The vital role of TRPM2 in breast cancer cell proliferation and its difference in expression between non-Hispanic white and non-Hispanic black females

The vital role of TRPM2 in breast cancer cell proliferation and its difference in expression between non-Hispanic white and non-Hispanic black females WCRJ 2025; 12 : e2870
DOI: 10.32113/wcrj_20255_2870

Topic: Breast cancer Category: Original article

Huang L. P. Immunology & Transplant Science Center, Houston Methodist Research Institute, Houston, TX, USA , Lu Y. Center for Aging, School of Medicine, Tulane University, New Orleans, LA, USA , Li M. Department of Physiology, Tulane University Health Sciences Center, New Orleans, LA, USA
mli@tulane.edu Abstract Objective: To study the racial disparity of transient receptor potential melastatin-related channel subtype 2 (TRPM2) expression in non-Hispanic black (NHB) women and non-Hispanic white women (NHW) breast cancer tissues. Specific roles of TRPM2 in the proliferation and apoptosis of the human mitogenic breast cancer cells (MCF-7 ER+) are also investigated.

Materials and Methods: TRPM2 expression analysis was performed using the normalized mRNA expression data downloaded from Broad GDAC Firehose. The functions of TRPM2 in normal and cancer cells are revealed using patch-clamp recording and intracellular calcium imaging techniques.

Results: TRPM2 is expressed at different levels in breast ductal and lobular neoplasm disease between NHB and NHW. Selective knock-down of TRPM2 mRNA with small interfering RNA (siRNA/TRPM2) caused substantial inhibition of cell proliferation of MCF-7(ER+) cells but not in the control human mammary epithelial (HME) cells. The siRNA/TRPM2 also induced cellular apoptosis in MCF-7(ER+) cells but not HME cells. Immunofluorescent staining shows that TRPM2 abnormally localizes in the MCF-7(ER+) nuclei but not HME cells.

Conclusions: These results suggest that TRPM2 is essential in promoting MCF-7(ER+) cell proliferation and may be a potential target for breast cancer treatment.

To cite this article Huang L. P. Immunology & Transplant Science Center, Houston Methodist Research Institute, Houston, TX, USA , Lu Y. Center for Aging, School of Medicine, Tulane University, New Orleans, LA, USA , Li M. Department of Physiology, Tulane University Health Sciences Center, New Orleans, LA, USA
mli@tulane.edu The vital role of TRPM2 in breast cancer cell proliferation and its difference in expression between non-Hispanic white and non-Hispanic black females

WCRJ 2025; 12 : e2870
DOI: 10.32113/wcrj_20255_2870

Publication History

Submission date: 29 Jul 2024

Revised on: 02 Sep 2024

Accepted on: 23 Dec 2024

Published online: 07 May 2025