Hamostaseologie
DOI: 10.1055/a-2462-6667
1
School of Medicine, Research Center for High Altitude Medicine, Qinghai University,
Xining, China
2
Geriatric department, Qinghai University Affiliated Hospital, Xining, China
3
Key Laboratory of Application and Foundation for High Altitude Medicine Research,
Qinghai Province, Xining, China
,
1
School of Medicine, Research Center for High Altitude Medicine, Qinghai University,
Xining, China
3
Key Laboratory of Application and Foundation for High Altitude Medicine Research,
Qinghai Province, Xining, China
,
Yi Ye
1
School of Medicine, Research Center for High Altitude Medicine, Qinghai University,
Xining, China
3
Key Laboratory of Application and Foundation for High Altitude Medicine Research,
Qinghai Province, Xining, China
,
Yi Wen
4
Department of General Surgery & Pancreatic Injury and Repair Key Laboratory of Sichuan
Province, The General Hospital of Western Theater Command (Chengdu Military General
Hospital), Chengdu, China
,
Tana Wuren
1
School of Medicine, Research Center for High Altitude Medicine, Qinghai University,
Xining, China
3
Key Laboratory of Application and Foundation for High Altitude Medicine Research,
Qinghai Province, Xining, China
› Author AffiliationsFunding This work was supported by the Sichuan Science and Technology Program (2023YFQ0068);
the General Hospital of Western Theater Command (2021-XZYG-C30).
› Further InformationAlso available at
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Abstract
Chronic high-altitude hypoxia is associated with reduced platelet count, but it is
unclear whether the decrease in platelet count is due to impaired production or increased
clearance. This study examines how hypoxia affects platelet production and apoptosis
and elucidates the impact of glycoprotein Ibα–von Willebrand factor interaction on
platelets in rats using a hypobaric hypoxia chamber. The results showed that the number
of megakaryocytes increased under hypoxia; however, the levels of differentiation
and polyploidy decreased, while those of apoptosis increased. Platelet production
did not reduce according to the reticulated platelet percentage, while platelet apoptosis
enhanced; these results suggest that increased platelet clearance was the main reason
behind platelet reduction. Our previous microarray results indicated that glycoprotein
Ibα (GPIbα) expression increased under hypoxia, which was a protein involved in platelet
clearance; therefore, we examined the interaction of platelet GPIbα with the von Willebrand
factor (vWF) both in vivo and in vitro to explore the effect of this process on platelets
and whether it is related to platelet apoptosis. Under hypoxia, the stronger interaction
between GPIbα and vWF promoted platelet apoptosis; inhibiting this interaction reduced
platelet apoptosis and increased platelet counts. Platelet reduction is associated
with apoptosis induced by the interaction between GPIbα and vWF.
Keywords
apoptosis -
glycoprotein Ib alpha -
hypoxia -
platelet -
von Willebrand factor
Data Availability
The datasets generated for this study can be found in the figshare database (https://doi.org/10.6084/m9.figshare.23993115).
Authors' Contributions
T.W. and Z.W. conceived and designed the research; Z.W. and D.G. performed the experiments
and analysed the data; Z.W. and Y.Y. prepared the figures; Y.Y. assisted in the experiment;
Z.W. and D.G. drafted and revised the manuscript; and Y.W. and T.W. approved the final
version of the manuscript. Z.W. and D.G. contributed equally to this work and should
be considered co-first authors.
*These authors contributed equally to this work and should be considered co-first authors.
Publication History
Received: 13 June 2024
Accepted: 05 November 2024
Article published online:
28 March 2025
© 2025. Thieme. All rights reserved.
Georg Thieme Verlag KG
Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany
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