Although dual X-ray absorptiometry (DXA) has been used to determine total body volume, using DXA to determine regional (i.e., arm and leg) volumes needs further assessment. Thus, the aim of the present study is to evaluate the validity of total and regional DXA-derived body volume compared to a traditional method for measuring body volume.

A total of 30 males and females (Age: 25.9 ± 4.0 yrs.; Height: 1.75 ± 0.10 m; Weight: 70.98 ± 14.02 kg) underwent one whole body DXA scan, underwater weighing, and regional measures of volume via water displacement. Manually created DXA region of interest boxes were used to determine regional DXA body composition. Total body volume was calculated by taking the participant’s dry weight and dividing it by the average density from underwater weighing. Linear regression models with body volume from underwater weighing for total body volume and water displacement for regional volume as the dependent variable and DXA lean mass, fat mass, and bone mass as independent variables created total and regional DXA-derived body volume. T-tests assessed DXA-derived body volume to the traditional method of body volume assessment. Regression models were cross-validated using the Repeated k-fold Cross Validation method.

DXA-derived total body volume was not significantly (p=0.999) different from total body volume measured via total body water displacement. In addition, both arm and leg regional DXA-derived volume was not significantly different (p=0.999) compared to regional volume measured by regional water displacement. Cross-validation of each model produced R2 values of 0.992, 0.923, and 0.932 for total body, arm, and leg, respectively.

The DXA may be used as valid method for estimating total and regional body volume. Thus, these results expand the DXA’s capabilities and potentially allow for a convenient regional four-compartment model with DXA-derived regional volume.