Rapid quantitative high-throughput mouse embryoid body model for embryotoxicity assessment

Barnes RM, Firulli BA, VanDusen NJ et al (2011) Hand2 loss-of-function in Hand1-expressing cells reveals distinct roles in epicardial and coronary vessel development. Circ Res 108(8):940–949. https://doi.org/10.1161/circresaha.110.233171

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barrier M, Jeffay S, Nichols HP et al (2011) Mouse embryonic stem cell adherent cell differentiation and cytotoxicity (ACDC) assay. Reprod Toxicol 31(4):383–391. https://doi.org/10.1016/j.reprotox.2011.01.007

Article  CAS  PubMed  Google Scholar 

Buesen R, Genschow E, Slawik B et al (2009) Embryonic stem cell test remastered: comparison between the validated EST and the new molecular FACS-EST for assessing developmental toxicity in vitro. Toxicol Sci 108(2):389–400. https://doi.org/10.1093/toxsci/kfp012

Article  CAS  PubMed  Google Scholar 

Chemicals production and consumption statistics (2023) European Union. https://ec.europa.eu/eurostat/statistics-explained/index.php?title=Chemicals_production_and_consumption_statistics&oldid=416142#Total_production_of_chemicals. Accessed 19 Apr 2024

Choy Buentello D, Koch LS, Trujillo-de Santiago G, Alvarez MM, Broersen K (2022) Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies. PLoS ONE 17(5):e0262062. https://doi.org/10.1371/journal.pone.0262062

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dix DJ, Houck KA, Martin MT, Richard AM, Woodrow RR, Setzer RW, Kavlock RJ (2007) The ToxCast Program for Prioritizing Toxicity Testing of Environmental Chemicals. Toxicol Sci 95(1):5–12. https://doi.org/10.1093/toxsci/kfl103

Genschow E, Spielmann H, Scholz G et al (2004) Validation of the embryonic stem cell test in the international ECVAM validation study on three in vitro embryotoxicity tests. Altern Lab Anim 32(3):209–244. https://doi.org/10.1177/026119290403200305

Article  CAS  PubMed  Google Scholar 

George RM, Firulli AB (2019) Hand factors in cardiac development. Anat Rec (Hoboken) 302(1):101–107. https://doi.org/10.1002/ar.23910

Article  PubMed  Google Scholar 

Hinton RB, Yutzey KE, Benson DW (2005) Congenital heart disease: genetic causes and developmental insights. Prog Pediatr Cardiol 20(2):101–111. https://doi.org/10.1016/j.ppedcard.2005.04.002

Article  Google Scholar 

Hwang Y, Chung BG, Ortmann D, Hattori N, Moeller H, Khademhosseini A (2009) Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Proc Natl Acad Sci USA 106(40):16978–16983. https://doi.org/10.1073/pnas.0905550106

Article  PubMed  PubMed Central  Google Scholar 

International Council for Harmonisation, I (2017) ICH S5 (R3) guideline on reproductive toxicology: detection of toxicity to reproduction for human pharmaceuticals (Step 2b)

ICH (2020) Detection of reproductive and developmental toxicity for human pharmaceuticals S5 (R3) Final version in ICH HARMONISED GUIDELINE (ed. ICH Expert Working Group.) 13–110.

Kang HY, Choi YK, Jo NR et al (2017) Advanced developmental toxicity test method based on embryoid body’s area. Reprod Toxicol 72:74–85. https://doi.org/10.1016/j.reprotox.2017.06.185

Article  CAS  PubMed  Google Scholar 

Kim PT, Ong CJ (2012) Differentiation of definitive endoderm from mouse embryonic stem cells. Results Probl Cell Differ 55:303–319. https://doi.org/10.1007/978-3-642-30406-4_17

Article  CAS  PubMed  Google Scholar 

Kowalski MP, Yoder A, Liu L, Pajak L (2012) Controlling embryonic stem cell growth and differentiation by automation: enhanced and more reliable differentiation for drug discovery. J Biomol Screen 17(9):1171–1179. https://doi.org/10.1177/1087057112452783

Article  PubMed  Google Scholar 

Le Coz F, Suzuki N, Nagahori H, Omori T, Saito K (2015) Hand1-Luc embryonic stem cell test (Hand1-Luc EST): a novel rapid and highly reproducible in vitro test for embryotoxicity by measuring cytotoxicity and differentiation toxicity using engineered mouse ES cells. J Toxicol Sci 40(2):251–261. https://doi.org/10.2131/jts.40.251

Article  PubMed  Google Scholar 

Lee J-H, Park SY, Ahn C et al (2019) Pre-validation study of alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies. Food Chem Toxicol 123:50–56. https://doi.org/10.1016/j.fct.2018.10.044

Article  CAS  PubMed  Google Scholar 

Liu Y, Asakura M, Inoue H et al (2007) Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells. Proc Natl Acad Sci 104(10):3859–3864. https://doi.org/10.1073/pnas.0609100104

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu Y, Kaneda R, Leja TW et al (2014) Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. STEM CELLS 32(6):1515–1526. https://doi.org/10.1002/stem.1695

Article  CAS  PubMed  Google Scholar 

Marx-Stoelting P, Adriaens E, Ahr H-J et al (2009) A review of the implementation of the embryonic stem cell test (EST): the report and recommendations of an ECVAM/ReProTect Workshopa. Altern Lab Anim 37(3):313–328. https://doi.org/10.1177/026119290903700314

Article  CAS  PubMed  Google Scholar 

Petersen EJ, Nguyen A, Brown J et al (2021) Characteristics to consider when selecting a positive control material for an in vitro assay. ALTEX Altern Anim Exp 38(2):365–376. https://doi.org/10.14573/altex.2102111

Article  Google Scholar 

Seiler AEM, Spielmann H (2011) The validated embryonic stem cell test to predict embryotoxicity in vitro. Nat Protoc 6(7):961–978. https://doi.org/10.1038/nprot.2011.348

Article  CAS  PubMed  Google Scholar 

Spielmann H (2005) Predicting the risk of developmental toxicity from in vitro assays. Toxicol Appl Pharmacol 207(2, Supplement):375–380. https://doi.org/10.1016/j.taap.2005.01.049

Article  CAS  PubMed  Google Scholar 

Tamura M, Amano T, Shiroishi T (2014) Chapter three—the Hand2 gene dosage effect in developmental defects and human congenital disorders. In: Taneja R (ed) Current topics in developmental biology, vol 110. Academic Press, pp 129–152

Google Scholar 

Tice RR, Austin CP, Kavlock RV, Bucher JR (2013) Improving the human hazard characterization of chemicals: a Tox21 update. Environ Health Perspect 121(7):756–165. https://doi.org/10.1289/ehp.1205784

Varshney A, Chahal G, Santos L et al (2021) Human cardiac transcription factor networks. In: Wolkenhauer O (ed) Systems medicine. Academic Press, Oxford, pp 429–453

Chapter  Google Scholar 

Witt G, Keminer O, Leu J et al (2021) An automated and high-throughput-screening compatible pluripotent stem cell-based test platform for developmental and reproductive toxicity assessment of small molecule compounds. Cell Biol Toxicol 37(2):229–243. https://doi.org/10.1007/s10565-020-09538-0

Article  CAS  PubMed  Google Scholar 

Yan Y-T, Gritsman K, Ding J et al (1999) Conserved requirement for EGF–CFC genes in vertebrate left–right axis formation. Genes Dev 13(19):2527–2537

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhao M, Tang Y, Zhou Y, Zhang J (2019) Deciphering role of Wnt signalling in cardiac mesoderm and cardiomyocyte differentiation from human iPSCs: four-dimensional control of Wnt pathway for hiPSC-CMs differentiation. Sci Rep 9(1):19389. https://doi.org/10.1038/s41598-019-55620-x

Article  CAS  PubMed  PubMed Central  Google Scholar 

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