Zheng YC, Guo YJ, Wang B, Wang C, Mamun MAA, Gao Y, et al. Targeting neddylation E2s: a novel therapeutic strategy in cancer. J Hematol Oncol. 2021;14:57.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wu K, Yamoah K, Dolios G, Gan-Erdene T, Tan P, Chen A, et al. DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1. J Biol Chem. 2003;278:28882–91.

Article  CAS  PubMed  Google Scholar 

Wada H, Kito K, Caskey LS, Yeh ET, Kamitani T. Cleavage of the C-terminus of NEDD8 by UCH-L3. Biochem Biophys Res Commun. 1998;251:688–92.

Article  CAS  PubMed  Google Scholar 

Huang DT, Hunt HW, Zhuang M, Ohi MD, Holton JM, Schulman BA. Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. Nature. 2007;445:394–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gong L, Yeh ET. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 1999;274:12036–42.

Article  CAS  PubMed  Google Scholar 

Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA. Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8’s E1. Mol Cell. 2005;17:341–50.

Article  CAS  PubMed  Google Scholar 

Watson IR, Irwin MS, Ohh M. NEDD8 pathways in cancer, sine quibus non. Cancer Cell. 2011;19:168–76.

Article  CAS  PubMed  Google Scholar 

Chan Y, Yoon J, Wu JT, Kim HJ, Pan KT, Yim J, et al. DEN1 deneddylates non-cullin proteins in vivo. J Cell Sci. 2008;121:3218–23.

Article  CAS  PubMed  Google Scholar 

Gong L, Kamitani T, Millas S, Yeh ET. Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins. J Biol Chem. 2000;275:14212–6.

Article  CAS  PubMed  Google Scholar 

Lyapina S, Cope G, Shevchenko A, Serino G, Tsuge T, Zhou C, et al. Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome. Science. 2001;292:1382–5.

Article  CAS  PubMed  Google Scholar 

Mendoza HM, Shen LN, Botting C, Lewis A, Chen J, Ink B, et al. NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins. J Biol Chem. 2003;278:25637–43.

Article  CAS  PubMed  Google Scholar 

Ghosh DK, Ranjan A. HYPK coordinates degradation of polyneddylated proteins by autophagy. Autophagy. 2022;18:1763–84.

Article  CAS  PubMed  Google Scholar 

Zhou L, Jiang Y, Luo Q, Li L, Jia L. Neddylation: a novel modulator of the tumor microenvironment. Mol Cancer. 2019;18:77.

Article  PubMed  PubMed Central  Google Scholar 

Wang B, Wang T, Zhu H, Yan R, Li X, Zhang C, et al. Neddylation is essential for β-catenin degradation in Wnt signaling pathway. Cell Rep. 2022;22:110538.

Article  Google Scholar 

Liakopoulos D, Doenges G, Matuschewski K, Jentsch S. A novel protein modification pathway related to the ubiquitin system. Embo J. 1998;17:2208–14.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, et al. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009;33:483–95.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gao Q, Yu GY, Shi JY, Li LH, Zhang WJ, Wang ZC, et al. Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target. Oncotarget. 2014;5:7820–32.

Article  PubMed  PubMed Central  Google Scholar 

Li L, Wang M, Yu G, Chen P, Li H, Wei D, et al. Overactivated neddylation pathway as a therapeutic target in lung cancer. J Natl Cancer Inst. 2014;106:dju083.

Article  PubMed  Google Scholar 

Cukras S, Morffy N, Ohn T, Kee Y. Inactivating UBE2M impacts the DNA damage response and genome integrity involving multiple cullin ligases. PLoS One. 2014;9:e101844.

Article  PubMed  PubMed Central  Google Scholar 

Zhang Y, Shi CC, Zhang HP, Li GQ, Li SS. MLN4924 suppresses neddylation and induces cell cycle arrest, senescence, and apoptosis in human osteosarcoma. Oncotarget. 2016;7:45263–74.

Article  PubMed  PubMed Central  Google Scholar 

Zhang G-C, Yu X-N, Sun J-L, Xiong J, Yang Y-J, Jiang X-M, et al. UBE2M promotes cell proliferation via the β-catenin/cyclin D1 signaling in hepatocellular carcinoma. Aging. 2020;12:2373–92.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jayabalan AK, Sanchez A, Park RY, Yoon SP, Kang GY, Baek JH, et al. NEDDylation promotes stress granule assembly. Nat Commun. 2016;7:12125.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lu G, Yi J, Gubas A, Wang YT, Wu Y, Ren Y, et al. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. Autophagy. 2019;15:1917–34.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhao B, Gao C, Shi D, Mao J, Zhao J, Guo L, et al. Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells. Oncol Rep. 2019;42:2670–9.

CAS  PubMed  Google Scholar 

Brown JS, Lukashchuk N, Sczaniecka-Clift M, Britton S, le Sage C, Calsou P, et al. Neddylation promotes ubiquitylation and release of Ku from DNA-damage sites. Cell Rep. 2015;11:704–14.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhao X, Jiang L, Hu D, Tang Y, Zhao G, Du X, et al. NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation. Exp Cell Res. 2021;403:112614.

Article  CAS  PubMed  Google Scholar 

Xu J, Lv G, Xu B, Jiang B. Overexpression of UBE2M through Wnt/β-Catenin signaling is associated with poor prognosis and chemotherapy resistance in colorectal cancer. Transl Cancer Res. 2020;9:5614–25.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhou W, Xu J, Li H, Xu M, Chen ZJ, Wei W, et al. Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkage. Clin Cancer Res. 2017;23:1104–16.

Article  CAS  PubMed  Google Scholar 

Hopf LVM, Baek K, Klügel M, von Gronau S, Xiong Y, Schulman BA. Structure of CRL7(FBXW8) reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation. Nat Struct Mol Biol. 2022;29:854–62.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Soucy TA, Smith PG, Milhollen MA, Berger AJ, Gavin JM, Adhikari S, et al. An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer. Nature. 2009;458:732–6.

Article  CAS  PubMed  Google Scholar 

Milhollen MA, Thomas MP, Narayanan U, Traore T, Riceberg J, Amidon BS, et al. Treatment-emergent mutations in NAEβ confer resistance to the NEDD8-activating enzyme inhibitor MLN4924. Cancer Cell. 2012;21:388–401.

Article  CAS  PubMed  Google Scholar 

Pugh CW, Ratcliffe PJ. The von Hippel-Lindau tumor suppressor, hypoxia-inducible factor-1 (HIF-1) degradation, and cancer pathogenesis. Semin Cancer Biol. 2003;13:83–9.

Article  CAS  PubMed  Google Scholar 

Taguchi K, Yamamoto M. The KEAP1-NRF2 system as a molecular target of cancer treatment. Cancers. 2020;13:46.

Article  PubMed  PubMed Central  Google Scholar 

Burroughs AM, Jaffee M, Iyer LM, Aravind L. Anatomy of the E2 ligase fold: implications for enzymology and evolution of ubiquitin/Ub-like protein conjugation. J Struct Biol. 2008;162:205–18.

Article  CAS  PubMed  PubMed Central  Google Scholar 

van Wijk SJ, Timmers HT. The family of ubiquitin-conjugating enzymes (E2s): deciding between life and death of proteins. FASEB J. 2010;24:981–93.

Article  PubMed  Google Scholar 

Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF, et al. A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. Nat Struct Mol Biol. 2004;11:927–35.

Article  CAS  PubMed