Optimized sperm selection: a highly efficient device for the isolation of progressive motile sperm with low DNA fragmentation index

Sakkas D, Ramalingam M, Garrido N, Barratt CL. Sperm selection in natural conception: what can we learn from Mother Nature to improve assisted reproduction outcomes? Hum Reprod Update. 2015;21(6):711–26.https://doi.org/10.1093/humupd/dmv042.

Zorn B, Golob B, Ihan A, Kopitar A, Kolbezen M. Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential. J Assist Reprod Genet. 2012;29(4):357–64. https://doi.org/10.1007/s10815-012-9718-x.

Article  PubMed  PubMed Central  Google Scholar 

Shen HM, Dai J, Chia SE, Lim A, Ong CN. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Hum Reprod. 2002;17(5):1266–73. https://doi.org/10.1093/humrep/17.5.1266.

Article  PubMed  Google Scholar 

Sedó CA, Bilinski M, Lorenzi D, Uriondo H, Noblía F, Longobucco V, et al. Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects. JBRA Assist Reprod. 2017;21(4):343–50.

PubMed Central  Google Scholar 

Wang H, Li H, Zhu J, Xu J, Jiang Y, Chen W, et al. The effect of sperm DNA fragmentation on in vitro fertilization outcomes for women with polycystic ovary syndrome. Front Endocrinol (Lausanne). 2022;13:822786. https://doi.org/10.3389/fendo.2022.822786.

Article  PubMed  Google Scholar 

Lourenço ML, Moura GA, Rocha YM, Rodrigues JPV, Monteiro PB. Impact of sperm DNA fragmentation on the clinical outcome of assisted reproduction techniques: a systematic review of the last five years. JBRA Assist Reprod. 2023;27(2):282–91. https://doi.org/10.5935/1518-0557.20220057.

Article  PubMed  PubMed Central  Google Scholar 

Antonouli S, Papatheodorou A, Panagiotidis Y, Petousis S, Prapas N, Nottola SA, et al. The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes. Arch Gynecol Obstet. 2019;300(1):207–15. https://doi.org/10.1007/s00404-019-05133-9.

Article  CAS  PubMed  Google Scholar 

Liu K, Mao X, Pan F, Chen Y, An R. Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART. Sci Rep. 2023;13(1):2717. https://doi.org/10.1038/s41598-023-28765-z.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Said T, Agarwal A, Grunewald S, Rasch M, Baumann T, Kriegel C, et al. Selection of nonapoptotic spermatozoa as a new tool for enhancing assisted reproduction outcomes: an in vitro model. Biol Reprod. 2006;74:530–7. https://doi.org/10.1095/biolreprod.105.046607.

Article  CAS  PubMed  Google Scholar 

Said TM, Land JA. Effects of advanced selection methods on sperm quality and ART outcome: a systematic review. Hum Reprod Update. 2011;17(6):719–33.

Article  PubMed  Google Scholar 

Gil M, Sar-Shalom V, Sivira YM, Carreras R, Checa MA. Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis. J Assist Reprod Genet. 2013;30(4):479–85. https://doi.org/10.1007/s10815-013-9962-8.

Article  PubMed  PubMed Central  Google Scholar 

Asghar W, Velasco V, Kingsley JL, Shoukat MS, Shafiee H, Anchan RM, et al. Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Health Mater. 2014;3(10):1671–9. https://doi.org/10.1002/adhm.201400058.

Article  CAS  Google Scholar 

Gode F, Gürbüz AS, Tamer B, Pala I, Isik AZ. The effects of microfluidic sperm sorting, density gradient and swim-up methods on semen oxidation reduction potential. Urol J. 2020;17(4):397–401. https://doi.org/10.22037/uj.v0i0.5639.

Article  PubMed  Google Scholar 

Yildiz K, Yuksel S. Use of microfluidic sperm extraction chips as an alternative method in patients with recurrent in vitro fertilisation failure. J Assist Reprod Genet. 2019;36(7):1423–9. https://doi.org/10.1007/s10815-019-01480-3.

Article  PubMed  PubMed Central  Google Scholar 

Yalcinkaya Kalyan E, Can Celik S, Okan O, Akdeniz G, Karabulut S, Caliskan E. Does a microfluidic chip for sperm sorting have a positive add-on effect on laboratory and clinical outcomes of intracytoplasmic sperm injection cycles? A sibling oocyte study. Andrologia. 2019;51(10): e13403. https://doi.org/10.1111/and.13403.

Article  PubMed  Google Scholar 

Gode F, Bodur T, Gunturkun F, Gurbuz AS, Tamer B, Pala I, et al. Comparison of microfluid sperm sorting chip and density gradient methods for use in intrauterine insemination cycles. Fertil Steril. 2019;112(5):842-848.e1. https://doi.org/10.1016/j.fertnstert.2019.06.037.

Article  CAS  PubMed  Google Scholar 

Parella A Keating, D, Cheung S, Xie P, Stewart GD, Rosenwaks Z, et al. A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure. J Assist Reprod Genet. 2019;36(10):2057–2066. https://doi.org/10.1007/s10815-019-01543-5.

World Health Organization. WHO laboratory manual for the examination and processing of human semen. 6th ed. World Health Organization, Geneva; 2021. Available at: https://www.who.int/publications/i/item/9789240030787.

Fleiss JL. Design and analysis of clinical experiments. New York: Willey; 1986.

Google Scholar 

Quinn MM, Jalalian L, Ribeiro S, Ona K, Demirci U, Cedars MI, et al. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod. 2018;33(8):1388–93. https://doi.org/10.1093/humrep/dey239.

Article  CAS  PubMed  Google Scholar 

Quinn MM, Ribeiro S, Juarez-Hernandez F, Simbulan RK, Jalalian L, Cedars MI, et al. Microfluidic preparation of spermatozoa for ICSI produces similar embryo quality to density-gradient centrifugation: a pragmatic, randomized controlled trial. Hum Reprod. 2022;37(7):1406–13. https://doi.org/10.1093/humrep/deac099.

Article  CAS  PubMed  Google Scholar 

Martínez MG, Sánchez-Martín P, Dorado-Silva M, Fernández JL, Girones E, Johnston SD, et al. Magnetic-activated cell sorting is not completely effective at reducing sperm DNA fragmentation. J Assist Reprod Genet. 2018;35(12):2215–21. https://doi.org/10.1007/s10815-018-1319-x.

Article  PubMed  PubMed Central  Google Scholar 

Novin SM, Mehdizadeh A, Artimani T, Bakhtiari M, Mehdizadeh M, Aflatoonian R, et al. MACS-DGC sperm preparation method resulted in high-quality sperm, top-quality embryo, and higher blastocyst rate in male factor infertile couples with high DNA fragmented sperm. Hum Fertil (Camb). 2023;1–9. https://doi.org/10.1080/14647273.2023.2236297. Online ahead of print.

Berteli TS, Da Broi MG, Martins WP, Ferriani RA, Navarro PA. Magnetic-activated cell sorting before density gradient centrifugation improves recovery of high-quality spermatozoa. Andrology. 2017;5:776–82. https://doi.org/10.1111/andr.12372.

Article  CAS  PubMed  Google Scholar 

Hanson BM, Aston KI, Jenkins TG, Carrell DT, Hotaling JM. The impact of ejaculatory abstinence on semen analysis parameters: a systematic review. J Assist Reprod Genet. 2018;35(2):213–20. https://doi.org/10.1007/s10815-017-1086-0.

Article  PubMed  Google Scholar 

Barbagallo F, Cannarella R, Crafa A, Manna C, La Vignera S, Condorelli RA, et al. The impact of a very short abstinence period on conventional sperm parameters and sperm DNA fragmentation: a systematic review and meta-analysis. J Clin Med. 2022;11(24):7303. https://doi.org/10.3390/jcm11247303.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Aitken RJ, Clarkson JS. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J Androl. 1988;9:367–76. https://doi.org/10.1002/j.1939-4640.1988.tb01067.

Article  CAS  PubMed  Google Scholar 

Shekarriz M, DeWire DM, Thomas AJ Jr, Agarwal A. A method of human semen centrifugation to minimize the iatrogenic sperm injuries caused by reactive oxygen species. Eur Urol. 1995;28(1):31–5. https://doi.org/10.1159/000475016.

Article  CAS  PubMed  Google Scholar 

Aitken RJ, De Iuliis NJ, Finnie JM, Hedges A, McLachlan RI. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria. Hum Reprod. 2010;25:2415–26. https://doi.org/10.1093/humrep/deq214.

Article  CAS  PubMed  Google Scholar 

Aitken RJ, Finnie JM, Muscio L, Whiting S, Connaughton HS, Kuczera L, et al. Potential importance of transition metals in the induction of DNA damage by sperm preparation media. Hum Reprod. 2014;29(10):2136–47. https://doi.org/10.1093/humrep/deu204.

Article  CAS  PubMed  Google Scholar 

Raad G, Bakos HW, Bazzi M, Mourad Y, Fakih F, Shayya S, et al. Differential impact of four sperm preparation techniques on sperm motility, morphology, DNA fragmentation, acrosome status, oxidative stress, and mitochondrial activity: a prospective study. Andrology. 2021;9(5):1549–59. https://doi.org/10.1111/andr.13038.

Article  CAS  PubMed 

Comments (0)

No login
gif