Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979;76(9):4350–4.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Eaton SL, Roche SL, Llavero Hurtado M, Oldknow KJ, Farquharson C, Gillingwater TH, et al. Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting. PLoS ONE. 2013;8(8):e72457.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ni D, Xu P, Gallagher S. Immunoblotting and immunodetection. Curr Protoc Cell Biol. 2017;74(6 2 1–6 2):37.

Google Scholar 

Taylor SC, Berkelman T, Yadav G, Hammond M. A defined methodology for reliable quantification of Western blot data. Mol Biotechnol. 2013;55(3):217–26.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen GF, Zhang CY, Wang YY, Chen W. Application of reverse dot blot hybridization to simultaneous detection and identification of harmful algae. Environ Sci Pollut Res Int. 2015;22(14):10516–28.

Article  CAS  PubMed  Google Scholar 

Reyes-Serratos E, Marcet-Palacios M, Rosenfield D, Ritz T, Befus AD. A method to study protein biomarkers in saliva using an automated capillary nano-immunoassay platform (Wes). J Immunol Methods. 2020;479:112749.

Article  CAS  PubMed  Google Scholar 

Pillai-Kastoori L, Schutz-Geschwender AR, Harford JA. A systematic approach to quantitative Western blot analysis. Anal Biochem. 2020;593:113608.

Article  CAS  PubMed  Google Scholar 

Uyen Nguyen NS, Annegret B, Peter A, Fung. The simple Western™: a gel-free, blot-free, hands-free Western blotting reinvention. Nat Methods. 2011;8:v–vi.

Article  Google Scholar 

Taylor SC, Rosselli-Murai LK, Crobeddu B, Plante I. A critical path to producing high quality, reproducible data from quantitative Western blot experiments. Sci Rep. 2022;12(1):17599.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang J, Valdez A, Chen Y. Evaluation of automated Wes system as an analytical and characterization tool to support monoclonal antibody drug product development. J Pharm Biomed Anal. 2017;139:263–8.

Article  CAS  PubMed  Google Scholar 

Chen JQ, Heldman MR, Herrmann MA, Kedei N, Woo W, Blumberg PM, et al. Absolute quantitation of endogenous proteins with precision and accuracy using a capillary western system. Anal Biochem. 2013;442(1):97–103.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Beekman C, Janson AA, Baghat A, van Deutekom JC, Datson NA. Use of capillary western immunoassay (Wes) for quantification of dystrophin levels in skeletal muscle of healthy controls and individuals with Becker and Duchenne muscular dystrophy. PLoS ONE. 2018;13(4):e0195850.

Article  PubMed  PubMed Central  Google Scholar 

Tie L, Xiao H, Wu DL, Yang Y, Wang P. A brief guide to good practices in pharmacological experiments: Western blotting. Acta Pharmacol Sin. 2021;42(7):1015–7.

Article  CAS  PubMed  Google Scholar 

Sabine Lennarz K, Lili AG. Yu, Agnieszka Kieloch and Marie-Anne Valentin. Fit-for-purpose method validation of clinical biomarker assays to show proof of biology with automated western blot technologies. AAPS PharmSci-360; San Antonio, Texas 2019.

Eric Chen SM. Arvind Kinhikar and Marie-Anne Valentin New immunoassay platforms: a practical guide to method validation AAPS Bational Biotechnology Conference 2018.

Meftahi GH, Bahari Z, Zarei Mahmoudabadi A, Iman M, Jangravi Z. Applications of Western blot technique: from bench to bedside. Biochem Mol Biol Educ. 2021;49(4):509–17.

Article  CAS  PubMed  Google Scholar 

Harris VM. Protein detection by simple western analysis. Methods Mol Biol. 2015;1312:465–8.

Article  PubMed  Google Scholar 

Gentalen ET, Proctor JM. Using the Peggy simple western system for fine needle aspirate analysis. Methods Mol Biol. 2015;1219:139–55.

Article  CAS  PubMed  Google Scholar 

Rizner TL, Adamski J. It is high time to discontinue use of misidentified and contaminated cells: guidelines for description and authentication of cell lines. J Steroid Biochem Mol Biol. 2018;182:1–3.

Article  CAS  PubMed  Google Scholar 

Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, et al. A proposal for validation of antibodies. Nat Methods. 2016;13(10):823–7.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Edfors F, Hober A, Linderback K, Maddalo G, Azimi A, Sivertsson A, et al. Enhanced validation of antibodies for research applications. Nat Commun. 2018;9(1):4130.

Article  PubMed  PubMed Central  Google Scholar 

Kedei N, Telek A, Michalowski AM, Kraft MB, Li W, Poudel YB, et al. Comparison of transcriptional response to phorbol ester, bryostatin 1, and bryostatin analogs in LNCaP and U937 cancer cell lines provides insight into their differential mechanism of action. Biochem Pharmacol. 2013;85(3):313–24.

Article  CAS  PubMed  Google Scholar 

Yang CR, Raghuram V, Emamian M, Sandoval PC, Knepper MA. Deep proteomic profiling of vasopressin-sensitive collecting duct cells. II. Bioinformatic analysis of vasopressin signaling. Am J Physiol Cell Physiol. 2015;309(12):C799–812.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lange N, Unger FT, Schoppler M, Pursche K, Juhl H, David KA. Identification and validation of a potential marker of tissue quality using gene expression analysis of human colorectal tissue. PLoS ONE. 2015;10(7):e0133987.

Article  PubMed  PubMed Central  Google Scholar 

Buccitelli C, Selbach M. mRNAs, proteins and the emerging principles of gene expression control. Nat Rev Genet. 2020;21(10):630–44.

Article  CAS  PubMed  Google Scholar 

Vincent A. Antibodies associated with paraneoplastic neurological disorders. Neurol Sci. 2005;26(Suppl 1):S3–4.

Article  PubMed  Google Scholar 

Landry JP, Ke Y, Yu GL, Zhu XD. Measuring affinity constants of 1450 monoclonal antibodies to peptide targets with a microarray-based label-free assay platform. J Immunol Methods. 2015;417:86–96.

Article  CAS  PubMed  Google Scholar 

Mohammed H, Taylor C, Brown GD, Papachristou EK, Carroll JS, D’Santos CS. Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes. Nat Protoc. 2016;11(2):316–26.

Article  CAS  PubMed  Google Scholar 

Lavatelli F, Valentini V, Palladini G, Verga L, Russo P, Foli A, et al. Mass spectrometry-based proteomics as a diagnostic tool when immunoelectron microscopy fails in typing amyloid deposits. Amyloid. 2011;18(Suppl 1):64–6.

Article  PubMed  Google Scholar 

Zhang R, Yang D, Zhou C, Cheng K, Liu Z, Chen L, et al. beta-actin as a loading control for plasma-based Western blot analysis of major depressive disorder patients. Anal Biochem. 2012;427(2):116–20.

Article  CAS  PubMed  Google Scholar 

Li R, Shen Y. An old method facing a new challenge: re-visiting housekeeping proteins as internal reference control for neuroscience research. Life Sci. 2013;92(13):747–51.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Faden F, Eschen-Lippold L, Dissmeyer N. Normalized quantitative western blotting based on standardized fluorescent labeling. Methods Mol Biol. 2016;1450:247–58.

Article  CAS  PubMed