The increasing need, for point-of-care (POC) testing has prompted a rise, in the popularity of affordable biosensors that are eco-friendly, especially paper based electrochemical sensors. The research introduces biodegradable paper-based enzymatic biosensor for detecting glucose levels in intricate biological samples such, as cell lysates. This biosensor uses Prussian Blue (PB) as a mediator and glucose oxidase to detect glucose with excellent accuracy using direct electrochemical signals. Screen printing using Whatman filter paper produced a better biosensor than other substrates. The PB concentration of 12.5 mmol L-1 was found to be optimal and resulted in an operating potential of -0.1 V which helped decrease interference, from other active substances and improved its selectivity. Calibration was found to be linear up to concentration 2 mmol L-1 with detection limit of 40 mol L-1 and limit of quantification 120 mol L-1 . Moreover, experiments performed on cell lysates obtained from peripheral blood mononuclear cells (PBMCs) suggest the possible application of biosensors to measure glucose levels in both in vitro stimulated and unstimulated cells. This feature underscores its promise, for use, in monitoring metabolism and conducting diagnostic applications. The paper-based biosensor is an alternative to the current platform for the development and of eco-friendly portable, glucose sensitive biosensor for monitoring glucose at point of care. Its flexibility and efficiency make it a strong candidate for use in the field of POC diagnostics especially in the areas of limited resources and in conditions where there is a problem with glucose dysregulation including diabetes and other related metabolic disorders.

This article is Open Access

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