Gelatin Zymography of Major Proteases in Exosomes of Leishmania major Promastigotes

1. Desjeux P. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis. 2004; 27(5): 305–318.
2. Silva-Almeida M, Acacio Santini Pereira B, Lopes Ribeiro-Guimaraes M, et al. Proteinases as virulence factors in Leishmania spp. infection in mammals. Parasit and Vectors. 2012; 5(1): 160.
3. Marshall S, Kelly PH, Singh BK, Marshall R, et al. Extracellular release of virulence factor major surface protease via exosomes in Leishmania infantum promastigotes. Parasit and Vect. 2018; 11:35510.1186/s13071-018-2937.
4. Silverman JM, Chan SK, Robinson DP, et al. Proteomic analysis of the secretome of Leishmania donovani. Genome Biol .2008; 9(2): R35.
5. Smith VL, Cheng Y, Bryant BR, Schorey JS. Exosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection. Scientific Reports. 2017; 7: 43578.
6. Nuno Santarema N, Racineb G, Silvestrea R, et al. Exoproteome dynamics in Leishmania infantum. J Proteomics. 2013; 84: 106-118.
7. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259): 680-685.
8. Nabian S, Taheri M, Mazaheri Nezhad Fard R, Aramoon M. Identification of Tropomyosin and Its Immunological Properties from Larvae of Cattle Tick, Boophilus annulatus. Iran J Parasitol. 2013;8(2): 242-248.
9. Nabian S, Taheri M, Ranjbar MM , Sazmand A, Youssefy P, Nazaralipour GR. .Assessment and partial purification of serine protease inhibitors from Rhipicephalus (Boophilus) annulatus larvae. Rev Bras Parasitol Vet. 2014; 23(2): 187-93.
10. Forrest DM, Michel F, Fabricio B, et al. Proteomic analysis of exosomes derived from procyclic and metacyclic-like Cultured Leishmania infantum chagasi. J Proteomics. 2020; 227: 103902.
11. Hassani K, Shio M T, Martel C, Faubert D, Olivier M. Absence of Metalloprotease GP63 Alters the Protein Content of Leishmania Exosome. PLoS One. 2014; 9(4): e95007.
12. Cuervo P, Santos A L, Alves C R, et al. Cellular localization and expression of gp63 homologous metalloproteases in Leishmania (Viannia) braziliensis strains. Acta Trop. 2008; 106(3): 143-148.
13. Brittingham A, Morrison CJ, McMaster WR, et al. Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis. J Immunol .1995; 155(6): 3102-11.
14. Kulkarni MM, McMaster WR, Kamysz E, et al. The major surface-metalloprotease of the parasitic protozoan, Leishmania, protects against antimicrobial peptide-induced apoptotic killing. Mol Microbiol. 2006; 62(5): 1484-97.
15. Halle M, Gomez MA, Stuible M, et al. The Leishmania Surface Protease GP63 Cleaves Multiple Intracellular Proteins and Actively Participates in p38 Mitogen-activated Protein Kinase Inactivation. J Biol Chem. 2009; 284(11): 6893–6908.
16. Isnard A, Shio MT, Olivier M. Impact of Leishmania metalloprotease GP63 on macrophage signaling. Front Cell Infect Microbiol. 2012 ; 2:72.
17. Sanderson RD, Bandari SK, Vlodavsky I. Proteases and glycosidases on the surface of exosomes: Newly discovered mechanisms for extracellular remodeling. Matrix Biol. 2019; 75-76: 160–169.
18. Choudhury R, Das P, Bhaumik SK, De T, Tapati Chakraborti. In situ immunolocalization and stage-dependent expression of a secretory serine protease in Leishmania donovani and its role as a vaccine candidate. Clin Vaccine Immunol. 2010; 17(4): 660–667.
19. Swnerton RK, Knudsen GM, Sajid M, et al. Leishmania subtilisin is a maturase for the trypanothione reductase system and contributes to disease pathology. J Biol Chem. 2010; 285(41): 31120-31129.
20. Denise H, Poot J, Maribel Jimenez M, et al. Studies on the CPA cysteine peptidase in the Leishmania infantum genome strain JPCM5. BMC Mol Biol. 2006; 7: 42.
21. Silva RED, Bruna Sampaio BM, Renata Tonhosolo RT, et al. Exploring Leishmania infantum cathepsin as a new molecular marker for phylogenetic relationships and visceral leishmaniasis diagnosis. BMC Infect Dis. 2019; 19(1): 895.
22. Somanna A, Mundodi V, Gedamu LA. Functional analysis of cathepsin B-like cysteine proteases from Leishmania donovani complex. Evidence for the activation of latent transforming growth factor beta. J Biol Chem. 2002; 277(28): 25305-12.

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