Hughes K. Comparative mammary gland postnatal development and tumourigenesis in the sheep, cow, cat and rabbit: exploring the menagerie. Semin Cell Dev Biol. 2021;114:186–95.

Article  PubMed  Google Scholar 

Nagy D, Gillis CMC, Davies K, Fowden AL, Rees P, Wills JW, Hughes K. Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment. Commun Biol. 2021;4(1):993.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Vang AL, Bresolin T, Frizzarini WS, Menezes GL, Cunha T, Rosa GJM, Hernandez LL, Dorea JRR. Longitudinal analysis of bovine mammary gland development. J Mammary Gland Biol Neoplasia. 2023;28(1):11.

Article  PubMed  PubMed Central  Google Scholar 

Hughes K, Watson CJ. The spectrum of STAT functions in mammary gland development. JAKSTAT. 2012;1(3):151–8.

PubMed  PubMed Central  Google Scholar 

Chapman RS, Lourenco PC, Tonner E, Flint DJ, Selbert S, Takeda K, Akira S, Clarke AR, Watson CJ. Suppression of epithelial apoptosis and delayed mammary gland involution in mice with a conditional knockout of Stat3. Genes Dev. 1999;13(19):2604–16.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kritikou EA, Sharkey A, Abell K, Came PJ, Anderson E, Clarkson RW, Watson CJ. A dual, non-redundant, role for LIF as a regulator of development and STAT3-mediated cell death in mammary gland. Development. 2003;130(15):3459–68.

Article  CAS  PubMed  Google Scholar 

Singh K, Vetharaniam I, Dobson JM, Prewitz M, Oden K, Murney R, Swanson KM, McDonald R, Henderson HV, Stelwagen K. Cell survival signaling in the bovine mammary gland during the transition from lactation to involution. J Dairy Sci. 2016;99(9):7523–43.

Article  CAS  PubMed  Google Scholar 

Singh K, Phyn CVC, Reinsch M, Dobson JM, Oden K, Davis SR, Stelwagen K, Henderson HV, Molenaar AJ. Temporal and spatial heterogeneity in milk and immune-related gene expression during mammary gland involution in dairy cows. J Dairy Sci. 2017;100(9):7669–85.

Article  CAS  PubMed  Google Scholar 

Kreuzaler PA, Staniszewska AD, Li W, Omidvar N, Kedjouar B, Turkson J, Poli V, Flavell RA, Clarkson RW, Watson CJ. Stat3 controls lysosomal-mediated cell death in vivo. Nat Cell Biol. 2011;13(3):303–9.

Article  CAS  PubMed  Google Scholar 

Sargeant TJ, Lloyd-Lewis B, Resemann HK, Ramos-Montoya A, Skepper J, Watson CJ. Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization. Nat Cell Biol. 2014;16(11):1057–68.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hughes K, Wickenden JA, Allen JE, Watson CJ. Conditional deletion of Stat3 in mammary epithelium impairs the acute phase response and modulates immune cell numbers during post-lactational regression. J Pathol. 2012;227(1):106–17.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sordillo LM, Streicher KL. Mammary gland immunity and mastitis susceptibility. J Mammary Gland Biol Neoplasia. 2002;7(2):135–46.

Article  PubMed  Google Scholar 

McCracken JY, Molenaar AJ, Wilkins RJ, Grigor MR. Spatial and temporal expression of transferrin gene in the rat mammary gland. J Dairy Sci. 1994;77(7):1828–34.

Article  CAS  PubMed  Google Scholar 

Quaglino A, Salierno M, Pellegrotti J, Rubinstein N, Kordon EC. Mechanical strain induces involution-associated events in mammary epithelial cells. BMC Cell Biol. 2009;10:55.

Article  PubMed  PubMed Central  Google Scholar 

Ohsawa K, Imai Y, Kanazawa H, Sasaki Y, Kohsaka S. Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia. J Cell Sci. 2000;113(Pt 17):3073–84.

Article  CAS  PubMed  Google Scholar 

Gordon S, Martinez FO. Alternative activation of macrophages: mechanism and functions. Immunity. 2010;32(5):593–604.

Article  CAS  PubMed  Google Scholar 

Clarkson RW, Wayland MT, Lee J, Freeman T, Watson CJ. Gene expression profiling of mammary gland development reveals putative roles for death receptors and immune mediators in post-lactational regression. Breast Cancer Res. 2004;6(2):R92–109.

Article  CAS  PubMed  Google Scholar 

Stein T, Morris JS, Davies CR, Weber-Hall SJ, Duffy MA, Heath VJ, Bell AK, Ferrier RK, Sandilands GP, Gusterson BA. Involution of the mouse mammary gland is associated with an immune cascade and an acute-phase response, involving LBP, CD14 and STAT3. Breast Cancer Res. 2004;6(2):R75–91.

Article  CAS  PubMed  Google Scholar 

Molenaar AJ, Davis SR, Wilkins RJ. Expression of alpha-lactalbumin, alpha-S1-casein, and lactoferrin genes is heterogeneous in sheep and cattle mammary tissue. J Histochem Cytochem. 1992;40(5):611–8.

Article  CAS  PubMed  Google Scholar 

Oliver SP, Sordillo LM. Udder health in the periparturient period. J Dairy Sci. 1988;71(9):2584–606.

Article  CAS  PubMed  Google Scholar 

Molenaar AJ, Wheeler TT, Grigor MR. Nuclear localisation of the transcription factor Stat5b is associated with ovine milk protein gene expression during lactation but not during late pregnancy or forced weaning. Histochem J. 2000;32(5):265–74.

Article  CAS  PubMed  Google Scholar 

Summers HD, Wills JW, Rees P. Spatial statistics is a comprehensive tool for quantifying cell neighbor relationships and biological processes via tissue image analysis. Cell Rep Methods. 2022;2(11):100348.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Twigger AJ, Khaled WT. Mammary gland development from a single cell ‘omics view. Semin Cell Dev Biol. 2021;114:171–85.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hughes K. Studying Mammary Physiology and Pathology in domestic species benefits both humans and animals. J Mammary Gland Biol Neoplasia. 2023;28(1):18.

Article  PubMed  PubMed Central  Google Scholar 

Wills JW, Robertson J, Summers HD, Miniter M, Barnes C, Hewitt RE, Keita AV, Soderholm JD, Rees P, Powell JJ. Image-Based Cell Profiling Enables Quant Tissue Microscopy Gastroenterol Cytometry A. 2020;97(12):1222–37.

CAS  Google Scholar 

Cromley RG, Hanink DM, Bentley GC. Geographically weighted colocation quotients: specification and application. Prof Geogr. 2014;66(1):138–48.

Article  Google Scholar 

Wills JW, Robertson J, Tourlomousis P, Gillis CMC, Barnes CM, Miniter M, Hewitt RE, Bryant CE, Summers HD, Powell JJ, Rees P. Label-free cell segmentation of diverse lymphoid tissues in 2D and 3D Cell Rep Methods, 2023. 3(2): 100398.