1 Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
2 Present address: Department of Biotechnology, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidoubata-Nishi, Shimoshinjo-Nakano, Akita, Akita 010-0195, Japan.
3 Present address: Engineering Biology Research Center, Kobe University, 7-1-48, Minatojima Minami-machi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
4 Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
# The first two authors contributed equally to the manuscript.

Keywords: metallothionein, nitrosative stress, yeast, stress tolerance.

Abbreviations:

MT – Metallothioein,

NO – nitric oxide,

RNS – reactive nitrogen species,

fHb – flavohemoglobin,

GCH2 – GTP cyclohydrolase Ⅱ,

GSNO – S-nitrosoglutathione,

GSNOR – GSNO reductase,

Trx – thioredoxin,

Trr – thioredoxin reductase,

WT – wild-type,

SD – synthetic dextrose,

DAF-FM DA – 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate,

NOC-5 – 3-[2-hydroxy-1-(1-methylethyl)-2-nitrosohydrazino]-1-propanamine,

Grx – glutaredoxin,

Grr – glutaredoxin.
Received originally: 06/04/2023 Received in revised form: 26/06/2023
Accepted: 27/06/2023 Published: 10/07/2023

Correspondence:
Hiroshi Takagi, Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; hiro@bs.naist.jp

Conflict of interest statement:

No potential conflict of interest was reported by the authors.
Please cite this article as: Yuki Yoshikawa, Ryo Nasuno, Naoki Takaya, Hiroshi Takagi (2023). Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae. Microbial Cell10(8):