In vivo thrombin activity in the diatom Phaeodactylum tricornutum: biotechnological insights

Allen AE, Vardi A, Bowler C (2006) An ecological and evolutionary context for integrated nitrogen metabolism and related signaling pathways in marine diatoms. Curr Opin Plant Biol 9(3):264–273. https://doi.org/10.1016/j.pbi.2006.03.013

Article  PubMed  CAS  Google Scholar 

Amet N, Lee HF, Shen WC (2009) Insertion of the designed helical linker led to increased expression of tf-based fusion proteins. Pharm Res 26(3):523–528. https://doi.org/10.1007/s11095-008-9767-0

Article  PubMed  CAS  Google Scholar 

Araujo AP, Oliva G, Henrique-Silva F, Garratt RC, Caceres O, Beltramini LM (2000) Influence of the histidine tail on the structure and activity of recombinant chlorocatechol 1,2-dioxygenase. Biochem Biophys Res Commun 272(2):480–484. https://doi.org/10.1006/bbrc.2000.2802

Article  PubMed  CAS  Google Scholar 

Argos P (1990) An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion. J Mol Biol 211(4):943–958. https://doi.org/10.1016/0022-2836(90)90085-Z

Article  PubMed  CAS  Google Scholar 

Awwad F, Fantino EI, Héneault M, Diaz-Garza AM, Merindol N, Custeau A, Gélinas S-E, Meddeb-Mouelhi F, Li J, Lemay J-F, Karas BJ, Desgagne-Penix I (2023) Bioengineering of the marine diatom Phaeodactylum tricornutum with Cannabis genes enables the production of the cannabinoid precursor, olivetolic acid. Int J Mol Sci.  https://doi.org/10.3390/ijms242316624

Belardinelli JM, Jackson M (2017) Green fluorescent protein as a protein localization and topological reporter in mycobacteria. Tuberculosis (Edinb) 105:13–17. https://doi.org/10.1016/j.tube.2017.04.001

Article  PubMed  CAS  Google Scholar 

Bhandari M, Ofosu FA, Mackman N, Jackson C, Doria C, Humphries JE, Babu SC, Ortel TL, Hoffman Van Thiel D, Walenga JM, Wahi R, Teoh KH, Fareed J (2011) Safety and efficacy of thrombin-JMI: a multidisciplinary expert group consensus. Clin Appl Thromb Hemost 17(1):39–45. https://doi.org/10.1177/1076029610385674

Article  PubMed  Google Scholar 

Bode W, Mayr I, Baumann U, Huber R, Stone SR, Hofsteenge J (1989) The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment. EMBO J 8(11):3467–3475. https://doi.org/10.1002/j.1460-2075.1989.tb08511.x

Article  PubMed  PubMed Central  CAS  Google Scholar 

Bouabe H, Fassler R, Heesemann J (2008) Improvement of reporter activity by IRES-mediated polycistronic reporter system. Nucleic Acids Res 36(5):e28. https://doi.org/10.1093/nar/gkm1119

Article  PubMed  PubMed Central  CAS  Google Scholar 

Bowler C, Allen AE, Badger JH, Grimwood J, Jabbari K, Kuo A, Maheswari U, Martens C, Maumus F, Otillar RP, Rayko E, Salamov A, Vandepoele K, Beszteri B, Gruber A, Heijde M, Katinka M, Mock T, Valentin K, Verret F, Berges JA, Brownlee C, Cadoret JP, Chiovitti A, Choi CJ, Coesel S, De Martino A, Detter JC, Durkin C, Falciatore A, Fournet J, Haruta M, Huysman MJ, Jenkins BD, Jiroutova K, Jorgensen RE, Joubert Y, Kaplan A, Kroger N, Kroth PG, La Roche J, Lindquist E, Lommer M, Martin-Jezequel V, Lopez PJ, Lucas S, Mangogna M, McGinnis K, Medlin LK, Montsant A, Oudot-Le Secq MP, Napoli C, Obornik M, Parker MS, Petit JL, Porcel BM, Poulsen N, Robison M, Rychlewski L, Rynearson TA, Schmutz J, Shapiro H, Siaut M, Stanley M, Sussman MR, Taylor AR, Vardi A, von Dassow P, Vyverman W, Willis A, Wyrwicz LS, Rokhsar DS, Weissenbach J, Armbrust EV, Green BR, Van de Peer Y, Grigoriev IV (2008) The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature 456(7219):239–244. https://doi.org/10.1038/nature07410

Article  PubMed  CAS  Google Scholar 

Butler T, Kapoore RV, Vaidyanathan S (2020) Phaeodactylum tricornutum: a diatom cell factory. Trends Biotechnol 38(6):606–622. https://doi.org/10.1016/j.tibtech.2019.12.023

Article  PubMed  CAS  Google Scholar 

Castellino FJ, McCance SG (1997) The kringle domains of human plasminogen. Ciba Found Symp 212:46–60. https://doi.org/10.1002/9780470515457.ch4

Article  PubMed  CAS  Google Scholar 

Cheng CM, Meyer-Massetti C, Kayser SR (2009) A review of three stand-alone topical thrombins for surgical hemostasis. Clin Ther 31:1. https://doi.org/10.1016/j.clinthera.2009.01.005

Article  Google Scholar 

Coughlin SR (2000) Thrombin signalling and protease-activated receptors. Nature 407:258–264. https://doi.org/10.1038/35025229

Article  PubMed  CAS  Google Scholar 

Danckwardt S, Hentze MW, Kulozik AE (2013) Pathologies at the nexus of blood coagulation and inflammation: thrombin in hemostasis, cancer, and beyond. J Mol Med (Berl) 91(11):1257–1271. https://doi.org/10.1007/s00109-013-1074-5

Article  PubMed  CAS  Google Scholar 

Demain AL, Vaishnav P (2009) Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv 27(3):297–306. https://doi.org/10.1016/j.biotechadv.2009.01.008

Article  PubMed  CAS  Google Scholar 

Dhaouadi F, Awwad F, Diamond A, Desgagné-Penix I (2020) Diatoms’ breakthroughs in biotechnology: Phaeodactylum tricornutum; as a model for producing high-added value molecules. Am J Plant Sci 11(10):1632–1670. https://doi.org/10.4236/ajps.2020.1110118

Article  CAS  Google Scholar 

Di Cera E (2008) Thrombin. Mol Aspects Med 29(4):203–254. https://doi.org/10.1016/j.mam.2008.01.001

Article  PubMed  PubMed Central  CAS  Google Scholar 

Diamond A, Diaz-Garza AM, Li J, Slattery SS, Merindol N, Fantino E, Meddeb-Mouelhi F, Karas BJ, Barnabé S, Desgagné-Penix I (2023) Instability of extrachromosomal DNA transformed into the diatom Phaeodactylum tricornutum. Algal Research 70.  https://doi.org/10.1016/j.algal.2023.102998

Dos Santos TW, Goncalves PA, Rodriguez D, Pereira JA, Martinez CAR, Leite LCC, Ferraz LFC, Converso TR, Darrieux M (2022) A fusion protein comprising pneumococcal surface protein A and a pneumolysin derivate confers protection in a murine model of pneumococcal pneumonia. PLoS ONE 17(12):e0277304. https://doi.org/10.1371/journal.pone.0277304

Article  PubMed  PubMed Central  CAS  Google Scholar 

Ellis RW, Rappuoli R, Ahmed S (2013) Technologies for making new vaccines. In: Vaccines (sixth edition), pp 1182–1199. https://doi.org/10.1016/B978-1-4557-0090-5.00013-6

Erdene-Ochir E, Shin BK, Kwon B, Jung C, Pan CH (2019) Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum. Sci Rep 9(1):9941. https://doi.org/10.1038/s41598-019-45786-9

Article  PubMed  PubMed Central  CAS  Google Scholar 

Fabris M, George J, Kuzhiumparambil U, Lawson CA, Jaramillo-Madrid AC, Abbriano RM, Vickers CE, Ralph P (2020) Extrachromosomal genetic engineering of the marine diatom Phaeodactylum tricornutum enables the heterologous production of monoterpenoids. ACS Synth Biol 9(3):598–612. https://doi.org/10.1021/acssynbio.9b00455

Article  PubMed  CAS  Google Scholar 

Fantino E, Awwad F, Merindol N, Diaz Garza AM, Gélinas S-E, Gajón Robles GC, Custeau A, Meddeb-Mouelhi F, Desgagné-Penix I (2024) Bioengineering Phaeodactylum tricornutum, a marine diatom, for cannabinoid biosynthesis. Algal Res 77:103379. https://doi.org/10.1016/j.algal.2023.103379

Article  Google Scholar 

Freedman SJ, Blostein MD, Baleja JD, Jacobs M, Furie BC, Furie B (1996) Identification of the phospholipid binding site in the vitamin K-dependent blood coagulation protein factor IX. J Biol Chem 271(27):16227–16236. https://doi.org/10.1074/jbc.271.27.16227

Article  PubMed  CAS  Google Scholar 

Fu W, Chaiboonchoe A, Dohai B, Sultana M, Baffour K, Alzahmi A, Weston J, Al Khairy D, Daakour S, Jaiswal A, Nelson DR, Mystikou A, Brynjolfsson S, Salehi-Ashtiani K (2020) GPCR genes as activators of surface colonization pathways in a model marine diatom. iScience 23(8):101424. https://doi.org/10.1016/j.isci.2020.101424

Article  PubMed  PubMed Central  CAS  Google Scholar 

Gallwitz M, Enoksson M, Thorpe M, Hellman L (2012) The extended cleavage specificity of human thrombin. PLoS ONE 7(2):e31756. https://doi.org/10.1371/journal.pone.0031756

Article  PubMed  PubMed Central  CAS  Google Scholar 

George RA, Heringa J (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Eng 15(11):871–879. https://doi.org/10.1093/protein/15.11.871

Article  PubMed  CAS  Google Scholar 

Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E, Crea R et al (1988) Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci U S A 85(16):5879–5883. https://doi.org/10.1073/pnas.85.16.5879

Comments (0)

No login
gif