.2016.04.00121.

Article  Google Scholar 

Zhou Y, Hong Y, Huang H. Triptolide attenuates inflammatory response in membranous glomerulo-nephritis rat via downregulation of NF-κB signaling pathway. Kidney Blood Press Res. 2016;41(6):901–10. https://doi.org/10.1159/000452591.

Article  CAS  PubMed  Google Scholar 

Glass CK, Saijo K, Winner B, Marchetto MC, Gage FH. Mechanisms underlying inflammation in neurodegeneration. Cell. 2010;140(6):918–34. https://doi.org/10.1016/J.CELL.2010.02.016.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mishra A, Kim HJ, Shin AH, Thayer SA. Synapse loss induced by interleukin-1β requires pre- and post-synaptic mechanisms. J Neuroimmune Pharmacol. 2012;7(3):571–8. https://doi.org/10.1007/S11481-012-9342-7.

Article  PubMed  PubMed Central  Google Scholar 

Lopez-Ramirez MA, Fischer R, Torres-Badillo CC, Davies HA, Logan K, Pfizenmaier K, Male DK, Sharrack B, Romero IA. Role of caspases in cytokine-induced barrier breakdown in human brain endothelial cells. J Immunol. 2012;189(6):3130–9. https://doi.org/10.4049/JIMMUNOL.1103460.

Article  CAS  PubMed  Google Scholar 

Rochfort KD, Cummins PM. The blood–brain barrier endothelium: a target for pro-inflammatory cytokines. Biochem Soc Trans. 2015;43(4):702–6. https://doi.org/10.1042/BST20140319.

Article  CAS  PubMed  Google Scholar 

Paradkar PH, Joshi JV, Mertia PN, Agashe SV, Vaidya RA. Role of cytokines in genesis, progression and prognosis of cervical cancer. Asian Pac J Cancer Prev APJCP. 2014;15(9):3851–64. https://doi.org/10.7314/APJCP.2014.15.9.3851.

Article  PubMed  Google Scholar 

Lynch L, Nowak M, Varghese B, Clark J, Hogan AE, Toxavidis V, Balk SP, O’Shea D, O’Farrelly C, Exley MA. Adipose tissue invariant NKT cells protect against diet-induced obesity and metabolic disorder through regulatory cytokine production. Immunity. 2012;37(3):574–87. https://doi.org/10.1016/J.IMMUNI.2012.06.016.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shi J, Fan J, Su Q, Yang Z. Cytokines and abnormal glucose and lipid metabolism. Front Endocrinol. 2019;10:703. https://doi.org/10.3389/FENDO.2019.00703.

Article  Google Scholar 

Chanput W, Mes JJ, Wichers HJ. THP-1 cell line: an in vitro cell model for immune modulation approach. Int Immunopharmacol. 2014;23(1):37–45. https://doi.org/10.1016/J.INTIMP.2014.08.002.

Article  CAS  PubMed  Google Scholar 

Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer. 1980;26(2):171–6. https://doi.org/10.1002/IJC.2910260208.

Article  CAS  PubMed  Google Scholar 

Daigneault M, Preston JA, Marriott HM, Whyte MKB, Dockrell DH. The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS ONE. 2010;5(1):e8668. https://doi.org/10.1371/JOURNAL.PONE.0008668.

Article  PubMed  PubMed Central  Google Scholar 

Baxter EW, Graham AE, Re NA, Carr IM, Robinson JI, Mackie SL, Morgan AW. Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes. J Immunol Methods. 2020;478:112721. https://doi.org/10.1016/J.JIM.2019.112721.

Article  CAS  PubMed  Google Scholar 

Park EK, Jung HS, Yang HI, Yoo MC, Kim C, Kim KS. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 2007;56(1):45–50. https://doi.org/10.1007/S00011-007-6115-5.

Article  CAS  PubMed  Google Scholar 

Product Information| RIPA Buffer 16488-34 100ML Nacalai Tesque Inc.| e-Nacalai Search Version. (n.d.). Retrieved December 10, 2023, from https://www.e-nacalai.jp/ComDocs/Manual/16488-1-E.pdf.

Bis-Tris Gel Recipes| Corson Lab| IU School of Medicine. (n.d.). Retrieved September 14, 2023, from https://medicine.iu.edu/faculty-labs/corson/protocols/gel-recipes.

Spiller KL, Wrona EA, Romero-Torres S, Pallotta I, Graney PL, Witherel CE, Panicker LM, Feldman RA, Urbanska AM, Santambrogio L, Vunjak-Novakovic G, Freytes DO. Differential gene expression in human, murine, and cell line-derived macrophages upon polarization. Exp Cell Res. 2016;347(1):1–13. https://doi.org/10.1016/J.YEXCR.2015.10.017.

Article  CAS  PubMed  Google Scholar 

Tedesco S, Bolego C, Toniolo A, Nassi A, Fadini GP, Locati M, Cignarella A. Phenotypic activation and pharmacological outcomes of spontaneously differentiated human monocyte-derived macrophages. Immunobiology. 2015;220(5):545–54. https://doi.org/10.1016/J.IMBIO.2014.12.008.

Article  CAS  PubMed  Google Scholar 

Mia S, Warnecke A, Zhang XM, Malmström V, Harris RA. An optimized protocol for human M2 macrophages using M-CSF and IL-4/IL-10/TGF-β yields a dominant immunosuppressive phenotype. Scand J Immunol. 2014;79(5):305–14. https://doi.org/10.1111/SJI.12162.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Namgaladze D, Snodgrass RG, Angioni C, Grossmann N, Dehne N, Geisslinger G, Brüne B. AMP-activated protein kinase suppresses arachidonate 15-lipoxygenase expression in interleukin 4-polarized human macrophages. J Biol Chem. 2015;290(40):24484–94. https://doi.org/10.1074/JBC.M115.678243.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kohro T, Tanaka T, Murakami T, Wada Y, Aburatani H, Hamakubo T, Kodama T. A comparison of differences in the gene expression profiles of phorbol 12-myristate 13-acetate differentiated THP-1 cells and human monocyte-derived macrophage. J Atheroscler Thromb. 2004;11(2):88–97. https://doi.org/10.5551/JAT.11.88.

Article  CAS  PubMed  Google Scholar 

Sharif O, Bolshakov VN, Raines S, Newham P, Perkins ND. Transcriptional profiling of the LPS induced NF-κB response in macrophages. BMC Immunol. 2007;8:1–17. https://doi.org/10.1186/1471-2172-8-1.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Maeß MB, Wittig B, Cignarella A, Lorkowski S. Reduced PMA enhances the responsiveness of transfected THP-1 macrophages to polarizing stimuli. J Immunol Methods. 2014;402(1–2):76–81. https://doi.org/10.1016/J.JIM.2013.11.006.

Article  PubMed  Google Scholar 

Lund ME, To J, O’Brien BA, Donnelly S. The choice of phorbol 12-myristate 13-acetate differentiation protocol influences the response of THP-1 macrophages to a pro-inflammatory stimulus. J Immunol Methods. 2016;430:64–70. https://doi.org/10.1016/J.JIM.2016.01.012.

Article  CAS  PubMed  Google Scholar 

Das R, Ganapathy S, Mahabeleshwar GH, Drumm C, Febbraio M, Jain MK, Plow EF. Macrophage gene expression and foam cell formation are regulated by plasminogen. Circulation. 2013;127(11):1209–18. https://doi.org/10.1161/CIRCULATIONAHA.112.001214.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bouckenooghe T, Sisino G, Aurientis S, Chinetti-Gbaguidi G, Kerr-Conte J, Staels B, Fontaine P, Storme L, Pattou F, Vambergue A. Adipose tissue macrophages (ATM) of obese patients are releasing increased levels of prolactin during an inflammatory challenge: a role for prolactin in diabesity? Biochem Biophys Acta. 2014;1842(4):584–93. https://doi.org/10.1016/J.BBADIS.2013.12.005.

Article  CAS  PubMed  Google Scholar 

Lin Y, Huang M, Wang S, You X, Zhang L, Chen Y. PAQR11 modulates monocyte-to-macrophage differentiation and pathogenesis of rheumatoid arthritis. Immunology. 2021;163(1):60–73. https://doi.org/10.1111/IMM.13303.

Comments (0)

No login
gif