Testicular microlithiasis and male fertility: a dual perspective integrating imaging and physiological insights

Anvari Aria S, et al. Testicular microlithiasis on scrotal ultrasound in 4850 young men from the general population: associations with semen quality. Andrology. 2020;8(6):1736–43.

Article  PubMed  CAS  Google Scholar 

Zhu QF, et al. [Influence of testicular microlithiasis on male fertility: an update]. Zhonghua Nan Ke Xue. 2021;27(12):1119–23.

PubMed  Google Scholar 

Barbonetti A, et al. Testicular cancer in infertile men with and without testicular microlithiasis: A systematic review and Meta-Analysis of Case-Control studies. Front Endocrinol (Lausanne). 2019;10:164.

Article  PubMed  Google Scholar 

Frandsen RH, et al. Testicular biopsies in men with testicular microlithiasis and additional risk factors for cancer: A case series. Andrology. 2024;12(8):1764–70.

Article  PubMed  Google Scholar 

Hiramatsu I, et al. Prevalence of testicular microlithiasis in healthy newlywed men trying for first-time pregnancy. Int J Urol. 2020;27(11):990–5.

Article  PubMed  CAS  Google Scholar 

Rassam Y, et al. Testicular microlithiasis is associated with impaired spermatogenesis in patients with unexplained infertility. Urol Int. 2020;104(7–8):610–6.

Article  PubMed  CAS  Google Scholar 

Nakamura M, et al. Prevalence and risk factors of testicular microlithiasis in patients with hypospadias: a retrospective study. BMC Pediatr. 2018;18(1):179.

Article  PubMed  PubMed Central  Google Scholar 

Taylor JF, Schnabel RD, Sutovsky P. Identification of genomic variants causing sperm abnormalities and reduced male fertility. Anim Reprod Sci. 2018;194:57–62.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Spaziani M, et al. The role of scrotal ultrasonography from infancy to puberty. Andrology. 2021;9(5):1306–21.

Article  PubMed  PubMed Central  Google Scholar 

Yoshimura S, et al. Incidence of testicular microlithiasis after Orchidopexy for cryptorchidism and its risk factors: A retrospective cohort study. J Pediatr Urol. 2022;18(3):377. .e1-377.e5.

Article  Google Scholar 

Maiolino G et al. Male infertility and the risk of developing testicular cancer: A critical contemporary literature review. Med (Kaunas). 2023;59(7):1305.

Liu X, et al. Improved ultrasound imaging performance with complex cumulant analysis. IEEE Trans Biomed Eng. 2022;69(3):1281–9.

Article  PubMed  Google Scholar 

Prasun DAS. Application of scrotal ultrasound imaging in clinicopathologically suspected cases of male infertility: A prospective study in a peripheral medical college in West Bengal. Asian J Pharm Clin Res. 2024;17(8):78–81.

Article  Google Scholar 

Oluseyi K-YH, Yunusa T. Sonography of scrotal abnormality among subfertile and fertile males in abuja, Nigeria. Sub-Saharan Afr J Med. 2019;6(2):101–105.

Pourjabali M, Noushyar M, Ghahramanpour A, Mansouri F. A testicular mass with extensive calcification and ossification: A case report. SAGE Open Medical Case Reports. 2024;12(3):1–5.

Yu CJ, et al. Incidence characteristics of testicular microlithiasis and its association with risk of primary testicular tumors in children: a systematic review and meta-analysis. World J Pediatr. 2020;16(6):585–97.

Article  PubMed  Google Scholar 

t Hoen LA, et al. The prognostic value of testicular microlithiasis as an incidental finding for the risk of testicular malignancy in children and the adult population: A systematic review. On behalf of the EAU pediatric urology guidelines panel. J Pediatr Urol. 2021;17(6):815–31.

Article  PubMed  Google Scholar 

D’Andrea S, et al. Clinical and seminal parameters associated with testicular microlithiasis and its severity in males from infertile couples. Hum Reprod. 2021;36(4):891–8.

Article  PubMed  Google Scholar 

Xu Z, et al. The diagnostic value of ultrasound in pediatric testicular torsion with preserved flow. Front Pediatr. 2022;10:1001958.

Article  PubMed  PubMed Central  Google Scholar 

Samir H, Radwan F, Watanabe G. Advances in applications of color doppler ultrasonography in the andrological assessment of domestic animals: A review. Theriogenology. 2021;161:252–61.

Article  PubMed  Google Scholar 

Tenuta M, et al. Testicular ultrasonographic features predict future risk for bilateral testicular germ cell tumour: A long-term single centre follow-up study. Andrology. 2025;13(3):587–99.

Article  PubMed  CAS  Google Scholar 

Tsili AC, et al. Ultrasonography of the scrotum: revisiting a classic technique. Eur J Radiol. 2021;145:110000.

Article  PubMed  Google Scholar 

Aslan S, et al. The role of ARFI elastography to evaluate microstructrual changes of patients with testicular microlithiasis. Acta Radiol. 2018;59(12):1517–22.

Article  PubMed  Google Scholar 

Prado-Costa R, et al. Ultrasound elastography: compression elastography and shear-wave elastography in the assessment of tendon injury. Insights Imaging. 2018;9(5):791–814.

Article  PubMed  PubMed Central  Google Scholar 

de Jong BWD, De Gouveia Brazao CA, Stoop H, Wolffenbuttel KP, Oosterhuis JW, Puppels GJ, Kok DJ. Raman Spectroscopic Analysis Identifies Testicular Microlithiasis as Intratubular Hydroxyapatite. J Urol. 2004. 171(1), 92–96.

Tang QL, Dai YT. [Advances in the studies of testicular microlithiasis]. Zhonghua Nan Ke Xue. 2017;23(8):734–8.

PubMed  Google Scholar 

Boisen IM et al. Changes in local mineral homeostasis facilitate the formation of benign and malignant testicular microcalcifications. Elife. 2025;13:RP95545.

Du M, et al. Testicular fat deposition attenuates reproductive performance via decreased follicle-stimulating hormone level and sperm meiosis and testosterone synthesis in mouse. Anim Biosci. 2024;37(1):50–60.

Article  PubMed  CAS  Google Scholar 

Dantsev IS, et al. Genes associated with testicular germ cell tumors and testicular dysgenesis in patients with testicular microlithiasis. Asian J Androl. 2018;20(6):593–9.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Zheng Q, et al. Loss of Cyp11c1 causes delayed spermatogenesis due to the absence of 11-ketotestosterone. J Endocrinol. 2020;244(3):487–99.

Article  PubMed  CAS  Google Scholar 

Li J et al. X-linked RBBP7 mutation causes maturation arrest and testicular tumors. J Clin Invest, 2023;133(20):e171541.

Sreenivas A, et al. A case of primary extragonadal germ cell malignancy with testicular microlithiasis: its association and significance. Cureus. 2025;17(3):e80018.

PubMed  PubMed Central  Google Scholar 

Sheyin J, Chom ND. Testicular microlithiasis seen in primary infertility. West Afr J Radiol. 2021;28(1):21.

Zhang J, et al. 337: six characters of seminiferous tubules appearances during Micro-Tese related with different sperm retrieval rate. Fertil Reprod. 2023;5(4):568–9.

Article  Google Scholar 

Mahdy B, et al. Microsurgical testicular sperm extraction: predictive factors and outcomes for men with nonobstructive azoospermia. Andrologia. 2024;2024(1):6380023.

Article  Google Scholar 

Jensen CFS, et al. Microdissection testicular sperm extraction versus multiple Needle-pass percutaneous testicular sperm aspiration in men with nonobstructive azoospermia: A randomized clinical trial. Eur Urol. 2022;82(4):377–84.

Article  PubMed  Google Scholar 

Colpi GM, Caroppo E. Performing microdissection testicular sperm extraction: Surgical pearls from a high-volume infertility center. J Clin Med. 2021;10(19):4296.

Salama N. Letter to editor: we need standardized guidelines for laboratory tissue processing after microsurgical testicular sperm extraction. Reprod Sci. 2021;28(8):2071–5.

Article  PubMed  Google Scholar 

Amer M, Fakhry E. Fresh vs frozen testicular sperm for assisted reproductive technology in patients with non-obstructive azoospermia: A systematic review. Arab J Urol. 2021;19(3):247–54.

Article  PubMed  PubMed Central  Google Scholar 

Jahangiri AR, et al. Microfluidics: the future of sperm selection in assisted reproduction. Andrology. 2024;12(6):1236–52.

Article  PubMed  CAS  Google Scholar 

Mulla N. Mediastinal germ cell tumor with testicular microlithiasis. Cureus. 2021;13(1):e12761.

PubMed  PubMed Central  Google Scholar 

Yao M, Satchi M. Testicular microlithiasis: a cause for concern? Trends Urol Men’s Health. 2023;14(5):19–23.

Article  Google Scholar 

Chaka A, et al. Pediatric testicular microlithiasis through four clinical case studies: review of the literature and proposal of clinical guidelines. Afr J Urol. 2021;27(1):122.

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